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- W2897612893 abstract "Robust cellular bioenergetics is vital in the energy-demanding process of maintaining matrix homeostasis in the intervertebral disc. Age-related decline in disc cellular bioenergetics is hypothesised to contribute to the matrix homeostatic perturbation observed in intervertebral disc degeneration. The present study aimed to measure how ageing impacted disc cell mitochondria and bioenergetics. Age-related changes measured included matrix content and cellularity in disc tissue, as well as matrix synthesis, cell proliferation and senescence markers in cell cultures derived from annulus fibrosus (AF) and nucleus pulposus (NP) isolated from the discs of young (6-9 months) and older (36-50 months) New Zealand White rabbits. Cellular bioenergetic parameters were measured using a Seahorse XFe96 Analyzer, in addition to quantitating mitochondrial morphological changes and membrane potential. Ageing reduced mitochondrial number and membrane potential in both cell types. Also, it significantly reduced glycolytic capacity, mitochondrial reserve capacity, maximum aerobic capacity and non-glucose-dependent respiration in NP. Moreover, NP cells exhibited age-related decline in matrix synthesis and reduced cellularity in older tissues. Despite a lack of changes in mitochondrial respiration with age, AF cells showed an increase in glycolysis and altered matrix production. While previous studies report age-related matrix degenerative changes in disc cells, the present study revealed, for the first time, that ageing affected mitochondrial number and function, particularly in NP cells. Consequently, age-related bioenergetic changes may contribute to the functional alterations in aged NP cells that underlie disc degeneration." @default.
- W2897612893 created "2018-10-26" @default.
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- W2897612893 date "2018-10-18" @default.
- W2897612893 modified "2023-10-02" @default.
- W2897612893 title "Age-dependent changes in intervertebral disc cell mitochondria and bioenergetics" @default.
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- W2897612893 cites W1790117378 @default.
- W2897612893 cites W1826389851 @default.
- W2897612893 cites W1894046416 @default.
- W2897612893 cites W1964126982 @default.
- W2897612893 cites W1965310616 @default.
- W2897612893 cites W1978822037 @default.
- W2897612893 cites W1979669395 @default.
- W2897612893 cites W1980064174 @default.
- W2897612893 cites W1994654873 @default.
- W2897612893 cites W1994867329 @default.
- W2897612893 cites W1994877694 @default.
- W2897612893 cites W2000723582 @default.
- W2897612893 cites W2012533194 @default.
- W2897612893 cites W2018641577 @default.
- W2897612893 cites W2020293812 @default.
- W2897612893 cites W2029337331 @default.
- W2897612893 cites W2029500622 @default.
- W2897612893 cites W2030604088 @default.
- W2897612893 cites W2031536699 @default.
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- W2897612893 cites W2034614591 @default.
- W2897612893 cites W2037600829 @default.
- W2897612893 cites W2042967670 @default.
- W2897612893 cites W2055966310 @default.
- W2897612893 cites W2060861339 @default.
- W2897612893 cites W2065433522 @default.
- W2897612893 cites W2066108134 @default.
- W2897612893 cites W2067229758 @default.
- W2897612893 cites W2071262681 @default.
- W2897612893 cites W2075015150 @default.
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- W2897612893 cites W2090421014 @default.
- W2897612893 cites W2095297866 @default.
- W2897612893 cites W2104908670 @default.
- W2897612893 cites W2109011367 @default.
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- W2897612893 cites W2148495041 @default.
- W2897612893 cites W2151559508 @default.
- W2897612893 cites W2152983876 @default.
- W2897612893 cites W2207074962 @default.
- W2897612893 cites W2267737556 @default.
- W2897612893 cites W2281598305 @default.
- W2897612893 cites W2303721616 @default.
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- W2897612893 cites W2461813906 @default.
- W2897612893 cites W2466392202 @default.
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- W2897612893 cites W2508367806 @default.
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- W2897612893 doi "https://doi.org/10.22203/ecm.v036a13" @default.
- W2897612893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30334244" @default.
- W2897612893 hasPublicationYear "2018" @default.
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