Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897726819> ?p ?o ?g. }
- W2897726819 endingPage "60" @default.
- W2897726819 startingPage "39" @default.
- W2897726819 abstract "Prominin‐1 is a cell surface biomarker that allows the identification of stem and cancer stem cells from different organs. It is also expressed in several differentiated epithelial and non‐epithelial cells. Irrespective of the cell type, prominin‐1 is associated with plasma membrane protrusions. Here, we investigate its impact on the architecture of membrane protrusions using microvilli of Madin‐Darby canine kidney cells as the main model. Our high‐resolution analysis revealed that upon the overexpression of prominin‐1 the number of microvilli and clusters of them increased. Microvilli with branched and/or knob‐like morphologies were observed and stimulated by mutations in the ganglioside‐binding site of prominin‐1. The altered phenotypes were caused by the interaction of prominin‐1 with phosphoinositide 3‐kinase and Arp2/3 complex. Mutation of tyrosine 828 of prominin‐1 impaired its phosphorylation and thereby inhibited the aforementioned interactions abolishing altered microvilli. This suggests that the interplay of prominin‐1‐ganglioside membrane complexes, phosphoinositide 3‐kinase and cytoskeleton components regulates microvillar architecture. Lastly, the expression of prominin‐1 and its mutants modified the structure of filopodia emerging from fibroblast‐like cells and silencing human prominin‐1 in primary hematopoietic stem cells resulted in the loss of uropod‐associated microvilli. Altogether, these findings strengthen the role of prominin‐1 as an organizer of cellular protrusions." @default.
- W2897726819 created "2018-10-26" @default.
- W2897726819 creator A5026849709 @default.
- W2897726819 creator A5027036729 @default.
- W2897726819 creator A5029249438 @default.
- W2897726819 creator A5045007233 @default.
- W2897726819 creator A5047837173 @default.
- W2897726819 creator A5072500215 @default.
- W2897726819 creator A5073142143 @default.
- W2897726819 creator A5079667888 @default.
- W2897726819 creator A5082128151 @default.
- W2897726819 creator A5086638684 @default.
- W2897726819 date "2018-11-14" @default.
- W2897726819 modified "2023-10-15" @default.
- W2897726819 title "Prominin‐1 (CD133) modulates the architecture and dynamics of microvilli" @default.
- W2897726819 cites W133253872 @default.
- W2897726819 cites W1838077380 @default.
- W2897726819 cites W1960862471 @default.
- W2897726819 cites W1964367767 @default.
- W2897726819 cites W1968116483 @default.
- W2897726819 cites W1970508239 @default.
- W2897726819 cites W1972110025 @default.
- W2897726819 cites W1973933701 @default.
- W2897726819 cites W1974081806 @default.
- W2897726819 cites W1974270860 @default.
- W2897726819 cites W1976402706 @default.
- W2897726819 cites W1978677736 @default.
- W2897726819 cites W1981682592 @default.
- W2897726819 cites W1982380864 @default.
- W2897726819 cites W1987119582 @default.
- W2897726819 cites W1989249682 @default.
- W2897726819 cites W1998888750 @default.
- W2897726819 cites W2000400490 @default.
- W2897726819 cites W2001519992 @default.
- W2897726819 cites W2001544973 @default.
- W2897726819 cites W2003143907 @default.
- W2897726819 cites W2003591869 @default.
- W2897726819 cites W2003616045 @default.
- W2897726819 cites W2003968931 @default.
- W2897726819 cites W2010781810 @default.
- W2897726819 cites W2011470992 @default.
- W2897726819 cites W2013285760 @default.
- W2897726819 cites W2013902799 @default.
- W2897726819 cites W2013933111 @default.
- W2897726819 cites W2016544021 @default.
- W2897726819 cites W2023293152 @default.
- W2897726819 cites W2023965702 @default.
- W2897726819 cites W2025371958 @default.
- W2897726819 cites W2029275169 @default.
- W2897726819 cites W2030066338 @default.
- W2897726819 cites W2033363947 @default.
- W2897726819 cites W2035994012 @default.
- W2897726819 cites W2038004616 @default.
- W2897726819 cites W2040566182 @default.
- W2897726819 cites W2045274781 @default.
- W2897726819 cites W2046093759 @default.
- W2897726819 cites W2048663988 @default.
- W2897726819 cites W2048887344 @default.
- W2897726819 cites W2048976218 @default.
- W2897726819 cites W2067785731 @default.
- W2897726819 cites W2074388799 @default.
- W2897726819 cites W2075871987 @default.
- W2897726819 cites W2077760991 @default.
- W2897726819 cites W2079458697 @default.
- W2897726819 cites W2080231804 @default.
- W2897726819 cites W2080744444 @default.
- W2897726819 cites W2081479357 @default.
- W2897726819 cites W2083069189 @default.
- W2897726819 cites W2084343793 @default.
- W2897726819 cites W2084344183 @default.
- W2897726819 cites W2095401091 @default.
- W2897726819 cites W2098649843 @default.
- W2897726819 cites W2099802095 @default.
- W2897726819 cites W2099908647 @default.
- W2897726819 cites W2103629395 @default.
- W2897726819 cites W2108140552 @default.
- W2897726819 cites W2108372612 @default.
- W2897726819 cites W2108653140 @default.
- W2897726819 cites W2110145708 @default.
- W2897726819 cites W2110315172 @default.
- W2897726819 cites W2112866667 @default.
- W2897726819 cites W2113544773 @default.
- W2897726819 cites W2117735955 @default.
- W2897726819 cites W2122782850 @default.
- W2897726819 cites W2127792983 @default.
- W2897726819 cites W2128345511 @default.
- W2897726819 cites W2133691499 @default.
- W2897726819 cites W2139319389 @default.
- W2897726819 cites W2146134298 @default.
- W2897726819 cites W2149246707 @default.
- W2897726819 cites W2149795523 @default.
- W2897726819 cites W2151271433 @default.
- W2897726819 cites W2156232698 @default.
- W2897726819 cites W2157047159 @default.
- W2897726819 cites W2158804607 @default.
- W2897726819 cites W2159637527 @default.
- W2897726819 cites W2162017151 @default.
- W2897726819 cites W2162413201 @default.