FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897743478> ?p ?o ?g. }

• W2897743478 abstract "Summary The present study examines the role of RNA polymerase I (RPI)-mediated transcription, maternally inherited rRNA and nucleolar proteins in the resumption of fibrillogranular nucleoli during embryonic genome activation (EGA) in porcine embryos. Late 4-cell embryos were incubated in the absence (control) or presence of actinomycin D (AD) (0.2 μg/ml for inhibition of RPI; 2.0 μg/ml for inhibition of total transcription) and late 2-cell embryos were cultured to the late 4-cell stage with 0.2 μg/ml AD to block EGA. Embryos were then processed for reverse-transcriptase polymerase chain reaction (RT-PCR), and for autoradiography (ARG), transmission electron microscopy (TEM), fluorescence in situ hybridization (FISH), silver staining and immunofluorescence (for RPI). Embryos in the control group displayed extranucleolar and intranucleolar ARG labelling, and exhibited de novo synthesis of rRNA and reticulated functional nucleoli. Nucleolar proteins were located in large foci. After RPI inhibition, nucleolar precursors transformed into segregated fibrillogranular structures, however no fibrillar centres were observed. The localization of rDNA and clusters of rRNA were detected in 57.1% immunoprecipitated (IP) analyzed nucleoli and dispersed RPI; 30.5% of nuclei showed large deposits of nucleolar proteins. Embryos from the AD-2.0 group did not display any transcriptional activity. Nucleolar formation was completely blocked, however 39.4% of nuclei showed rRNA clusters; 85.7% of nuclei were co-localized with nucleolar proteins. Long-term transcriptional inhibition resulted in the lack of ARG and RPI labelling; 40% of analyzed nuclei displayed the accumulation of rRNA molecules into large foci. In conclusion, maternally inherited rRNA co-localized with rDNA and nucleolar proteins can initiate a partial nucleolar assembly, resulting in the formation of fibrilogranular structures independently on activation of RPI-mediated transcription." @default.
• W2897743478 created "2018-10-26" @default.
• W2897743478 creator A5000121261 @default.
• W2897743478 creator A5003253363 @default.
• W2897743478 creator A5006071734 @default.
• W2897743478 creator A5009920620 @default.
• W2897743478 creator A5019864770 @default.
• W2897743478 creator A5031506593 @default.
• W2897743478 creator A5032438511 @default.
• W2897743478 creator A5064975863 @default.
• W2897743478 creator A5073821191 @default.
• W2897743478 creator A5086091501 @default.
• W2897743478 date "2018-10-01" @default.
• W2897743478 modified "2023-09-23" @default.
• W2897743478 title "Maternally inherited rRNA triggers de novo nucleolus formation in porcine embryos" @default.
• W2897743478 cites W1789492119 @default.
• W2897743478 cites W1869558150 @default.
• W2897743478 cites W1964888903 @default.
• W2897743478 cites W1968320822 @default.
• W2897743478 cites W1985088076 @default.
• W2897743478 cites W1987933963 @default.
• W2897743478 cites W1989462515 @default.
• W2897743478 cites W1991468531 @default.
• W2897743478 cites W2000111119 @default.
• W2897743478 cites W2000362412 @default.
• W2897743478 cites W2003388570 @default.
• W2897743478 cites W2007497552 @default.
• W2897743478 cites W2015949500 @default.
• W2897743478 cites W2018462308 @default.
• W2897743478 cites W2029300932 @default.
• W2897743478 cites W2034254435 @default.
• W2897743478 cites W2036373513 @default.
• W2897743478 cites W2049431525 @default.
• W2897743478 cites W2060760567 @default.
• W2897743478 cites W2065456224 @default.
• W2897743478 cites W2069359878 @default.
• W2897743478 cites W2070922557 @default.
• W2897743478 cites W2078628283 @default.
• W2897743478 cites W2086601894 @default.
• W2897743478 cites W2086701922 @default.
• W2897743478 cites W2093315651 @default.
• W2897743478 cites W2099309498 @default.
• W2897743478 cites W2103863868 @default.
• W2897743478 cites W2113919360 @default.
• W2897743478 cites W2119477130 @default.
• W2897743478 cites W2120965724 @default.
• W2897743478 cites W2127450718 @default.
• W2897743478 cites W2143371973 @default.
• W2897743478 cites W2156094225 @default.
• W2897743478 cites W2159338695 @default.
• W2897743478 cites W2165710621 @default.
• W2897743478 cites W2168343536 @default.
• W2897743478 cites W2169783225 @default.
• W2897743478 cites W2296542523 @default.
• W2897743478 cites W2305205176 @default.
• W2897743478 cites W2314196166 @default.
• W2897743478 cites W2325258348 @default.
• W2897743478 doi "https://doi.org/10.1017/s0967199418000400" @default.
• W2897743478 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30311594" @default.
• W2897743478 hasPublicationYear "2018" @default.
• W2897743478 type Work @default.
• W2897743478 sameAs 2897743478 @default.
• W2897743478 citedByCount "0" @default.
• W2897743478 crossrefType "journal-article" @default.
• W2897743478 hasAuthorship W2897743478A5000121261 @default.
• W2897743478 hasAuthorship W2897743478A5003253363 @default.
• W2897743478 hasAuthorship W2897743478A5006071734 @default.
• W2897743478 hasAuthorship W2897743478A5009920620 @default.
• W2897743478 hasAuthorship W2897743478A5019864770 @default.
• W2897743478 hasAuthorship W2897743478A5031506593 @default.
• W2897743478 hasAuthorship W2897743478A5032438511 @default.
• W2897743478 hasAuthorship W2897743478A5064975863 @default.
• W2897743478 hasAuthorship W2897743478A5073821191 @default.
• W2897743478 hasAuthorship W2897743478A5086091501 @default.
• W2897743478 hasConcept C104317684 @default.
• W2897743478 hasConcept C138885662 @default.
• W2897743478 hasConcept C153911025 @default.
• W2897743478 hasConcept C179926584 @default.
• W2897743478 hasConcept C183873130 @default.
• W2897743478 hasConcept C185592680 @default.
• W2897743478 hasConcept C196843134 @default.
• W2897743478 hasConcept C25281209 @default.
• W2897743478 hasConcept C2776449523 @default.
• W2897743478 hasConcept C2780723820 @default.
• W2897743478 hasConcept C41895202 @default.
• W2897743478 hasConcept C55493867 @default.
• W2897743478 hasConcept C67705224 @default.
• W2897743478 hasConcept C67905577 @default.
• W2897743478 hasConcept C86803240 @default.
• W2897743478 hasConcept C95444343 @default.
• W2897743478 hasConceptScore W2897743478C104317684 @default.
• W2897743478 hasConceptScore W2897743478C138885662 @default.
• W2897743478 hasConceptScore W2897743478C153911025 @default.
• W2897743478 hasConceptScore W2897743478C179926584 @default.
• W2897743478 hasConceptScore W2897743478C183873130 @default.
• W2897743478 hasConceptScore W2897743478C185592680 @default.
• W2897743478 hasConceptScore W2897743478C196843134 @default.
• W2897743478 hasConceptScore W2897743478C25281209 @default.
• W2897743478 hasConceptScore W2897743478C2776449523 @default.
• W2897743478 hasConceptScore W2897743478C2780723820 @default.

• next