Matches in SemOpenAlex for { <https://semopenalex.org/work/W2898055312> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2898055312 abstract "KIF3A is a motor protein that is essential for antegrade intraflagellar transport and the maintenance of primary cilia. We have previously shown that kidney-specific excision of Kif3a mediated by Ksp/Cre transgenic mice leads to renal cyst formation by postnatal day (P)5 (Lin et al, PNAS 2003;100:5286-91). The cyst epithelial cells lack primary cilia, but it is not clear if the loss of cilia precedes cyst formation. To better characterize the time course of the mutant phenotype, we produced Pkhd1/Cre transgenic mice that express Cre recombinase under the control of 4.7 kb of the mouse Pkhd1 (ARPKD) promoter. Pkhd1/Cre mice were crossed with R26R mice that express lacZ after Cre/loxP recombination. X-gal staining of bitransgenic Pkhd1/Cre;R26R progeny at P1 revealed that lacZ was specifically expressed in tubular epithelial cells in the renal collecting ducts, intrahepatic bile ducts, and epididymis. No lacZ expression was observed in embryos at E12.5. Pkhd1/Cre mice were crossed with mice carrying a floxed allele of Kif3a and the R26R reporter gene. Analysis of Pkhd1/Cre;Kif3aflox/−;R262R progeny at P7 showed that the kidneys were morphologically normal and that lacZ was expressed in noncystic collecting ducts. Antibody staining for acetylated tubulin showed that primary cilia were absent in the lacZ-positive collecting duct cells in the Kif3a mutant mice but were present in control littermates. Kidney cysts were observed in mutant mice at P14 and progressively increased in size and number at P21, P35, and P56. All cysts were derived from the collecting ducts and were lined by lacZ-positive cyst epithelial cells. The cyst epithelial cells lacked primary cilia and showed apical localization of ZO-1 and basolateral localization of aquaporin 3. Staining for PCNA showed that cyst epithelial cells had higher rates of cell proliferation compared with wild-type collecting duct cells. No morphologic abnormalities were detected in the livers of Kif3a mutant mice. In conclusion, the formation of kidney cysts in Kif3a mutant mice is directly due to inactivation of Kif3a, which produces a loss of renal cilia that precedes tubular dilatation. Pkhd1/Cre transgenic mice should be useful for tissue-specific targeting of other ciliary genes in the kidney, liver, and epididymis." @default.
- W2898055312 created "2018-10-26" @default.
- W2898055312 creator A5039063866 @default.
- W2898055312 creator A5047057823 @default.
- W2898055312 creator A5071449684 @default.
- W2898055312 creator A5083795868 @default.
- W2898055312 date "2007-01-01" @default.
- W2898055312 modified "2023-09-27" @default.
- W2898055312 title "352 LOSS OF PRIMARY CILIA PRECEDES KIDNEY CYST FORMATION IN KIF3A KNOCKOUT MICE." @default.
- W2898055312 hasPublicationYear "2007" @default.
- W2898055312 type Work @default.
- W2898055312 sameAs 2898055312 @default.
- W2898055312 citedByCount "0" @default.
- W2898055312 crossrefType "journal-article" @default.
- W2898055312 hasAuthorship W2898055312A5039063866 @default.
- W2898055312 hasAuthorship W2898055312A5047057823 @default.
- W2898055312 hasAuthorship W2898055312A5071449684 @default.
- W2898055312 hasAuthorship W2898055312A5083795868 @default.
- W2898055312 hasConcept C100094513 @default.
- W2898055312 hasConcept C102230213 @default.
- W2898055312 hasConcept C104317684 @default.
- W2898055312 hasConcept C125929170 @default.
- W2898055312 hasConcept C134018914 @default.
- W2898055312 hasConcept C141035611 @default.
- W2898055312 hasConcept C142724271 @default.
- W2898055312 hasConcept C143065580 @default.
- W2898055312 hasConcept C153911025 @default.
- W2898055312 hasConcept C195267511 @default.
- W2898055312 hasConcept C2780091579 @default.
- W2898055312 hasConcept C37590093 @default.
- W2898055312 hasConcept C54355233 @default.
- W2898055312 hasConcept C71924100 @default.
- W2898055312 hasConcept C86803240 @default.
- W2898055312 hasConcept C95444343 @default.
- W2898055312 hasConceptScore W2898055312C100094513 @default.
- W2898055312 hasConceptScore W2898055312C102230213 @default.
- W2898055312 hasConceptScore W2898055312C104317684 @default.
- W2898055312 hasConceptScore W2898055312C125929170 @default.
- W2898055312 hasConceptScore W2898055312C134018914 @default.
- W2898055312 hasConceptScore W2898055312C141035611 @default.
- W2898055312 hasConceptScore W2898055312C142724271 @default.
- W2898055312 hasConceptScore W2898055312C143065580 @default.
- W2898055312 hasConceptScore W2898055312C153911025 @default.
- W2898055312 hasConceptScore W2898055312C195267511 @default.
- W2898055312 hasConceptScore W2898055312C2780091579 @default.
- W2898055312 hasConceptScore W2898055312C37590093 @default.
- W2898055312 hasConceptScore W2898055312C54355233 @default.
- W2898055312 hasConceptScore W2898055312C71924100 @default.
- W2898055312 hasConceptScore W2898055312C86803240 @default.
- W2898055312 hasConceptScore W2898055312C95444343 @default.
- W2898055312 hasIssue "1" @default.
- W2898055312 hasLocation W28980553121 @default.
- W2898055312 hasOpenAccess W2898055312 @default.
- W2898055312 hasPrimaryLocation W28980553121 @default.
- W2898055312 hasRelatedWork W171602239 @default.
- W2898055312 hasRelatedWork W1801310573 @default.
- W2898055312 hasRelatedWork W1808273273 @default.
- W2898055312 hasRelatedWork W1973347338 @default.
- W2898055312 hasRelatedWork W1994350846 @default.
- W2898055312 hasRelatedWork W1997843745 @default.
- W2898055312 hasRelatedWork W2040519911 @default.
- W2898055312 hasRelatedWork W2080489534 @default.
- W2898055312 hasRelatedWork W2081728620 @default.
- W2898055312 hasRelatedWork W2093260892 @default.
- W2898055312 hasRelatedWork W2120506431 @default.
- W2898055312 hasRelatedWork W2126323165 @default.
- W2898055312 hasRelatedWork W2136285781 @default.
- W2898055312 hasRelatedWork W2148303395 @default.
- W2898055312 hasRelatedWork W2151482937 @default.
- W2898055312 hasRelatedWork W2156495412 @default.
- W2898055312 hasRelatedWork W2281597736 @default.
- W2898055312 hasRelatedWork W2334178457 @default.
- W2898055312 hasRelatedWork W2552622797 @default.
- W2898055312 hasRelatedWork W2804577493 @default.
- W2898055312 hasVolume "55" @default.
- W2898055312 isParatext "false" @default.
- W2898055312 isRetracted "false" @default.
- W2898055312 magId "2898055312" @default.
- W2898055312 workType "article" @default.