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- W2897748811 abstract "Summary A high‐throughput method (≥ 10 6 of clones can be analysed on a single agar plate) for the selection of ester‐hydrolysing enzymes was developed based on the uridine auxotrophy of Escherichia coli strain DH 10B Δpyr FEC and the acylated derivatives 2′,3′,5′‐ O ‐tri‐acetyluridine and 2′,3′,5′‐ O ‐tri‐hexanoyluridine as the sole source of uridine. The proposed approach permits the selection of hydrolases belonging to different families and active towards different substrates. Moreover, the ester group of the substrate used for the selection, at least partly, determined the specificity of the selected enzymes." @default.
- W2897748811 created "2018-10-26" @default.
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- W2897748811 date "2018-10-09" @default.
- W2897748811 modified "2023-10-18" @default.
- W2897748811 title "Application of the uridine auxotrophic host and synthetic nucleosides for a rapid selection of hydrolases from metagenomic libraries" @default.
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- W2897748811 doi "https://doi.org/10.1111/1751-7915.13316" @default.
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