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- W28979211 abstract "A method of the solid-phase enzymic synthesis of oligoribonucleotides has been suggested. The donor is fixed through its 3'-end on a water-insoluble matrix followed by the stepwise RNA ligase- and T4 polynucleotide kinase-assisted coupling of trinucleoside diphosphates in the 5'-direction. As an example, (pA)6pAox was immobilised on Biogel P-300 hydrazine and the RNA ligase-catalyzed addition of acceptor ApApA to the donor gave (Ap)9 with the 50% yield." @default.
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- W28979211 date "1991-06-01" @default.
- W28979211 modified "2023-09-28" @default.
- W28979211 title "RNA ligase from bacteriophage T4. VIII. Solid phase enzymatic synthesis of oligoribonucleotides" @default.
- W28979211 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1776967" @default.
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