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- W2897856002 abstract "Abstract A wide range of crops such as bananas and plantains are devastated by plant parasitic nematodes that affect the root system, leading to impaired plant growth and production. In this scenario, the chitinase gene was isolated from the nematode-resistant cultivar of Pisang lilin. The amplified target gene was cloned into pET28a encoding 323 amino acid residues, which shares a high degree of homology with acidic class III chitinase in Musa acuminata, and the product was transformed into E. coli BL21 strain for protein expression. Using affinity chromatography, the recombinant chitinase was purified as a protein of 29 kDa with an optimal activity at pH 7.0. The purified chitinase was capable of degrading the chitin present in the exoskeleton layer of root lesion nematodes (Pratylenchus coffeae) and in the eggshells of root-knot nematodes (Meloidogyne incognita), as well as significantly influencing its development and mortality. Bioassay was performed under in vitro conditions. In root lesion nematodes, 100% mortality was recorded at concentrations of 0.2 µg/mL and 0.15 µg/mL on the 3rd and 5th days, respectively, whereas the egg-hatching rate was inhibited by 100% at concentrations of 0.2 µg/mL and 0.01 µg/mL on the 1st and 5th days, respectively. The results of this study suggest that the PlchiIII gene can be used as a plant-based microbial agent in the biocontrol of nematodes would help in reducing nematode population and increase the yield of banana." @default.
- W2897856002 created "2018-10-26" @default.
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- W2897856002 date "2018-10-01" @default.
- W2897856002 modified "2023-09-24" @default.
- W2897856002 title "Cloning of the defense gene PlchiIII and its potential role in the biocontrol of Pratylenchus coffeae nematodes and Meloidogyne incognita eggs in Musa" @default.
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- W2897856002 doi "https://doi.org/10.1016/j.bcab.2018.10.004" @default.
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