Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897747202> ?p ?o ?g. }
- W2897747202 endingPage "442" @default.
- W2897747202 startingPage "433" @default.
- W2897747202 abstract "As a complex and common post-translational modification, N-linked glycosylation affects a recombinant glycoprotein's biological activity and efficacy. For example, the α1,6-fucosylation significantly affects antibody-dependent cellular cytotoxicity and α2,6-sialylation is critical for antibody anti-inflammatory activity. Terminal sialylation is important for a glycoprotein's circulatory half-life. Chinese hamster ovary (CHO) cells are currently the predominant recombinant protein production platform, and, in this review, the characteristics of CHO glycosylation are summarized. Moreover, recent and current metabolic engineering strategies for tailoring glycoprotein fucosylation and sialylation in CHO cells, intensely investigated in the past decades, are described. One approach for reducing α1,6-fucosylation is through inhibiting fucosyltransferase (FUT8) expression by knockdown and knockout methods. Another approach to modulate fucosylation is through inhibition of multiple genes in the fucosylation biosynthesis pathway or through chemical inhibitors. To modulate antibody sialylation of the fragment crystallizable region, expressions of sialyltransferase and galactotransferase individually or together with amino acid mutations can affect antibody glycoforms and further influence antibody effector functions. The inhibition of sialidase expression and chemical supplementations are also effective and complementary approaches to improve the sialylation levels on recombinant glycoproteins. The engineering of CHO cells or protein sequence to control glycoforms to produce more homogenous glycans is an emerging topic. For modulating the glycosylation metabolic pathways, the interplay of multiple glyco-gene knockouts and knockins and the combination of multiple approaches, including genetic manipulation, protein engineering and chemical supplementation, are detailed in order to achieve specific glycan profiles on recombinant glycoproteins for superior biological function and effectiveness." @default.
- W2897747202 created "2018-10-26" @default.
- W2897747202 creator A5000044234 @default.
- W2897747202 creator A5014510444 @default.
- W2897747202 date "2018-10-18" @default.
- W2897747202 modified "2023-09-27" @default.
- W2897747202 title "Metabolic engineering of CHO cells to prepare glycoproteins" @default.
- W2897747202 cites W111187742 @default.
- W2897747202 cites W122291305 @default.
- W2897747202 cites W1514815542 @default.
- W2897747202 cites W1518819083 @default.
- W2897747202 cites W1534648775 @default.
- W2897747202 cites W1539242187 @default.
- W2897747202 cites W1565489899 @default.
- W2897747202 cites W1582703272 @default.
- W2897747202 cites W1592578892 @default.
- W2897747202 cites W1676407394 @default.
- W2897747202 cites W1764586703 @default.
- W2897747202 cites W1830102282 @default.
- W2897747202 cites W1874142858 @default.
- W2897747202 cites W1886156337 @default.
- W2897747202 cites W1897249225 @default.
- W2897747202 cites W1908224577 @default.
- W2897747202 cites W1929486047 @default.
- W2897747202 cites W1966892299 @default.
- W2897747202 cites W1973113001 @default.
- W2897747202 cites W1975380390 @default.
- W2897747202 cites W1982131071 @default.
- W2897747202 cites W1986197388 @default.
- W2897747202 cites W1989407795 @default.
- W2897747202 cites W2005158488 @default.
- W2897747202 cites W2006994535 @default.
- W2897747202 cites W2013529902 @default.
- W2897747202 cites W2014649183 @default.
- W2897747202 cites W2017239895 @default.
- W2897747202 cites W2023831371 @default.
- W2897747202 cites W2027747719 @default.
- W2897747202 cites W2031963644 @default.
- W2897747202 cites W2035433348 @default.
- W2897747202 cites W2039083746 @default.
- W2897747202 cites W2047755908 @default.
- W2897747202 cites W2050376954 @default.
- W2897747202 cites W2052231275 @default.
- W2897747202 cites W2055237989 @default.
- W2897747202 cites W2055465436 @default.
- W2897747202 cites W2059347528 @default.
- W2897747202 cites W2069942860 @default.
- W2897747202 cites W2079579628 @default.
- W2897747202 cites W2082247235 @default.
- W2897747202 cites W2083441961 @default.
- W2897747202 cites W2084136419 @default.
- W2897747202 cites W2085734421 @default.
- W2897747202 cites W2086820922 @default.
- W2897747202 cites W2092329879 @default.
- W2897747202 cites W2093343938 @default.
- W2897747202 cites W2094771394 @default.
- W2897747202 cites W2096168733 @default.
- W2897747202 cites W2100518445 @default.
- W2897747202 cites W2108223798 @default.
- W2897747202 cites W2110568229 @default.
- W2897747202 cites W2115571948 @default.
- W2897747202 cites W2123900742 @default.
- W2897747202 cites W2124847934 @default.
- W2897747202 cites W2126371691 @default.
- W2897747202 cites W2128015484 @default.
- W2897747202 cites W2129658913 @default.
- W2897747202 cites W2136722681 @default.
- W2897747202 cites W2137354517 @default.
- W2897747202 cites W2140035322 @default.
- W2897747202 cites W2141073877 @default.
- W2897747202 cites W2142660163 @default.
- W2897747202 cites W2146981871 @default.
- W2897747202 cites W2148306650 @default.
- W2897747202 cites W2152125983 @default.
- W2897747202 cites W2152155460 @default.
- W2897747202 cites W2156750419 @default.
- W2897747202 cites W2160798258 @default.
- W2897747202 cites W2168072557 @default.
- W2897747202 cites W2175128997 @default.
- W2897747202 cites W2414473807 @default.
- W2897747202 cites W2436001939 @default.
- W2897747202 cites W2528678937 @default.
- W2897747202 cites W2563879789 @default.
- W2897747202 cites W2588114451 @default.
- W2897747202 cites W2603910151 @default.
- W2897747202 cites W2724308281 @default.
- W2897747202 cites W2769461495 @default.
- W2897747202 cites W2779082428 @default.
- W2897747202 cites W2792245399 @default.
- W2897747202 cites W2793600336 @default.
- W2897747202 cites W4240129365 @default.
- W2897747202 cites W4256391593 @default.
- W2897747202 cites W4361865139 @default.
- W2897747202 cites W993338644 @default.
- W2897747202 doi "https://doi.org/10.1042/etls20180056" @default.
- W2897747202 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33525787" @default.
- W2897747202 hasPublicationYear "2018" @default.
- W2897747202 type Work @default.