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- W2897782255 abstract "Taxol is a blockbuster anticancer drug that commercially synthesized from 10-decaetylbaccatin III intermediate of Taxus baccata, however, this technology has been challenged by reproducibility and association of unwanted compounds. Recently, endophytic fungi with fast growing and feasibility of molecular engineering raise the hope for sustainable production of Taxol, unfortunately the loss of their productivity with multiple subculturing is the main challenge to authenticate this approach industrially. Thus, searching for novel fungal isolates with molecular and metabolic functional stability for Taxol production was the objective of this work. Among the experimented fungal isolates, Aspergillus flavipes, a soil-dwelling saprophyte, gave the highest Taxol yield (185 μg/l). Metabolically, A. flavipes had a strong stability for Taxol production till the 10th subcultures (185-160 μg/l) on the same cultural conditions. The yield of Taxol by A. flavipes was increased by 5 folds (850 μg/ L) with fluconazole (1.0 μg/ml) addition to 5 days old culture. The expression of Taxol biosynthetic genes ts, dbat, bapt and transcriptional factor pbcR was significantly increased by 8–10 folds upon fluconazole addition that correlates with Taxol yield. HSP70 was induced by 13 folds with addition of fluconazole to cultures of A. flavipes. Thus, Taxol biosynthesis could be transcriptionally regulated by pbcR and post-translationally modified by HSP70 chaperones. To the best of knowledge, this is the first report exploring the potency of saprophytic A. flavipes for Taxol production with metabolic stability with multiple subculturing." @default.
- W2897782255 created "2018-10-26" @default.
- W2897782255 creator A5021105458 @default.
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- W2897782255 creator A5050040054 @default.
- W2897782255 creator A5065519829 @default.
- W2897782255 creator A5068395471 @default.
- W2897782255 date "2019-01-01" @default.
- W2897782255 modified "2023-09-27" @default.
- W2897782255 title "Sterol inhibitor “Fluconazole” enhance the Taxol yield and molecular expression of its encoding genes cluster from Aspergillus flavipes" @default.
- W2897782255 cites W1519266993 @default.
- W2897782255 cites W1560827310 @default.
- W2897782255 cites W1735246829 @default.
- W2897782255 cites W1860256737 @default.
- W2897782255 cites W1888485107 @default.
- W2897782255 cites W1971384143 @default.
- W2897782255 cites W1972736746 @default.
- W2897782255 cites W1975533327 @default.
- W2897782255 cites W1977310841 @default.
- W2897782255 cites W1980012367 @default.
- W2897782255 cites W1985381418 @default.
- W2897782255 cites W1986607177 @default.
- W2897782255 cites W1990565169 @default.
- W2897782255 cites W1991366673 @default.
- W2897782255 cites W1995314551 @default.
- W2897782255 cites W1995380559 @default.
- W2897782255 cites W1996806381 @default.
- W2897782255 cites W1998966075 @default.
- W2897782255 cites W1999161681 @default.
- W2897782255 cites W1999881814 @default.
- W2897782255 cites W2000472204 @default.
- W2897782255 cites W2001605890 @default.
- W2897782255 cites W2010490244 @default.
- W2897782255 cites W2012170494 @default.
- W2897782255 cites W2014138939 @default.
- W2897782255 cites W2018237934 @default.
- W2897782255 cites W2021295704 @default.
- W2897782255 cites W2029352277 @default.
- W2897782255 cites W2030793893 @default.
- W2897782255 cites W2037490361 @default.
- W2897782255 cites W2038271952 @default.
- W2897782255 cites W2045009247 @default.
- W2897782255 cites W2045507047 @default.
- W2897782255 cites W2045798970 @default.
- W2897782255 cites W2050289749 @default.
- W2897782255 cites W2055823349 @default.
- W2897782255 cites W2056934915 @default.
- W2897782255 cites W2058518669 @default.
- W2897782255 cites W2060198978 @default.
- W2897782255 cites W2063313990 @default.
- W2897782255 cites W2063396371 @default.
- W2897782255 cites W2064773124 @default.
- W2897782255 cites W2067233533 @default.
- W2897782255 cites W2067299834 @default.
- W2897782255 cites W2069678406 @default.
- W2897782255 cites W2071431306 @default.
- W2897782255 cites W2072888427 @default.
- W2897782255 cites W2075843047 @default.
- W2897782255 cites W2075934694 @default.
- W2897782255 cites W2077637394 @default.
- W2897782255 cites W2078417397 @default.
- W2897782255 cites W2097035467 @default.
- W2897782255 cites W2097785134 @default.
- W2897782255 cites W2099368167 @default.
- W2897782255 cites W2100837269 @default.
- W2897782255 cites W2102955607 @default.
- W2897782255 cites W2106346045 @default.
- W2897782255 cites W2108313755 @default.
- W2897782255 cites W2121884322 @default.
- W2897782255 cites W2125121305 @default.
- W2897782255 cites W2140609225 @default.
- W2897782255 cites W2141563185 @default.
- W2897782255 cites W2145535413 @default.
- W2897782255 cites W2149477112 @default.
- W2897782255 cites W2152063809 @default.
- W2897782255 cites W2153177881 @default.
- W2897782255 cites W2153206234 @default.
- W2897782255 cites W2153415111 @default.
- W2897782255 cites W2154958527 @default.
- W2897782255 cites W2161699606 @default.
- W2897782255 cites W2165636230 @default.
- W2897782255 cites W2167741650 @default.
- W2897782255 cites W2169846040 @default.
- W2897782255 cites W2269208301 @default.
- W2897782255 cites W2322393094 @default.
- W2897782255 cites W2340594887 @default.
- W2897782255 cites W2540831465 @default.
- W2897782255 cites W2604178846 @default.
- W2897782255 cites W2800945929 @default.
- W2897782255 cites W2949341415 @default.
- W2897782255 doi "https://doi.org/10.1016/j.procbio.2018.10.008" @default.
- W2897782255 hasPublicationYear "2019" @default.
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