FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897790143> ?p ?o ?g. }

• W2897790143 abstract "Epithelial migration plays an important role during re-epithelization phase in wound healing. Several skin diseases, such as chronic ulcers, fail during this stage. During last years a new insight into this problem has arisen introducing a mechanical point of view of this process. Kinematics and density distribution within the epithelium play key role during collective migration. Besides, the capacity of cells to proliferate and provide the issue with new cells enhance the ability of them closing the wound. The evolution of the leading edge have shown that experiments performed at starvation stopped at some point whereas those performed at normal condition kept advancing. A deeper study of the velocity fields shown that, cells several rows behind the edge barely moved during the whole experiment. The experiments without proliferation shown a sudden increase in velocity that rapidly decayed after some hours while experiments at normal conditions keep a constant velocity during the whole time, although it also decreased. Although velocity profiles showed a completely different behaviour between experiments performed at normal conditions and at starvation, the study of density distribution of cells inside the tissue exhibit almost the same profiles. This result lead to the idea that cells acquire a well established profile to move, ’advancing formation’, with lower density at the front where cells with greater velocity are found and many rows behind, confluent cells, which display slow velocity. Cell migration results from the interplay of mechanical and chemical interactions between cells and their extracellular environment. Mathematical in silico models can reproduce experimental results and can predict different future behaviors. Due to the complexity and huge amount of data obtained from in vitro experiments, an emergent need of bioinformatics and mathematical models have appeared although experiments will always be needed. The main advantage is their ability to handle multiple interacting variables simultaneously, which is difficult to deal with in experiments. Here, we introduce the novelty of density field into Banerjee et al. model with the polarization force term proposed by Arciero et al which mimics better our experimental results and is more physiological. The combination of both has provided good results for the starvation case studied. The temporal evolution of the monolayer length has been fitted to obtain the parameters of the model to be later used to calculate the main variables governing the problem, displacement, density and concentration of contractile units. The real size of the cell culture has shown to be an important factor to be under consideration in regulating the density distribution. Besides, the influence study of several parameters of the model have shown to exert little influence on the solution. Diffusivity or internal pressure could be then removed from the model without influencing significantly the results. Transepithelial potential (TEP) is the voltage across an epithelium which is the sum of all the voltages of single cell membranes. After epidermis wounding, this transepithelial potential is disrupted inducing endogeneous epithelial electric fields (EEF) that might be implicated in wound re-epithelization. In this work we present an epithelial cell monolayer electrical characterization by means of impedance spectroscopy techniques. We have study a wide range of frequencies and three different voltages leading to the conclusion that cell monolayer exhibit a linear impedance behaviour. Given that results we propose an equivalent electric circuit to better understand cell-cell and cell-substrate communications. Besides, wound healing assays were performed and external electric fields were applied to force different migration rates improving the ability of cells to migrate towards the wound. Finally, internal calcium concentration was monitored for the different external voltages applied showing higher concentration in those experiments performed with the largest electric field at the beginning of the experiments. This result suggests that cell-cell communication is enhanced by electric fields improving ion exchange and therefore improving migration rates. This thesis has provided an important knowledge in the physics governing cell migration to Termeg group. We have started a new research line studying collective cell migration, mechanical forces or electric fields involved in the process of wound healing in vitro." @default.
• W2897790143 created "2018-10-26" @default.
• W2897790143 creator A5073725706 @default.
• W2897790143 date "2017-01-01" @default.
• W2897790143 modified "2023-09-27" @default.
• W2897790143 title "Characterizing the mechanical response of epidermal cell monolayers during wound healing" @default.
• W2897790143 hasPublicationYear "2017" @default.
• W2897790143 type Work @default.
• W2897790143 sameAs 2897790143 @default.
• W2897790143 citedByCount "0" @default.
• W2897790143 crossrefType "journal-article" @default.
• W2897790143 hasAuthorship W2897790143A5073725706 @default.
• W2897790143 hasConcept C121332964 @default.
• W2897790143 hasConcept C12554922 @default.
• W2897790143 hasConcept C137738243 @default.
• W2897790143 hasConcept C1491633281 @default.
• W2897790143 hasConcept C185592680 @default.
• W2897790143 hasConcept C203014093 @default.
• W2897790143 hasConcept C2780269544 @default.
• W2897790143 hasConcept C529295009 @default.
• W2897790143 hasConcept C54355233 @default.
• W2897790143 hasConcept C55493867 @default.
• W2897790143 hasConcept C57879066 @default.
• W2897790143 hasConcept C86803240 @default.
• W2897790143 hasConcept C95444343 @default.
• W2897790143 hasConceptScore W2897790143C121332964 @default.
• W2897790143 hasConceptScore W2897790143C12554922 @default.
• W2897790143 hasConceptScore W2897790143C137738243 @default.
• W2897790143 hasConceptScore W2897790143C1491633281 @default.
• W2897790143 hasConceptScore W2897790143C185592680 @default.
• W2897790143 hasConceptScore W2897790143C203014093 @default.
• W2897790143 hasConceptScore W2897790143C2780269544 @default.
• W2897790143 hasConceptScore W2897790143C529295009 @default.
• W2897790143 hasConceptScore W2897790143C54355233 @default.
• W2897790143 hasConceptScore W2897790143C55493867 @default.
• W2897790143 hasConceptScore W2897790143C57879066 @default.
• W2897790143 hasConceptScore W2897790143C86803240 @default.
• W2897790143 hasConceptScore W2897790143C95444343 @default.
• W2897790143 hasLocation W28977901431 @default.
• W2897790143 hasOpenAccess W2897790143 @default.
• W2897790143 hasPrimaryLocation W28977901431 @default.
• W2897790143 hasRelatedWork W142873892 @default.
• W2897790143 hasRelatedWork W1535255717 @default.
• W2897790143 hasRelatedWork W1907126089 @default.
• W2897790143 hasRelatedWork W1982376467 @default.
• W2897790143 hasRelatedWork W1995129166 @default.
• W2897790143 hasRelatedWork W2009337022 @default.
• W2897790143 hasRelatedWork W2083704676 @default.
• W2897790143 hasRelatedWork W2103180356 @default.
• W2897790143 hasRelatedWork W2162658340 @default.
• W2897790143 hasRelatedWork W2298347346 @default.
• W2897790143 hasRelatedWork W2327733045 @default.
• W2897790143 hasRelatedWork W2419460157 @default.
• W2897790143 hasRelatedWork W2802983537 @default.
• W2897790143 hasRelatedWork W2899634416 @default.
• W2897790143 hasRelatedWork W2947411978 @default.
• W2897790143 hasRelatedWork W2950824030 @default.
• W2897790143 hasRelatedWork W2981485529 @default.
• W2897790143 hasRelatedWork W2996634850 @default.
• W2897790143 hasRelatedWork W3034522888 @default.
• W2897790143 hasRelatedWork W3101693860 @default.
• W2897790143 isParatext "false" @default.
• W2897790143 isRetracted "false" @default.
• W2897790143 magId "2897790143" @default.
• W2897790143 workType "article" @default.