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• W2897710208 abstract "Abstract Despite the importance of Y‐chromosomes in evolution and sex determination, their heterochromatic, repeat‐rich nature makes them difficult to sequence (due, in part, to ambiguities in sequence alignment and assembly) and to genetically manipulate. Therefore, they generally remain poorly understood. For example, the Drosophila melanogaster Y‐chromosome, one of the most extensively studied Y‐chromosomes, is widely heterochromatic and composed mainly of highly repetitive sequences, with only a handful of expressed genes scattered throughout its length. Efforts to insert transgenes on this chromosome have thus far relied on either random insertion of transposons (sometimes harbouring ‘landing sites’ for subsequent integrations) with limited success or on chromosomal translocations, thereby limiting the types of Y‐chromosome‐related questions that could be explored. Here, we describe a versatile approach to site‐specifically insert transgenes on the Y‐chromosome in D. melanogaster via CRISPR/Cas9‐mediated homology‐directed repair. We demonstrate the ability to insert, and detect expression from, fluorescently marked transgenes at two specific locations on the Y‐chromosome, and we utilize these marked Y‐chromosomes to detect and quantify rare chromosomal nondisjunction effects. Finally, we discuss how this Y‐docking technique could be adapted to other insects to aid in the development of genetic control technologies for the management of insect disease vectors and pests." @default.
• W2897710208 created "2018-10-26" @default.
• W2897710208 creator A5037711069 @default.
• W2897710208 creator A5082263103 @default.
• W2897710208 date "2018-10-08" @default.
• W2897710208 modified "2023-10-05" @default.
• W2897710208 title "Site‐specific transgenesis of the <i>Drosophila melanogaster</i> Y‐chromosome using CRISPR/Cas9" @default.
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• W2897710208 doi "https://doi.org/10.1111/imb.12528" @default.
• W2897710208 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8459378" @default.
• W2897710208 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30079589" @default.
• W2897710208 hasPublicationYear "2018" @default.
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