Matches in SemOpenAlex for { <https://semopenalex.org/work/W2898047543> ?p ?o ?g. }
- W2898047543 endingPage "1802918" @default.
- W2898047543 startingPage "1802918" @default.
- W2898047543 abstract "Secreted proteins determine a range of cellular functionalities correlated with human health and disease progression. Because of cell heterogeneity, it is essential to measure low abundant protein secretions from individual cells to determine single-cell activities. In this study, an integrated platform consisting of smart hydrogel immunosensors for the sensitive detection of single-cell secretions is developed. A single cell and smart hydrogel microparticles are encapsulated within a droplet. After incubation, target secreted proteins from the cell are captured in the smart hydrogel particle for immunoassay. The temperature-induced volume phase transition of the hydrogel biosensor allows the concentration of analytes within the gel matrix to increase, enabling high-sensitivity measurements. Distinct heterogeneity for live cell secretions is determined from 6000 cells within 1 h. This method is tested for low abundant essential secretions, such as interleukin-6, interleukin-8, and monocyte chemoattractant protein-1 secretions of both suspended cells (HL60) and adherent cells (MCF7 and MDA-MB-231). This platform is highly flexible and can be used to simultaneously measure a wide range of clinically relevant cellular secretions; it thus represents a novel tool for precise biological assays." @default.
- W2898047543 created "2018-10-26" @default.
- W2898047543 creator A5004333719 @default.
- W2898047543 creator A5006256656 @default.
- W2898047543 creator A5034837211 @default.
- W2898047543 creator A5042293294 @default.
- W2898047543 creator A5064222660 @default.
- W2898047543 creator A5066513731 @default.
- W2898047543 date "2018-10-17" @default.
- W2898047543 modified "2023-10-15" @default.
- W2898047543 title "Smart Hydrogel Microfluidics for Single-Cell Multiplexed Secretomic Analysis with High Sensitivity" @default.
- W2898047543 cites W1567942562 @default.
- W2898047543 cites W1945392523 @default.
- W2898047543 cites W1970217459 @default.
- W2898047543 cites W1984227596 @default.
- W2898047543 cites W2004162643 @default.
- W2898047543 cites W2014993834 @default.
- W2898047543 cites W2015554529 @default.
- W2898047543 cites W2016541161 @default.
- W2898047543 cites W2032805499 @default.
- W2898047543 cites W2037493777 @default.
- W2898047543 cites W2038569827 @default.
- W2898047543 cites W2043900543 @default.
- W2898047543 cites W2050893597 @default.
- W2898047543 cites W2055561436 @default.
- W2898047543 cites W2055669282 @default.
- W2898047543 cites W2062100461 @default.
- W2898047543 cites W2070727822 @default.
- W2898047543 cites W2073307618 @default.
- W2898047543 cites W2088941669 @default.
- W2898047543 cites W2092456329 @default.
- W2898047543 cites W2102946765 @default.
- W2898047543 cites W2114385847 @default.
- W2898047543 cites W2122309838 @default.
- W2898047543 cites W2122359223 @default.
- W2898047543 cites W2129297473 @default.
- W2898047543 cites W2135937351 @default.
- W2898047543 cites W2146207791 @default.
- W2898047543 cites W2158778815 @default.
- W2898047543 cites W2167279371 @default.
- W2898047543 cites W2168204150 @default.
- W2898047543 cites W2297436952 @default.
- W2898047543 cites W2300724465 @default.
- W2898047543 cites W2415256212 @default.
- W2898047543 cites W2418970963 @default.
- W2898047543 cites W2549451374 @default.
- W2898047543 cites W2564176045 @default.
- W2898047543 cites W2747270418 @default.
- W2898047543 cites W2750595332 @default.
- W2898047543 cites W2805898794 @default.
- W2898047543 cites W4240972026 @default.
- W2898047543 doi "https://doi.org/10.1002/smll.201802918" @default.
- W2898047543 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30334375" @default.
- W2898047543 hasPublicationYear "2018" @default.
- W2898047543 type Work @default.
- W2898047543 sameAs 2898047543 @default.
- W2898047543 citedByCount "48" @default.
- W2898047543 countsByYear W28980475432019 @default.
- W2898047543 countsByYear W28980475432020 @default.
- W2898047543 countsByYear W28980475432021 @default.
- W2898047543 countsByYear W28980475432022 @default.
- W2898047543 countsByYear W28980475432023 @default.
- W2898047543 crossrefType "journal-article" @default.
- W2898047543 hasAuthorship W2898047543A5004333719 @default.
- W2898047543 hasAuthorship W2898047543A5006256656 @default.
- W2898047543 hasAuthorship W2898047543A5034837211 @default.
- W2898047543 hasAuthorship W2898047543A5042293294 @default.
- W2898047543 hasAuthorship W2898047543A5064222660 @default.
- W2898047543 hasAuthorship W2898047543A5066513731 @default.
- W2898047543 hasConcept C10390740 @default.
- W2898047543 hasConcept C108586683 @default.
- W2898047543 hasConcept C12554922 @default.
- W2898047543 hasConcept C1491633281 @default.
- W2898047543 hasConcept C160756335 @default.
- W2898047543 hasConcept C171250308 @default.
- W2898047543 hasConcept C178790620 @default.
- W2898047543 hasConcept C185592680 @default.
- W2898047543 hasConcept C192562407 @default.
- W2898047543 hasConcept C43617362 @default.
- W2898047543 hasConcept C55493867 @default.
- W2898047543 hasConcept C8673954 @default.
- W2898047543 hasConcept C86803240 @default.
- W2898047543 hasConcept C95444343 @default.
- W2898047543 hasConceptScore W2898047543C10390740 @default.
- W2898047543 hasConceptScore W2898047543C108586683 @default.
- W2898047543 hasConceptScore W2898047543C12554922 @default.
- W2898047543 hasConceptScore W2898047543C1491633281 @default.
- W2898047543 hasConceptScore W2898047543C160756335 @default.
- W2898047543 hasConceptScore W2898047543C171250308 @default.
- W2898047543 hasConceptScore W2898047543C178790620 @default.
- W2898047543 hasConceptScore W2898047543C185592680 @default.
- W2898047543 hasConceptScore W2898047543C192562407 @default.
- W2898047543 hasConceptScore W2898047543C43617362 @default.
- W2898047543 hasConceptScore W2898047543C55493867 @default.
- W2898047543 hasConceptScore W2898047543C8673954 @default.
- W2898047543 hasConceptScore W2898047543C86803240 @default.
- W2898047543 hasConceptScore W2898047543C95444343 @default.
- W2898047543 hasFunder F4320320709 @default.