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- W2898052040 abstract "Copyright information: Taken from Inactivation of NMD increases viability of nonsense mutants in http://www.biomedcentral.com/1471-2199/8/71BMC Molecular Biology 2007;8():71-71.Published online 16 Aug 2007PMCID:PMC2039749. mature forms of . Total RNA was isolated from strain 3v-D1658 (pRS315/) and its derivates (pRS315/) transformed with pRS316 and pRS316/plasmids, designated as (-) and (+), respectively. The Northern blots were hybridized with radiolabeled probe. The precursor/mature ratio in wild-type strain was set as 1.0. . The fold increase in precursor/mature mRNA accumulation measured in the same strains as in panel A are represented relative to such in wild-type strain. . The same transformants as in panel A were tested by plating 10, 10and 10serial dilutions of overnight cultures (to ) on synthetic complete plates lacking adenine and incubated 5 days at 25°C. The same serially diluted cultures were also spotted on plates lacking leucine and uracil (-L -U) to estimate the total number of cells analyzed. . Norhern blots prepared with total RNA from the same transformants as in panel A were hybridized with radiolabeled probes, detecting , and scR1 mRNA (scR1 was used as a control). eRF1 and eRF3 protein levels in the same transformants were analyzed by western blot. Tubulin was used as a loading control. WT – wild type, 102 – (nonsense), 107 – (nonsense)." @default.
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- W2898052040 date "2011-01-01" @default.
- W2898052040 modified "2023-09-23" @default.
- W2898052040 title "Inactivation of NMD increases viability of nonsense mutants in -4" @default.
- W2898052040 doi "https://doi.org/10.6084/m9.figshare.19371" @default.
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