Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897597758> ?p ?o ?g. }
- W2897597758 endingPage "15123" @default.
- W2897597758 startingPage "15114" @default.
- W2897597758 abstract "Chemical biology research often requires precise covalent attachment of labels to the native proteins. Such methods are sought after to probe, design, and regulate the properties of proteins. At present, this demand is largely unmet due to the lack of empowering chemical technology. Here, we report a chemical platform that enables site-selective labeling of native proteins. Initially, a reversible intermolecular reaction places the “chemical linchpins” globally on all the accessible Lys residues. These linchpins have the capability to drive site-selective covalent labeling of proteins. The linchpin detaches within physiological conditions and capacitates the late-stage installation of various tags. The chemical platform is modular, and the reagent design regulates the site of modification. The linchpin is a multitasking group and facilitates purification of the labeled protein eliminating the requirement of additional chromatography tag. The methodology allows the labeling of a single protein in a mixture of proteins. The precise modification of an accessible residue in protein ensures that their structure remains unaltered. The enzymatic activity of myoglobin, cytochrome C, aldolase, and lysozyme C remains conserved after labeling. Also, the cellular uptake of modified insulin and its downstream signaling process remain unperturbed. The linchpin directed modification (LDM) provides a convenient route for the conjugation of a fluorophore and drug to a Fab and monoclonal antibody. It delivers trastuzumab-doxorubicin and trastuzumab-emtansine conjugates with selective antiproliferative activity toward Her-2 positive SKBR-3 breast cancer cells." @default.
- W2897597758 created "2018-10-26" @default.
- W2897597758 creator A5003054128 @default.
- W2897597758 creator A5027004184 @default.
- W2897597758 creator A5033095334 @default.
- W2897597758 creator A5039971841 @default.
- W2897597758 creator A5046663677 @default.
- W2897597758 creator A5054209786 @default.
- W2897597758 creator A5057763339 @default.
- W2897597758 creator A5059547647 @default.
- W2897597758 creator A5062072610 @default.
- W2897597758 date "2018-10-17" @default.
- W2897597758 modified "2023-09-24" @default.
- W2897597758 title "Single-Site Labeling of Native Proteins Enabled by a Chemoselective and Site-Selective Chemical Technology" @default.
- W2897597758 cites W1556223086 @default.
- W2897597758 cites W1589169334 @default.
- W2897597758 cites W1976892990 @default.
- W2897597758 cites W1978513278 @default.
- W2897597758 cites W1982205639 @default.
- W2897597758 cites W1983639577 @default.
- W2897597758 cites W1984111577 @default.
- W2897597758 cites W1987957230 @default.
- W2897597758 cites W1987966136 @default.
- W2897597758 cites W1989914749 @default.
- W2897597758 cites W2003460765 @default.
- W2897597758 cites W2008601485 @default.
- W2897597758 cites W2015442731 @default.
- W2897597758 cites W2017835340 @default.
- W2897597758 cites W2021320094 @default.
- W2897597758 cites W2023034663 @default.
- W2897597758 cites W2025470424 @default.
- W2897597758 cites W2029932803 @default.
- W2897597758 cites W2041710436 @default.
- W2897597758 cites W2041806467 @default.
- W2897597758 cites W2043594245 @default.
- W2897597758 cites W2044677774 @default.
- W2897597758 cites W2046385606 @default.
- W2897597758 cites W2055204581 @default.
- W2897597758 cites W2060746246 @default.
- W2897597758 cites W2061020069 @default.
- W2897597758 cites W2068258715 @default.
- W2897597758 cites W2069685762 @default.
- W2897597758 cites W2075303826 @default.
- W2897597758 cites W2090682502 @default.
- W2897597758 cites W2096081574 @default.
- W2897597758 cites W2107167859 @default.
- W2897597758 cites W2108403040 @default.
- W2897597758 cites W2114043686 @default.
- W2897597758 cites W2115427274 @default.
- W2897597758 cites W2116150122 @default.
- W2897597758 cites W2124514746 @default.
- W2897597758 cites W2128876853 @default.
- W2897597758 cites W2135572502 @default.
- W2897597758 cites W2137250659 @default.
- W2897597758 cites W2139296689 @default.
- W2897597758 cites W2147533449 @default.
- W2897597758 cites W2151709132 @default.
- W2897597758 cites W2165168809 @default.
- W2897597758 cites W2183643866 @default.
- W2897597758 cites W2254963963 @default.
- W2897597758 cites W2258806502 @default.
- W2897597758 cites W2272264121 @default.
- W2897597758 cites W2292564527 @default.
- W2897597758 cites W2316589566 @default.
- W2897597758 cites W2316959718 @default.
- W2897597758 cites W2336972286 @default.
- W2897597758 cites W2395903385 @default.
- W2897597758 cites W2418946054 @default.
- W2897597758 cites W2562259593 @default.
- W2897597758 cites W2568910234 @default.
- W2897597758 cites W2586934570 @default.
- W2897597758 cites W2597045666 @default.
- W2897597758 cites W2789882464 @default.
- W2897597758 cites W2790553494 @default.
- W2897597758 cites W2805816883 @default.
- W2897597758 cites W4213262935 @default.
- W2897597758 doi "https://doi.org/10.1021/jacs.8b10490" @default.
- W2897597758 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30336012" @default.
- W2897597758 hasPublicationYear "2018" @default.
- W2897597758 type Work @default.
- W2897597758 sameAs 2897597758 @default.
- W2897597758 citedByCount "95" @default.
- W2897597758 countsByYear W28975977582018 @default.
- W2897597758 countsByYear W28975977582019 @default.
- W2897597758 countsByYear W28975977582020 @default.
- W2897597758 countsByYear W28975977582021 @default.
- W2897597758 countsByYear W28975977582022 @default.
- W2897597758 countsByYear W28975977582023 @default.
- W2897597758 crossrefType "journal-article" @default.
- W2897597758 hasAuthorship W2897597758A5003054128 @default.
- W2897597758 hasAuthorship W2897597758A5027004184 @default.
- W2897597758 hasAuthorship W2897597758A5033095334 @default.
- W2897597758 hasAuthorship W2897597758A5039971841 @default.
- W2897597758 hasAuthorship W2897597758A5046663677 @default.
- W2897597758 hasAuthorship W2897597758A5054209786 @default.
- W2897597758 hasAuthorship W2897597758A5057763339 @default.
- W2897597758 hasAuthorship W2897597758A5059547647 @default.
- W2897597758 hasAuthorship W2897597758A5062072610 @default.