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- W2898414344 endingPage "1881" @default.
- W2898414344 startingPage "1867" @default.
- W2898414344 abstract "The eukaryotic genome is divided into chromosomal domains of heterochromatin and euchromatin. Transcriptionally silent heterochromatin is found at subtelomeric regions, leading to the telomeric position effect (TPE) in yeast, fly, and human. Heterochromatin generally initiates and spreads from defined loci, and diverse mechanisms prevent the ectopic spread of heterochromatin into euchromatin. Here, we overexpressed the silencing factor Sir3 at varying levels in yeast and found that Sir3 spreads into extended silent domains (ESDs), eventually reaching saturation at subtelomeres. We observed the spread of Sir3 into subtelomeric domains associated with specific histone marks in wild-type cells, and stopping at zones of histone mark transitions including H3K79 trimethylation levels. Our study shows that the conserved H3K79 methyltransferase Dot1 is essential in restricting Sir3 spread beyond ESDs, thus ensuring viability upon overexpression of Sir3. Last, our analyses of published data demonstrate how ESDs unveil uncharacterized discrete domains isolating structural and functional subtelomeric features from the rest of the genome. Our work offers a new approach on how to separate subtelomeres from the core chromosome." @default.
- W2898414344 created "2018-11-02" @default.
- W2898414344 creator A5001620655 @default.
- W2898414344 creator A5012775306 @default.
- W2898414344 creator A5026926384 @default.
- W2898414344 creator A5030585717 @default.
- W2898414344 creator A5040845783 @default.
- W2898414344 creator A5061414099 @default.
- W2898414344 creator A5067360189 @default.
- W2898414344 date "2018-10-24" @default.
- W2898414344 modified "2023-10-16" @default.
- W2898414344 title "Expanding heterochromatin reveals discrete subtelomeric domains delimited by chromatin landscape transitions" @default.
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