Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897951223> ?p ?o ?g. }
- W2897951223 endingPage "1588" @default.
- W2897951223 startingPage "1581" @default.
- W2897951223 abstract "If a transgene is effectively delivered to a cell, its expression may still be limited by epigenetic mechanisms that silence the transgene. Indeed, once the transgene reaches the nucleus, it may be bound by histone proteins and condensed into heterochromatin or associated with repressor proteins that block transcription. In this study, we sought to enhance transgene expression by adding binding motifs for several different epigenetic enzymes either upstream or downstream of two promoters (CMV and EF1α). Screening these plasmids revealed that luciferase expression was enhanced 10‐fold (10.4 ± 5.8) by the addition of a CCAAT box just upstream of the EF1α promoter to recruit nuclear transcription factor Y (NF‐Y), while inserting a CCCTC‐binding factor (CTCF) motif downstream of the EF1α promoter enhanced expression at least 14‐fold (14.03 ± 6.54). ChIP assays confirmed that NF‐Y and CTCF bound to the motifs that were added to each plasmid, but the presence of NF‐Y and CTCF did not significantly affect the levels of histone acetylation (H3K9ac) or methylation (H3K9me3). Overall, these results show that transgene expression from the EF1α promoter can be significantly increased with motifs that recruit NF‐Y or CTCF. © 2018 American Institute of Chemical Engineers Biotechnol. Prog ., 34:1581–1588, 2018" @default.
- W2897951223 created "2018-10-26" @default.
- W2897951223 creator A5021090743 @default.
- W2897951223 creator A5025512444 @default.
- W2897951223 creator A5032676193 @default.
- W2897951223 creator A5090369207 @default.
- W2897951223 date "2018-10-09" @default.
- W2897951223 modified "2023-09-28" @default.
- W2897951223 title "Enhancement of transgene expression by nuclear transcription factor Y and CCCTC‐binding factor" @default.
- W2897951223 cites W1506343731 @default.
- W2897951223 cites W1774630295 @default.
- W2897951223 cites W1828624188 @default.
- W2897951223 cites W1967822975 @default.
- W2897951223 cites W1971918585 @default.
- W2897951223 cites W1979014200 @default.
- W2897951223 cites W1991208204 @default.
- W2897951223 cites W1992758099 @default.
- W2897951223 cites W1992788889 @default.
- W2897951223 cites W2006620181 @default.
- W2897951223 cites W2015416439 @default.
- W2897951223 cites W2016923780 @default.
- W2897951223 cites W2020256361 @default.
- W2897951223 cites W2022285476 @default.
- W2897951223 cites W2027799462 @default.
- W2897951223 cites W2029722028 @default.
- W2897951223 cites W2031087599 @default.
- W2897951223 cites W2034602099 @default.
- W2897951223 cites W2039564969 @default.
- W2897951223 cites W2039618053 @default.
- W2897951223 cites W2040112493 @default.
- W2897951223 cites W2040365591 @default.
- W2897951223 cites W2041983142 @default.
- W2897951223 cites W2042976701 @default.
- W2897951223 cites W2046842005 @default.
- W2897951223 cites W2059482132 @default.
- W2897951223 cites W2063065954 @default.
- W2897951223 cites W2074132134 @default.
- W2897951223 cites W2083150665 @default.
- W2897951223 cites W2086038224 @default.
- W2897951223 cites W2092293354 @default.
- W2897951223 cites W2096736503 @default.
- W2897951223 cites W2097081350 @default.
- W2897951223 cites W2103948531 @default.
- W2897951223 cites W2105263500 @default.
- W2897951223 cites W2106189584 @default.
- W2897951223 cites W2108691372 @default.
- W2897951223 cites W2110691960 @default.
- W2897951223 cites W2111041465 @default.
- W2897951223 cites W2113470975 @default.
- W2897951223 cites W2114161957 @default.
- W2897951223 cites W2118887202 @default.
- W2897951223 cites W2123318986 @default.
- W2897951223 cites W2125081662 @default.
- W2897951223 cites W2127033140 @default.
- W2897951223 cites W2139195068 @default.
- W2897951223 cites W2144256548 @default.
- W2897951223 cites W2146026647 @default.
- W2897951223 cites W2146259423 @default.
- W2897951223 cites W2152497434 @default.
- W2897951223 cites W2155304372 @default.
- W2897951223 cites W2156936974 @default.
- W2897951223 cites W2162438863 @default.
- W2897951223 cites W2175853567 @default.
- W2897951223 cites W2262543291 @default.
- W2897951223 cites W2279960284 @default.
- W2897951223 cites W2321908728 @default.
- W2897951223 cites W2345720602 @default.
- W2897951223 cites W2418367670 @default.
- W2897951223 cites W2527535582 @default.
- W2897951223 cites W2530088106 @default.
- W2897951223 cites W2558647619 @default.
- W2897951223 cites W4235642564 @default.
- W2897951223 doi "https://doi.org/10.1002/btpr.2712" @default.
- W2897951223 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30294957" @default.
- W2897951223 hasPublicationYear "2018" @default.
- W2897951223 type Work @default.
- W2897951223 sameAs 2897951223 @default.
- W2897951223 citedByCount "7" @default.
- W2897951223 countsByYear W28979512232018 @default.
- W2897951223 countsByYear W28979512232019 @default.
- W2897951223 countsByYear W28979512232020 @default.
- W2897951223 countsByYear W28979512232021 @default.
- W2897951223 crossrefType "journal-article" @default.
- W2897951223 hasAuthorship W2897951223A5021090743 @default.
- W2897951223 hasAuthorship W2897951223A5025512444 @default.
- W2897951223 hasAuthorship W2897951223A5032676193 @default.
- W2897951223 hasAuthorship W2897951223A5090369207 @default.
- W2897951223 hasConcept C101762097 @default.
- W2897951223 hasConcept C102230213 @default.
- W2897951223 hasConcept C104317684 @default.
- W2897951223 hasConcept C111936080 @default.
- W2897951223 hasConcept C138885662 @default.
- W2897951223 hasConcept C150194340 @default.
- W2897951223 hasConcept C153911025 @default.
- W2897951223 hasConcept C158448853 @default.
- W2897951223 hasConcept C179716448 @default.
- W2897951223 hasConcept C179926584 @default.
- W2897951223 hasConcept C2778760011 @default.