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- W2898223185 abstract "The studies of low-temperature immobilization of bound water in Antarctic lichenized fungus Turgidosculum complicatulum were performed using 1H NMR and DSC over a wide range of thallus hydration. 1H NMR free induction decays were decomposed into a solid component well described by the Gaussian function and two exponentially decaying components coming from a tightly bound water and from a loosely bound water fraction. 1H NMR spectra revealed one averaged mobile proton signal component. 1H NMR measurements recorded in time and in frequency domain suggest the non-cooperative bound water immobilization in T. complicatulum thallus. The threshold of the hydration level estimated by 1H NMR analysis at which the cooperative bound water freezing was detected was Δm/m0 ≈ 0.39, whereas for DSC analysis was equal to Δm/m0 = 0.375. Main ice melting estimated from DSC measurements for zero hydration level of the sample starts at tm = –(19.29 ± 1.19)°C. However, DSC melting peak shows a composed form being a superposition of the main narrow peak (presumably melting of mycobiont areas) and a broad low-temperature shoulder (presumably melting of isolated photobiont cells). DSC traces recorded after two-hour incubation of T. complicatulum thallus at –20 °C suggest much lower threshold level of hydration at which the ice formation occurs (Δm/m0 = 0.0842). Presumably it is a result of diffusion induced migration of separated water molecules to ice microcrystallites already present in thallus, but still beyond the calorimeter resolution." @default.
- W2898223185 created "2018-11-02" @default.
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- W2898223185 date "2019-01-01" @default.
- W2898223185 modified "2023-09-30" @default.
- W2898223185 title "Low-temperature immobilization of water in Antarctic Turgidosculum complicatulum and in Prasiola crispa. Part I. Turgidosculum complicatulum" @default.
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- W2898223185 doi "https://doi.org/10.1016/j.colsurfb.2018.10.059" @default.
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