FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897648462> ?p ?o ?g. }

• W2897648462 endingPage "87" @default.
• W2897648462 startingPage "82" @default.
• W2897648462 abstract "We report a lab-on-a-chip immunosesnor for quantification of the inflammatory cytokine TNF-α with picomolar sensitivity. The feasibility of the technology was demonstrated via accurate measurement of the concentration of TNF-α in astrocytes cell culture media. The immunoassay was performed in a microfluidic device with an integrated antimony/bismuth thermopile sensor and had a limit of detection of 14 pg mL−1. The device was fabricated using rapid prototyping xurography technique and consisted of two inlets and single outlet. Anti-TNF-α monoclonal antibody was used to capture the analyte while the detection was performed using glucose oxidase-conjugated secondary antibody. Glucose (55 mM) was injected through a sample loop into the fluid flowing within the microfluidic device. A nanovolt meter connected to the thermoelectric sensor recorded the voltage change caused by the enzymatic reaction. Computer simulations using COMSOL Multiphysics were performed to analyze the effect of fluid velocity on the concentration of glucose within the reaction zone. A standard calibration curve was created using serial dilutions of synthetic TNF-α (0–2000 pg mL−1) by plotting the area under the curve of the signal versus the concentration of the analyte. The efficacy of the device was evaluated by quantifying TNF-α in the cell culture medium of lipopolysaccharide stimulated and non-stimulated astrocytes. The results demonstrated high accuracy of the calorimetric immunoassay when compared with gold standard commercial ELISA microplate reader. The immunosensor offers excellent reproducibility, accuracy, and versatility in the choice of the detection enzyme." @default.
• W2897648462 created "2018-10-26" @default.
• W2897648462 creator A5071589954 @default.
• W2897648462 creator A5073621298 @default.
• W2897648462 creator A5084723525 @default.
• W2897648462 date "2019-02-01" @default.
• W2897648462 modified "2023-10-12" @default.
• W2897648462 title "Calorimetric sandwich-type immunosensor for quantification of TNF-α" @default.
• W2897648462 cites W1536204744 @default.
• W2897648462 cites W1971629816 @default.
• W2897648462 cites W1975525336 @default.
• W2897648462 cites W1978212782 @default.
• W2897648462 cites W1982667720 @default.
• W2897648462 cites W1983586473 @default.
• W2897648462 cites W1984466510 @default.
• W2897648462 cites W1984716652 @default.
• W2897648462 cites W2004846144 @default.
• W2897648462 cites W2015759485 @default.
• W2897648462 cites W2020965771 @default.
• W2897648462 cites W2035726424 @default.
• W2897648462 cites W2037988112 @default.
• W2897648462 cites W2047092425 @default.
• W2897648462 cites W2051243774 @default.
• W2897648462 cites W2063744999 @default.
• W2897648462 cites W2070072418 @default.
• W2897648462 cites W2071757735 @default.
• W2897648462 cites W2075741372 @default.
• W2897648462 cites W2077416294 @default.
• W2897648462 cites W2079775054 @default.
• W2897648462 cites W2084951848 @default.
• W2897648462 cites W2106330541 @default.
• W2897648462 cites W2109311842 @default.
• W2897648462 cites W2123095919 @default.
• W2897648462 cites W2147589521 @default.
• W2897648462 cites W2155213995 @default.
• W2897648462 cites W2161016084 @default.
• W2897648462 cites W2168037016 @default.
• W2897648462 cites W2515730548 @default.
• W2897648462 cites W2620640983 @default.
• W2897648462 cites W2735666441 @default.
• W2897648462 doi "https://doi.org/10.1016/j.bios.2018.10.028" @default.
• W2897648462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30396021" @default.
• W2897648462 hasPublicationYear "2019" @default.
• W2897648462 type Work @default.
• W2897648462 sameAs 2897648462 @default.
• W2897648462 citedByCount "29" @default.
• W2897648462 countsByYear W28976484622019 @default.
• W2897648462 countsByYear W28976484622020 @default.
• W2897648462 countsByYear W28976484622021 @default.
• W2897648462 countsByYear W28976484622022 @default.
• W2897648462 countsByYear W28976484622023 @default.
• W2897648462 crossrefType "journal-article" @default.
• W2897648462 hasAuthorship W2897648462A5071589954 @default.
• W2897648462 hasAuthorship W2897648462A5073621298 @default.
• W2897648462 hasAuthorship W2897648462A5084723525 @default.
• W2897648462 hasConcept C10390740 @default.
• W2897648462 hasConcept C105795698 @default.
• W2897648462 hasConcept C113196181 @default.
• W2897648462 hasConcept C119128265 @default.
• W2897648462 hasConcept C120665830 @default.
• W2897648462 hasConcept C121332964 @default.
• W2897648462 hasConcept C138942068 @default.
• W2897648462 hasConcept C142724271 @default.
• W2897648462 hasConcept C158355884 @default.
• W2897648462 hasConcept C159654299 @default.
• W2897648462 hasConcept C160756335 @default.
• W2897648462 hasConcept C165838908 @default.
• W2897648462 hasConcept C171250308 @default.
• W2897648462 hasConcept C184112429 @default.
• W2897648462 hasConcept C185592680 @default.
• W2897648462 hasConcept C192562407 @default.
• W2897648462 hasConcept C203014093 @default.
• W2897648462 hasConcept C204787440 @default.
• W2897648462 hasConcept C23531484 @default.
• W2897648462 hasConcept C2780420688 @default.
• W2897648462 hasConcept C33923547 @default.
• W2897648462 hasConcept C43617362 @default.
• W2897648462 hasConcept C47279676 @default.
• W2897648462 hasConcept C70784841 @default.
• W2897648462 hasConcept C71924100 @default.
• W2897648462 hasConcept C8673954 @default.
• W2897648462 hasConcept C86803240 @default.
• W2897648462 hasConcept C9893847 @default.
• W2897648462 hasConceptScore W2897648462C10390740 @default.
• W2897648462 hasConceptScore W2897648462C105795698 @default.
• W2897648462 hasConceptScore W2897648462C113196181 @default.
• W2897648462 hasConceptScore W2897648462C119128265 @default.
• W2897648462 hasConceptScore W2897648462C120665830 @default.
• W2897648462 hasConceptScore W2897648462C121332964 @default.
• W2897648462 hasConceptScore W2897648462C138942068 @default.
• W2897648462 hasConceptScore W2897648462C142724271 @default.
• W2897648462 hasConceptScore W2897648462C158355884 @default.
• W2897648462 hasConceptScore W2897648462C159654299 @default.
• W2897648462 hasConceptScore W2897648462C160756335 @default.
• W2897648462 hasConceptScore W2897648462C165838908 @default.
• W2897648462 hasConceptScore W2897648462C171250308 @default.
• W2897648462 hasConceptScore W2897648462C184112429 @default.
• W2897648462 hasConceptScore W2897648462C185592680 @default.

• next