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• W2897787848 abstract "The role of astrocytes is becoming increasingly important to understanding how glioblastoma (GBM) tumor cells diffusely invade the brain. Yet, little is known of the contribution of extracellular vesicle (EV) signaling in GBM/astrocyte interactions. We modeled GBM-EV signaling to normal astrocytes in vitro to assess whether this mode of intercellular communication could support GBM progression. EVs were isolated and characterized from three patient-derived GBM stem cells (NES+/CD133+) and their differentiated (diff) progeny cells (NES−/CD133−). Uptake of GBM-EVs by normal primary astrocytes was confirmed by fluorescence microscopy, and changes in astrocyte podosome formation and gelatin degradation were measured. Quantitative mass spectrometry-based proteomics was performed on GBM-EV stimulated astrocytes. Interaction networks were generated from common, differentially abundant proteins using Ingenuity® (Qiagen Bioinformatics) and predicted upstream regulators were tested by qPCR assays. Podosome formation and Cy3-gelatin degradation were induced in astrocytes following 24-h exposure to GBM-stem and -diff EVs, with EVs released by GBM-stem cells eliciting a greater effect. More than 1700 proteins were quantified, and bioinformatics predicted activations of MYC, NFE2L2, FN1, and TGFβ1 and inhibition of TP53 in GBM-EV stimulated astrocytes that were then confirmed by qPCR. Further qPCR studies identified significantly decreased Δ133p53 and increased p53β in astrocytes exposed to GBM-EVs that might indicate the acquisition of a pro-inflammatory, tumor-promoting senescence-associated secretory phenotype (SASP). Inhibition of TP53 and activation of MYC signaling pathways in normal astrocytes exposed to GBM-EVs may be a mechanism by which GBM manipulates astrocytes to acquire a phenotype that promotes tumor progression." @default.
• W2897787848 created "2018-10-26" @default.
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• W2897787848 date "2018-10-23" @default.
• W2897787848 modified "2023-10-18" @default.
• W2897787848 title "Extracellular Vesicles Released by Glioblastoma Cells Stimulate Normal Astrocytes to Acquire a Tumor-Supportive Phenotype Via p53 and MYC Signaling Pathways" @default.
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• W2897787848 cites W1536836107 @default.
• W2897787848 cites W1547644303 @default.
• W2897787848 cites W1552807847 @default.
• W2897787848 cites W1797058518 @default.
• W2897787848 cites W1963051938 @default.
• W2897787848 cites W1964227273 @default.
• W2897787848 cites W1964232997 @default.
• W2897787848 cites W1979975126 @default.
• W2897787848 cites W1980393580 @default.
• W2897787848 cites W1981001306 @default.
• W2897787848 cites W1981339473 @default.
• W2897787848 cites W1984557698 @default.
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• W2897787848 cites W2007382415 @default.
• W2897787848 cites W2015556811 @default.
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• W2897787848 cites W2019318549 @default.
• W2897787848 cites W2021536610 @default.
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• W2897787848 cites W2025183726 @default.
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• W2897787848 cites W2035833975 @default.
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• W2897787848 cites W2054536623 @default.
• W2897787848 cites W2062180415 @default.
• W2897787848 cites W2062448230 @default.
• W2897787848 cites W2081124475 @default.
• W2897787848 cites W2081758137 @default.
• W2897787848 cites W2085898732 @default.
• W2897787848 cites W2087956154 @default.
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• W2897787848 cites W2141569879 @default.
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• W2897787848 doi "https://doi.org/10.1007/s12035-018-1385-1" @default.
• W2897787848 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6505517" @default.
• W2897787848 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30353492" @default.
• W2897787848 hasPublicationYear "2018" @default.
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