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- W2897865345 endingPage "97" @default.
- W2897865345 startingPage "87" @default.
- W2897865345 abstract "Pseudomonas putida is characterized by a versatile metabolism and stress tolerance traits that allow the bacterium to cope with different environmental conditions. In this work, the mechanisms that allow P. putida KT2440 to grow in the presence of four sole carbon sources (glucose, citrate, ferulic acid, serine) were investigated by RNA sequencing (RNA-seq) and genome-scale metabolic modelling. Transcriptomic data identified uptake systems for the four carbon sources, and candidates were subjected to preliminary experimental characterization by mutant strain growth to test their involvement in substrate assimilation. The OpdH and BenF-like porins were involved in citrate and ferulic acid uptake respectively. The citrate transporter (encoded by PP_0147) and the TctABC system were important for supporting cell growth in citrate; PcaT and VanK were associated with ferulic acid uptake; and the ABC transporter AapJPQM was involved in serine transport. A genome-scale metabolic model of P. putida KT2440 was used to integrate and analyze the transcriptomic data, identifying and confirming the active catabolic pathways for each carbon source. This study reveals novel information about transporters that are essential for understanding bacterial adaptation to different environments." @default.
- W2897865345 created "2018-10-26" @default.
- W2897865345 creator A5003812348 @default.
- W2897865345 creator A5015184122 @default.
- W2897865345 creator A5050504035 @default.
- W2897865345 creator A5050986295 @default.
- W2897865345 creator A5076063242 @default.
- W2897865345 creator A5076738885 @default.
- W2897865345 date "2018-11-22" @default.
- W2897865345 modified "2023-09-26" @default.
- W2897865345 title "Analysis of<i>Pseudomonas putida</i>growth on non‐trivial carbon sources using transcriptomics and genome‐scale modelling" @default.
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