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• W2897894275 abstract "This chapter expounds the single vesicle-vesicle fluorescence resonance energy transfer (FRET) measurement to study the membrane fusion mediated by SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins. The formation of a four-α-helix bundle of SNARE proteins can drive two membranes to a close proximity for fusion. Through single-molecule FRET-based microscopy, the lipid-mixing process at the single-vesicle level can be tracked in real time. This reconstitution system is applicable to study the molecular mechanism of SNAREs during different membrane fusion stages, such as docking, hemifusion, and full fusion. Four main parts are described in this chapter, including SNARE reconstitution, imaging preparation, data collection, and analysis." @default.
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• W2897894275 date "2018-10-14" @default.
• W2897894275 modified "2023-10-16" @default.
• W2897894275 title "Single-Molecule Fluorescence Measurement of SNARE-Mediated Vesicle Fusion" @default.
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• W2897894275 doi "https://doi.org/10.1007/978-1-4939-8760-3_22" @default.
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