FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2898383502> ?p ?o ?g. }

• W2898383502 endingPage "L57" @default.
• W2898383502 startingPage "L45" @default.
• W2898383502 abstract "Bitter taste receptors (T2Rs) are a group of 25 chemosensory receptors expressed at significant levels in the human airways. In human airways, bitter taste receptor 14 (T2R14)-mediated physiological response in ameliorating obstructive airway disorders is an active area of investigation. Therefore, understanding various factors regulating the structure and function of T2R14 will be beneficial. We hypothesize that membrane lipids like cholesterol play a regulatory role in T2R14 signaling in airway cells. We confirmed the expression and signaling of T2R14 in primary human airway smooth muscle (HASM) cells and the human airway epithelial cell line (NuLi-1) using immunoblot analysis and intracellular calcium concentration mobilization experiments, respectively. Next, T2R14 signaling was examined in membrane cholesterol-altered environments by methyl-β-cyclodextrin or cholesterol oxidase treatments. In the cells analyzed, cholesterol depletion affected the agonist-induced T2R14 signaling, and cholesterol replenishment rescued its efficacy. An alternative approach for cholesterol depletion (with cholesterol oxidase pretreatment) also negatively affected the agonist potency at T2R14 in HASM cells. To understand the molecular mechanism of interaction between cholesterol and T2R14, we used site-directed mutagenesis coupled with functional assays and examined the role of putative cholesterol-binding motifs (CRAC and CARC) in T2R14. Functional characterization of wild-type and mutant T2R14 receptors suggests that amino acid residues K110, F236, and L239 are crucial in T2R14-cholesterol functional interaction. In conclusion, our results show that cholesterol influences the T2R14 signaling efficacy by forming direct interactions with the receptor and consequently plays a regulatory role in T2R14-mediated signaling in human airway cells." @default.
• W2898383502 created "2018-11-02" @default.
• W2898383502 creator A5001516919 @default.
• W2898383502 creator A5075051998 @default.
• W2898383502 creator A5082452640 @default.
• W2898383502 date "2019-01-01" @default.
• W2898383502 modified "2023-10-11" @default.
• W2898383502 title "Cholesterol modulates the signaling of chemosensory bitter taste receptor T2R14 in human airway cells" @default.
• W2898383502 cites W1513059802 @default.
• W2898383502 cites W1551436342 @default.
• W2898383502 cites W1601065938 @default.
• W2898383502 cites W1601195737 @default.
• W2898383502 cites W1788540485 @default.
• W2898383502 cites W1797076797 @default.
• W2898383502 cites W1969201146 @default.
• W2898383502 cites W1975557054 @default.
• W2898383502 cites W1979166300 @default.
• W2898383502 cites W1987970138 @default.
• W2898383502 cites W1997060256 @default.
• W2898383502 cites W2002485337 @default.
• W2898383502 cites W2004077287 @default.
• W2898383502 cites W2010122297 @default.
• W2898383502 cites W2019406534 @default.
• W2898383502 cites W2023286308 @default.
• W2898383502 cites W2026413548 @default.
• W2898383502 cites W2034866708 @default.
• W2898383502 cites W2036927671 @default.
• W2898383502 cites W2039399096 @default.
• W2898383502 cites W2043864366 @default.
• W2898383502 cites W2046241020 @default.
• W2898383502 cites W204986894 @default.
• W2898383502 cites W2060464351 @default.
• W2898383502 cites W2065886456 @default.
• W2898383502 cites W2072917759 @default.
• W2898383502 cites W2078658298 @default.
• W2898383502 cites W2081606553 @default.
• W2898383502 cites W2083725367 @default.
• W2898383502 cites W2085525100 @default.
• W2898383502 cites W2086424624 @default.
• W2898383502 cites W2091179696 @default.
• W2898383502 cites W2094087197 @default.
• W2898383502 cites W2094770888 @default.
• W2898383502 cites W2096480261 @default.
• W2898383502 cites W2104477830 @default.
• W2898383502 cites W2110880203 @default.
• W2898383502 cites W2110979251 @default.
• W2898383502 cites W2111580238 @default.
• W2898383502 cites W2129361753 @default.
• W2898383502 cites W2133302347 @default.
• W2898383502 cites W2133402952 @default.
• W2898383502 cites W2133940565 @default.
• W2898383502 cites W2137263528 @default.
• W2898383502 cites W2143614476 @default.
• W2898383502 cites W2167813127 @default.
• W2898383502 cites W2169702025 @default.
• W2898383502 cites W2198457549 @default.
• W2898383502 cites W2305146627 @default.
• W2898383502 cites W2341038434 @default.
• W2898383502 cites W2404001249 @default.
• W2898383502 cites W2412283747 @default.
• W2898383502 cites W2565101023 @default.
• W2898383502 cites W2569296714 @default.
• W2898383502 cites W2575290394 @default.
• W2898383502 cites W2588121196 @default.
• W2898383502 cites W2595224182 @default.
• W2898383502 cites W2604798577 @default.
• W2898383502 cites W2605251370 @default.
• W2898383502 cites W2622390452 @default.
• W2898383502 cites W2734763715 @default.
• W2898383502 cites W2734977949 @default.
• W2898383502 cites W2789842168 @default.
• W2898383502 cites W2792532789 @default.
• W2898383502 cites W2799363314 @default.
• W2898383502 cites W2803969691 @default.
• W2898383502 cites W2884881998 @default.
• W2898383502 cites W3109962280 @default.
• W2898383502 doi "https://doi.org/10.1152/ajplung.00169.2018" @default.
• W2898383502 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30358435" @default.
• W2898383502 hasPublicationYear "2019" @default.
• W2898383502 type Work @default.
• W2898383502 sameAs 2898383502 @default.
• W2898383502 citedByCount "11" @default.
• W2898383502 countsByYear W28983835022019 @default.
• W2898383502 countsByYear W28983835022020 @default.
• W2898383502 countsByYear W28983835022021 @default.
• W2898383502 countsByYear W28983835022022 @default.
• W2898383502 countsByYear W28983835022023 @default.
• W2898383502 crossrefType "journal-article" @default.
• W2898383502 hasAuthorship W2898383502A5001516919 @default.
• W2898383502 hasAuthorship W2898383502A5075051998 @default.
• W2898383502 hasAuthorship W2898383502A5082452640 @default.
• W2898383502 hasBestOaLocation W28983835021 @default.
• W2898383502 hasConcept C135285700 @default.
• W2898383502 hasConcept C170493617 @default.
• W2898383502 hasConcept C2778163477 @default.
• W2898383502 hasConcept C2778938600 @default.
• W2898383502 hasConcept C55493867 @default.
• W2898383502 hasConcept C62478195 @default.

• next