Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897895206> ?p ?o ?g. }
- W2897895206 endingPage "16369" @default.
- W2897895206 startingPage "16364" @default.
- W2897895206 abstract "Super-resolution microscopy requires small fluorescent labels. We report the application of genetic code expansion in combination with bioorthogonal click chemistry to label the NR1 domain of the NMDA receptor. We generated NR1 mutants incorporating an unnatural amino acid at various positions in order to attach small organic fluorophores such as Cy5-tetrazine site-specifically to the extracellular domain of the receptor. Mutants were optimized with regard to protein expression, labeling efficiency and receptor functionality as tested by fluorescence microscopy and whole-cell patch clamp. The results show that bioorthogonal click chemistry in combination with small organic dyes is superior to available immunocytochemistry protocols for receptor labeling in live and fixed cells and enables single-molecule sensitive super-resolution microscopy experiments." @default.
- W2897895206 created "2018-10-26" @default.
- W2897895206 creator A5003394753 @default.
- W2897895206 creator A5027466984 @default.
- W2897895206 creator A5052344603 @default.
- W2897895206 creator A5066726616 @default.
- W2897895206 creator A5077791684 @default.
- W2897895206 creator A5082193758 @default.
- W2897895206 creator A5089086027 @default.
- W2897895206 date "2018-11-15" @default.
- W2897895206 modified "2023-10-13" @default.
- W2897895206 title "Bioorthogonal Click Chemistry Enables Site‐specific Fluorescence Labeling of Functional NMDA Receptors for Super‐Resolution Imaging" @default.
- W2897895206 cites W1155097428 @default.
- W2897895206 cites W1606256000 @default.
- W2897895206 cites W1964487937 @default.
- W2897895206 cites W1965698505 @default.
- W2897895206 cites W1974642741 @default.
- W2897895206 cites W1992188171 @default.
- W2897895206 cites W1995754625 @default.
- W2897895206 cites W1998265747 @default.
- W2897895206 cites W2016122189 @default.
- W2897895206 cites W2027516993 @default.
- W2897895206 cites W2030950431 @default.
- W2897895206 cites W2048608623 @default.
- W2897895206 cites W2054989441 @default.
- W2897895206 cites W2059253719 @default.
- W2897895206 cites W2065985186 @default.
- W2897895206 cites W2085040401 @default.
- W2897895206 cites W2088171173 @default.
- W2897895206 cites W2089094370 @default.
- W2897895206 cites W2094654556 @default.
- W2897895206 cites W2106316394 @default.
- W2897895206 cites W2106396641 @default.
- W2897895206 cites W2126579241 @default.
- W2897895206 cites W2140274999 @default.
- W2897895206 cites W2148123458 @default.
- W2897895206 cites W2158271064 @default.
- W2897895206 cites W2160079170 @default.
- W2897895206 cites W2162454135 @default.
- W2897895206 cites W2163414746 @default.
- W2897895206 cites W2172791605 @default.
- W2897895206 cites W2188496389 @default.
- W2897895206 cites W2294307104 @default.
- W2897895206 cites W2298172813 @default.
- W2897895206 cites W2332024781 @default.
- W2897895206 cites W2340986337 @default.
- W2897895206 cites W2507972977 @default.
- W2897895206 cites W2545229506 @default.
- W2897895206 cites W2548801425 @default.
- W2897895206 cites W2548916909 @default.
- W2897895206 cites W2558137622 @default.
- W2897895206 cites W2595114075 @default.
- W2897895206 cites W2597088123 @default.
- W2897895206 cites W2599517595 @default.
- W2897895206 cites W2608923220 @default.
- W2897895206 cites W2724786424 @default.
- W2897895206 cites W2756063862 @default.
- W2897895206 cites W2806800197 @default.
- W2897895206 cites W2884268559 @default.
- W2897895206 cites W4250054944 @default.
- W2897895206 doi "https://doi.org/10.1002/anie.201808951" @default.
- W2897895206 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30347512" @default.
- W2897895206 hasPublicationYear "2018" @default.
- W2897895206 type Work @default.
- W2897895206 sameAs 2897895206 @default.
- W2897895206 citedByCount "39" @default.
- W2897895206 countsByYear W28978952062019 @default.
- W2897895206 countsByYear W28978952062020 @default.
- W2897895206 countsByYear W28978952062021 @default.
- W2897895206 countsByYear W28978952062022 @default.
- W2897895206 countsByYear W28978952062023 @default.
- W2897895206 crossrefType "journal-article" @default.
- W2897895206 hasAuthorship W2897895206A5003394753 @default.
- W2897895206 hasAuthorship W2897895206A5027466984 @default.
- W2897895206 hasAuthorship W2897895206A5052344603 @default.
- W2897895206 hasAuthorship W2897895206A5066726616 @default.
- W2897895206 hasAuthorship W2897895206A5077791684 @default.
- W2897895206 hasAuthorship W2897895206A5082193758 @default.
- W2897895206 hasAuthorship W2897895206A5089086027 @default.
- W2897895206 hasConcept C121332964 @default.
- W2897895206 hasConcept C12554922 @default.
- W2897895206 hasConcept C169274487 @default.
- W2897895206 hasConcept C185592680 @default.
- W2897895206 hasConcept C21951064 @default.
- W2897895206 hasConcept C57477423 @default.
- W2897895206 hasConcept C62520636 @default.
- W2897895206 hasConcept C69610077 @default.
- W2897895206 hasConcept C86803240 @default.
- W2897895206 hasConcept C91881484 @default.
- W2897895206 hasConcept C930959 @default.
- W2897895206 hasConceptScore W2897895206C121332964 @default.
- W2897895206 hasConceptScore W2897895206C12554922 @default.
- W2897895206 hasConceptScore W2897895206C169274487 @default.
- W2897895206 hasConceptScore W2897895206C185592680 @default.
- W2897895206 hasConceptScore W2897895206C21951064 @default.
- W2897895206 hasConceptScore W2897895206C57477423 @default.
- W2897895206 hasConceptScore W2897895206C62520636 @default.
- W2897895206 hasConceptScore W2897895206C69610077 @default.