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• W2897812529 abstract "1506 Hyaluronan (HA) is a non-sulfated glycosamioglycan that is vastly present in the extracellular matrix contributing to tissue homeostasis and structural integrity. The pathological relevance of HA to cancer has been extensively studied in many types of malignancies. Higher levels of HA in the tumor-stroma interface has been known to promote the tumor progression and metastasis through the constitutive HA-CD44 interactions and their related intracellular signalling pathways. We therefore hypothesize that reduction of extracellular HA by the catabolic process using hyaluronidase would attenuate the interaction of HA with CD44 receptor, and therefore decrease the rate of cancer cell proliferation and invasion. In this study, Streptococcus pyogenes bacteriophage H4489A hyaluronidase (HylP) and its mutant HylP H262A have been expressed and purified to determine their effects on human breast cancer. Three different breast cancer cell lines (Hs578T, MDA-MB-231 and MCF-7) were chosen based on their HA production levels, and the colorimetric XTT assay and Matrigel invasion assay were performed to evaluate the effect of HylP on cell proliferation and invasion respectively. To further investigate the effect of the reduction in HA level on signalling pathways, the expression and activation of Erk1/2, Akt, RhoA, and metalloprotease were measured by immunoblotting and zymographic methods.
 Upon treatment with HylP, decrement of both cell proliferation and invasion of aggressive breast cancer cell lines (Hs578T and MDA-MB-231) were observed, whereas the non-invasive cancer cells (MCF-7) showed no effect. The decrease in cell proliferation was proportional to the level of HA produced by the cell lines. The reduction of HA by HylP was shown to down-regulate the signaling pathways for cancer cell proliferation and invasion (Erk1/2, Akt, and RhoA). In the case of the mutant HylP H262A that exhibits limited enzymatic activity, no such response was observed. These results suggest that the observed inhibitory effect of HylP is exerted by the reduction of HA. Furthermore, the external addition of longer HA polymers increased the invasiveness of both Hs578T and MDA-MB-231, however, when treated with HylP, a drastic reduction in cell invasion was observed. In accordance with cell invasion assays, the activation of MMP-2 was facilitated upon the addition of HA and its activation was decreased by 3-fold after treatment with HylP. Finally, the external addition of smaller HA oligosaccharides released by HylP enzyme exhibited negligible influence on cell proliferation and invasion. In conclusion, the diminution of extracellular HA by HylP enzyme effectively inhibits both cell proliferation and invasion of aggressive breast cancer cells and suggests the potential as a novel therapeutics." @default.
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• W2897812529 date "2008-05-01" @default.
• W2897812529 modified "2023-09-26" @default.
• W2897812529 title "In vitro studies on anti-cancer effect of Streptococcus pyogenes phage hyaluronidase (HylP) on breast cancer" @default.
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