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• W2897628066 abstract "Maternal consumption of a high-fat diet (HFD) during pregnancy has established adverse effects on the developing neonate. In this study, we aimed to investigate the effect of an HFD on the murine mammary gland during midlactation. Female C57BL/6J mice were placed on either a low-fat diet (LFD/10% fat) or HFD (60% fat) from 3 wk of age through peak lactation (lactation day 11/L11). After 4 wk of consuming either the LFD or HFD, female mice were bred. There were no significant differences in milk yield between treatment groups, which was measured from L1 to L9. On L10, mice were subjected to an overnight fast and then euthanized on the morning of L11. Total RNA was isolated from inguinal mammary glands for whole transcriptome sequencing. We found 628 genes that were differentially expressed between the treatment groups. Notably, HFD feeding resulted in expression alterations of genes involved in collagen and cytoplasmic components. Additionally, genes related to inflammatory and immune responses were also impacted. Differential expression in gene transcript isoforms between the treatment groups was detected in three genes related to mammary duct development. This study sheds light as to how an HFD may affect the mammary gland transcriptome during midlactation." @default.
• W2897628066 created "2018-10-26" @default.
• W2897628066 creator A5055512363 @default.
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• W2897628066 date "2018-12-01" @default.
• W2897628066 modified "2023-09-27" @default.
• W2897628066 title "Effect of high-fat diet feeding and associated transcriptome changes in the peak lactation mammary gland in C57BL/6 dams" @default.
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• W2897628066 doi "https://doi.org/10.1152/physiolgenomics.00052.2018" @default.
• W2897628066 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30339477" @default.
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