Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897615043> ?p ?o ?g. }
- W2897615043 endingPage "e0205527" @default.
- W2897615043 startingPage "e0205527" @default.
- W2897615043 abstract "Nowadays, most reverse genetics approaches in Trypanosoma brucei, a protozoan parasite of medical and veterinary importance, rely on pre-established cell lines. Consequently, inducible experimentation is reduced to a few laboratory strains. Here we described a new transgene expression system based exclusively on endogenous transcription activities and a minimum set of regulatory components that can easily been adapted to different strains. The pTbFIX vectors are designed to contain the sequence of interest under the control of an inducible rRNA promoter along with a constitutive dicistronic unit encoding a nucleus targeted tetracycline repressor and puromycin resistance genes in a tandem “head-to-tail” configuration. Upon doxycycline induction, the system supports regulatable GFP expression (170 to 400 fold) in both bloodstream and procyclic T. brucei forms. Furthermore we have adapted the pTbFIX plasmid to perform RNAi experimentation. Lethal phenotypes, including α-tubulin and those corresponding to the enolase and clathrin heavy chain genes, were successfully recapitulated in procyclic and bloodstream parasites thus showing the versatility of this new tool." @default.
- W2897615043 created "2018-10-26" @default.
- W2897615043 creator A5008148904 @default.
- W2897615043 creator A5049091535 @default.
- W2897615043 creator A5062306850 @default.
- W2897615043 creator A5080532244 @default.
- W2897615043 date "2018-10-11" @default.
- W2897615043 modified "2023-10-18" @default.
- W2897615043 title "Simplified inducible system for Trypanosoma brucei" @default.
- W2897615043 cites W104844502 @default.
- W2897615043 cites W1482068783 @default.
- W2897615043 cites W1583868806 @default.
- W2897615043 cites W1840360079 @default.
- W2897615043 cites W1963617455 @default.
- W2897615043 cites W1966772537 @default.
- W2897615043 cites W1979391949 @default.
- W2897615043 cites W1994844474 @default.
- W2897615043 cites W1995467090 @default.
- W2897615043 cites W2002331297 @default.
- W2897615043 cites W2008099529 @default.
- W2897615043 cites W2014565441 @default.
- W2897615043 cites W2017577540 @default.
- W2897615043 cites W2021033833 @default.
- W2897615043 cites W2025355301 @default.
- W2897615043 cites W2027990348 @default.
- W2897615043 cites W2028292566 @default.
- W2897615043 cites W2036780183 @default.
- W2897615043 cites W2044101358 @default.
- W2897615043 cites W2045067781 @default.
- W2897615043 cites W2059778243 @default.
- W2897615043 cites W2067728537 @default.
- W2897615043 cites W2067777264 @default.
- W2897615043 cites W2074620204 @default.
- W2897615043 cites W2078073818 @default.
- W2897615043 cites W2078216835 @default.
- W2897615043 cites W2087860032 @default.
- W2897615043 cites W2088352540 @default.
- W2897615043 cites W2088834030 @default.
- W2897615043 cites W2093562204 @default.
- W2897615043 cites W2093822146 @default.
- W2897615043 cites W2102565252 @default.
- W2897615043 cites W2105699548 @default.
- W2897615043 cites W2108256794 @default.
- W2897615043 cites W2116350092 @default.
- W2897615043 cites W2121293790 @default.
- W2897615043 cites W2130712328 @default.
- W2897615043 cites W2136711098 @default.
- W2897615043 cites W2153023888 @default.
- W2897615043 cites W2157236463 @default.
- W2897615043 cites W2160570153 @default.
- W2897615043 cites W2161761672 @default.
- W2897615043 cites W2317185681 @default.
- W2897615043 cites W2395646452 @default.
- W2897615043 cites W2492867627 @default.
- W2897615043 cites W2606323899 @default.
- W2897615043 cites W2781131614 @default.
- W2897615043 cites W2809221219 @default.
- W2897615043 cites W4240696919 @default.
- W2897615043 doi "https://doi.org/10.1371/journal.pone.0205527" @default.
- W2897615043 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6181392" @default.
- W2897615043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30308039" @default.
- W2897615043 hasPublicationYear "2018" @default.
- W2897615043 type Work @default.
- W2897615043 sameAs 2897615043 @default.
- W2897615043 citedByCount "6" @default.
- W2897615043 countsByYear W28976150432020 @default.
- W2897615043 countsByYear W28976150432021 @default.
- W2897615043 countsByYear W28976150432022 @default.
- W2897615043 countsByYear W28976150432023 @default.
- W2897615043 crossrefType "journal-article" @default.
- W2897615043 hasAuthorship W2897615043A5008148904 @default.
- W2897615043 hasAuthorship W2897615043A5049091535 @default.
- W2897615043 hasAuthorship W2897615043A5062306850 @default.
- W2897615043 hasAuthorship W2897615043A5080532244 @default.
- W2897615043 hasBestOaLocation W28976150431 @default.
- W2897615043 hasConcept C102230213 @default.
- W2897615043 hasConcept C104317684 @default.
- W2897615043 hasConcept C111829913 @default.
- W2897615043 hasConcept C166703698 @default.
- W2897615043 hasConcept C2779502636 @default.
- W2897615043 hasConcept C54355233 @default.
- W2897615043 hasConcept C67705224 @default.
- W2897615043 hasConcept C86803240 @default.
- W2897615043 hasConcept C95444343 @default.
- W2897615043 hasConceptScore W2897615043C102230213 @default.
- W2897615043 hasConceptScore W2897615043C104317684 @default.
- W2897615043 hasConceptScore W2897615043C111829913 @default.
- W2897615043 hasConceptScore W2897615043C166703698 @default.
- W2897615043 hasConceptScore W2897615043C2779502636 @default.
- W2897615043 hasConceptScore W2897615043C54355233 @default.
- W2897615043 hasConceptScore W2897615043C67705224 @default.
- W2897615043 hasConceptScore W2897615043C86803240 @default.
- W2897615043 hasConceptScore W2897615043C95444343 @default.
- W2897615043 hasFunder F4320321594 @default.
- W2897615043 hasFunder F4320325364 @default.
- W2897615043 hasFunder F4320334832 @default.
- W2897615043 hasIssue "10" @default.
- W2897615043 hasLocation W28976150431 @default.