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- W2898457941 abstract "Abstract The recent outbreaks of Zika virus (ZIKV) infection represent a public health challenge. Rapid, cost‐effective, and reliable diagnostic tools for ZIKV detection at the point of care (POC) are highly desirable, especially for resource‐limited nations. To address the need, we have developed an integrated device to achieve sample‐to‐answer ZIKV detection. The device features innovative ball‐based valves enabling the storage and sequential delivery of reagents for virus lysis and a paper‐based unit for RNA enrichment and purification. The paper unit is placed in a commercially available coffee mug that provides a constant temperature for reverse transcription loop‐mediated isothermal amplification (RT‐LAMP), followed by colorimetric detection by naked eye or a cellphone camera. Using the device, we demonstrated the reproducible detection of ZIKV in human urine and saliva samples." @default.
- W2898457941 created "2018-11-02" @default.
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- W2898457941 date "2018-11-27" @default.
- W2898457941 modified "2023-10-15" @default.
- W2898457941 title "Valve‐Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection" @default.
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- W2898457941 doi "https://doi.org/10.1002/anie.201809993" @default.
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