Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897947412> ?p ?o ?g. }
- W2897947412 abstract "Abstract Due to their intimate physical interactions with the environment, surface polysaccharides are critical determinants of fitness for bacteria. Caulobacter crescentus produces a specialized structure at one of its cell poles called the holdfast that enables attachment to surfaces. Previous studies have shown that the holdfast is a carbohydrate-based material and identified a number of genes required for holdfast development. However, incomplete information about its chemical structure, biosynthetic genes and regulatory principles has limited progress in understanding the mechanism of holdfast synthesis. We have leveraged the adhesive properties of the holdfast to perform a saturating screen for genes affecting attachment to cheesecloth over a multi-day time course. Using similarities in the temporal profiles of mutants in a transposon library, we defined discrete clusters of genes with related effects on cheesecloth colonization. Holdfast synthesis, flagellar motility, type IV pilus assembly and smooth lipopolysaccharide (SLPS) production represented key classes of adhesion determinants. Examining these clusters in detail allowed us to predict and experimentally define the functions of multiple uncharacterized genes in both the holdfast and SLPS pathways. In addition, we showed that the pilus and flagellum control holdfast synthesis separately by modulating the holdfast inhibitor hfiA. This study defines a set of genes contributing to adhesion that includes newly discovered genes required for holdfast biosynthesis and attachment. Our data provide evidence that the holdfast contains a complex polysaccharide with at least four monosaccharides in the repeating unit and underscore the central role of cell polarity in mediating attachment of C. crescentus to surfaces. Importance Bacteria routinely encounter biotic and abiotic materials in their surrounding environments, and they often enlist specific behavioral programs to colonize these materials. Adhesion is an early step in colonizing a surface. Caulobacter crescentus produces a structure called the holdfast, which allows this organism to attach to and colonize surfaces. To understand how the holdfast is produced, we performed a genome-wide search for genes that contribute to adhesion by selecting for mutants that could not attach to cheesecloth. We discovered complex interactions between genes that mediate surface contact and genes that contribute to holdfast development. Our genetic selection identified what likely represents a comprehensive set of genes required to generate a holdfast, laying the groundwork for a detailed characterization of the enzymes that build this specialized adhesin." @default.
- W2897947412 created "2018-10-26" @default.
- W2897947412 creator A5037319626 @default.
- W2897947412 creator A5055058443 @default.
- W2897947412 creator A5068647102 @default.
- W2897947412 date "2018-10-18" @default.
- W2897947412 modified "2023-09-23" @default.
- W2897947412 title "A genome-wide analysis of adhesion inCaulobacter crescentusidentifies new regulatory and biosynthetic components for holdfast assembly" @default.
- W2897947412 cites W1495831881 @default.
- W2897947412 cites W1558058576 @default.
- W2897947412 cites W1587696430 @default.
- W2897947412 cites W1828346848 @default.
- W2897947412 cites W1837256871 @default.
- W2897947412 cites W1869272742 @default.
- W2897947412 cites W1893801608 @default.
- W2897947412 cites W1898737760 @default.
- W2897947412 cites W1898780412 @default.
- W2897947412 cites W1919976334 @default.
- W2897947412 cites W1932587884 @default.
- W2897947412 cites W1936644380 @default.
- W2897947412 cites W1954112402 @default.
- W2897947412 cites W1963147033 @default.
- W2897947412 cites W1968701196 @default.
- W2897947412 cites W1971439989 @default.
- W2897947412 cites W1984788101 @default.
- W2897947412 cites W1992236963 @default.
- W2897947412 cites W2005478409 @default.
- W2897947412 cites W2006591980 @default.
- W2897947412 cites W2016411536 @default.
- W2897947412 cites W2027425031 @default.
- W2897947412 cites W2028831558 @default.
- W2897947412 cites W2030705572 @default.
- W2897947412 cites W2030924818 @default.
- W2897947412 cites W2040803239 @default.
- W2897947412 cites W2040912096 @default.
- W2897947412 cites W2060767800 @default.
- W2897947412 cites W2070415963 @default.
- W2897947412 cites W2076071492 @default.
- W2897947412 cites W2081860279 @default.
- W2897947412 cites W2090417696 @default.
- W2897947412 cites W2093358630 @default.
- W2897947412 cites W2099085924 @default.
- W2897947412 cites W2102906662 @default.
- W2897947412 cites W2117319600 @default.
- W2897947412 cites W2131891756 @default.
- W2897947412 cites W2136856188 @default.
- W2897947412 cites W2142038763 @default.
- W2897947412 cites W2147371463 @default.
- W2897947412 cites W2150926065 @default.
- W2897947412 cites W2151436324 @default.
- W2897947412 cites W2153098264 @default.
- W2897947412 cites W2155906662 @default.
- W2897947412 cites W2160765784 @default.
- W2897947412 cites W2170267077 @default.
- W2897947412 cites W2173692600 @default.
- W2897947412 cites W2174380698 @default.
- W2897947412 cites W2308351058 @default.
- W2897947412 cites W2374649359 @default.
- W2897947412 cites W2568577966 @default.
- W2897947412 cites W2724541800 @default.
- W2897947412 cites W2766517818 @default.
- W2897947412 cites W2792727468 @default.
- W2897947412 cites W2804515387 @default.
- W2897947412 cites W3098107916 @default.
- W2897947412 cites W4252095208 @default.
- W2897947412 cites W950515816 @default.
- W2897947412 doi "https://doi.org/10.1101/446781" @default.
- W2897947412 hasPublicationYear "2018" @default.
- W2897947412 type Work @default.
- W2897947412 sameAs 2897947412 @default.
- W2897947412 citedByCount "3" @default.
- W2897947412 countsByYear W28979474122019 @default.
- W2897947412 crossrefType "posted-content" @default.
- W2897947412 hasAuthorship W2897947412A5037319626 @default.
- W2897947412 hasAuthorship W2897947412A5055058443 @default.
- W2897947412 hasAuthorship W2897947412A5068647102 @default.
- W2897947412 hasBestOaLocation W28979474121 @default.
- W2897947412 hasConcept C104317684 @default.
- W2897947412 hasConcept C115811362 @default.
- W2897947412 hasConcept C143065580 @default.
- W2897947412 hasConcept C174832134 @default.
- W2897947412 hasConcept C18903297 @default.
- W2897947412 hasConcept C203075996 @default.
- W2897947412 hasConcept C2778707143 @default.
- W2897947412 hasConcept C2778767192 @default.
- W2897947412 hasConcept C2779594066 @default.
- W2897947412 hasConcept C29537977 @default.
- W2897947412 hasConcept C36857842 @default.
- W2897947412 hasConcept C523546767 @default.
- W2897947412 hasConcept C54355233 @default.
- W2897947412 hasConcept C60987743 @default.
- W2897947412 hasConcept C86803240 @default.
- W2897947412 hasConcept C89423630 @default.
- W2897947412 hasConcept C95444343 @default.
- W2897947412 hasConceptScore W2897947412C104317684 @default.
- W2897947412 hasConceptScore W2897947412C115811362 @default.
- W2897947412 hasConceptScore W2897947412C143065580 @default.
- W2897947412 hasConceptScore W2897947412C174832134 @default.
- W2897947412 hasConceptScore W2897947412C18903297 @default.
- W2897947412 hasConceptScore W2897947412C203075996 @default.