FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897749385> ?p ?o ?g. }

• W2897749385 abstract "Photoconvertible fluorescent proteins (PCFPs) are widely used as markers for the visualization of intracellular processes and for sub-diffraction single-molecule localization microscopy. Although wild type of a new photoconvertible fluorescent protein SAASoti tends to aggregate, we succeeded, via rational mutagenesis, to obtain variants that formed either tetramers or monomers. We compare two approaches: one is based on the structural similarity between SAASoti and Kaede, which helped us to identify a single point mutation (V127T) at the protein's hydrophobic interface that leads to monomerization. The other is based on a chemical modification of amino groups of SAASoti with succinic anhydride, which converts the protein aggregates into monomers. Mass-spectrometric analysis helped us to identify that the modification of a single ε-amino group of lysine K145 in the strongly charged interface AB was sufficient to convert the protein into its tetrameric form. Furthermore, site-directed mutagenesis was used to generate mutants that proved to be either monomeric or tetrameric, both capable of rapid green-to-red photoconversion. This allows SAASoti to be used as a photoconvertible fluorescent marker for in vivo cell studies." @default.
• W2897749385 created "2018-10-26" @default.
• W2897749385 creator A5021149467 @default.
• W2897749385 creator A5022006378 @default.
• W2897749385 creator A5028258766 @default.
• W2897749385 creator A5034349557 @default.
• W2897749385 creator A5038695717 @default.
• W2897749385 creator A5042353808 @default.
• W2897749385 creator A5051162684 @default.
• W2897749385 creator A5051411855 @default.
• W2897749385 creator A5054507148 @default.
• W2897749385 creator A5070104377 @default.
• W2897749385 creator A5083940469 @default.
• W2897749385 date "2018-10-19" @default.
• W2897749385 modified "2023-10-14" @default.
• W2897749385 title "Monomerization of the photoconvertible fluorescent protein SAASoti by rational mutagenesis of single amino acids" @default.
• W2897749385 cites W1504622816 @default.
• W2897749385 cites W1538873343 @default.
• W2897749385 cites W1544900705 @default.
• W2897749385 cites W1782900133 @default.
• W2897749385 cites W1972289308 @default.
• W2897749385 cites W1974413011 @default.
• W2897749385 cites W1982442386 @default.
• W2897749385 cites W1983616620 @default.
• W2897749385 cites W1989239695 @default.
• W2897749385 cites W1995702879 @default.
• W2897749385 cites W1996426210 @default.
• W2897749385 cites W2017962200 @default.
• W2897749385 cites W2022246087 @default.
• W2897749385 cites W2028309801 @default.
• W2897749385 cites W2029309165 @default.
• W2897749385 cites W2029667189 @default.
• W2897749385 cites W2054455698 @default.
• W2897749385 cites W2058527555 @default.
• W2897749385 cites W2059322936 @default.
• W2897749385 cites W2071172673 @default.
• W2897749385 cites W2071647127 @default.
• W2897749385 cites W2073939942 @default.
• W2897749385 cites W2076112920 @default.
• W2897749385 cites W2080419619 @default.
• W2897749385 cites W2101115746 @default.
• W2897749385 cites W2101336703 @default.
• W2897749385 cites W2102174161 @default.
• W2897749385 cites W2105332737 @default.
• W2897749385 cites W2107913200 @default.
• W2897749385 cites W2108407770 @default.
• W2897749385 cites W2112274012 @default.
• W2897749385 cites W2116435209 @default.
• W2897749385 cites W2118611156 @default.
• W2897749385 cites W2120504118 @default.
• W2897749385 cites W2146621245 @default.
• W2897749385 cites W2148493274 @default.
• W2897749385 cites W2151334331 @default.
• W2897749385 cites W2153924661 @default.
• W2897749385 cites W2161422945 @default.
• W2897749385 cites W2162725456 @default.
• W2897749385 cites W2164738046 @default.
• W2897749385 cites W2167258646 @default.
• W2897749385 cites W2521039464 @default.
• W2897749385 cites W2730565525 @default.
• W2897749385 cites W2789926421 @default.
• W2897749385 doi "https://doi.org/10.1038/s41598-018-33250-z" @default.
• W2897749385 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6195611" @default.
• W2897749385 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30341334" @default.
• W2897749385 hasPublicationYear "2018" @default.
• W2897749385 type Work @default.
• W2897749385 sameAs 2897749385 @default.
• W2897749385 citedByCount "8" @default.
• W2897749385 countsByYear W28977493852019 @default.
• W2897749385 countsByYear W28977493852021 @default.
• W2897749385 countsByYear W28977493852022 @default.
• W2897749385 countsByYear W28977493852023 @default.
• W2897749385 crossrefType "journal-article" @default.
• W2897749385 hasAuthorship W2897749385A5021149467 @default.
• W2897749385 hasAuthorship W2897749385A5022006378 @default.
• W2897749385 hasAuthorship W2897749385A5028258766 @default.
• W2897749385 hasAuthorship W2897749385A5034349557 @default.
• W2897749385 hasAuthorship W2897749385A5038695717 @default.
• W2897749385 hasAuthorship W2897749385A5042353808 @default.
• W2897749385 hasAuthorship W2897749385A5051162684 @default.
• W2897749385 hasAuthorship W2897749385A5051411855 @default.
• W2897749385 hasAuthorship W2897749385A5054507148 @default.
• W2897749385 hasAuthorship W2897749385A5070104377 @default.
• W2897749385 hasAuthorship W2897749385A5083940469 @default.
• W2897749385 hasBestOaLocation W28977493851 @default.
• W2897749385 hasConcept C104317684 @default.
• W2897749385 hasConcept C121332964 @default.
• W2897749385 hasConcept C12554922 @default.
• W2897749385 hasConcept C143065580 @default.
• W2897749385 hasConcept C16318435 @default.
• W2897749385 hasConcept C166940927 @default.
• W2897749385 hasConcept C178790620 @default.
• W2897749385 hasConcept C185592680 @default.
• W2897749385 hasConcept C2777735259 @default.
• W2897749385 hasConcept C2778067846 @default.
• W2897749385 hasConcept C515207424 @default.
• W2897749385 hasConcept C521977710 @default.
• W2897749385 hasConcept C53105671 @default.
• W2897749385 hasConcept C54355233 @default.
• W2897749385 hasConcept C55493867 @default.

• next