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- W2898046132 abstract "In situ bybridization method and immunocytochemical staining assay were used to detect the expressional level of bcl-2 mRNA and protein separately. Then artificially synthesized bcl-2 specific antisense oligodeoxynucleotids were added to in vitro HL-60 culture system at different concentration in order that these antisense oligomers were introduced into cytoplasm of HL-60 cells. It is found that HL-60 cells possessed higher expression of bcl-2 gene both at mRNA and protein level. Expression of bcl-2 mRNA in HL-60 was observed and expression of bcl-2 protein was noticed bcl-2 mRNA specific antisense oligodeoxynucleotid could inhibit the expression of Bcl-2 protein in Hl-60 cells. In future gene therapy research for leukemia, the oncogene bcl-2 may be choiced as a useful target." @default.
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- W2898046132 date "1996-01-01" @default.
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- W2898046132 title "Inhibitive effect of an antisense oligodeoxynucleotid on the expression of bel-2 in HL-60 cells" @default.
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