FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2898536632> ?p ?o ?g. }

• W2898536632 endingPage "3886" @default.
• W2898536632 startingPage "3877" @default.
• W2898536632 abstract "DNA methylation is an important way of gene regulation. The variety of methods for DNA methylation analysis based on chemical modification or enzyme digestion has been proposed. However, DNA is able to undergo transformations under physical power. Here, we report that the cytosine methylation in CpG dinucleotides determines the difference in fragmentation rate of methylated and unmethylated DNA under sonication. We found that at the beginning of sonication, methylated DNAs are degraded faster than unmethylated one, and the difference in fragmentation degree can be evaluated with high reliability by quantitative polymerase chain reaction (qPCR). The optimal parameters that provide the greatest difference in amount of amplifiable DNA targets corresponding to fragmentation degree are the following: moderate amplicon size (about 150–250 bp), medium CpG sparseness (one CpG dinucleotide per ∼12–14 nucleotides of the chain), and short sonication time (less than 5 min). Along with CpG, the CpA and CpT contents of amplified regions should be taken into account for proper DNA fragmentation by ultrasound as well. The obtained data could be used for elaboration of a method for comparative methylation testing, when there is no need to detect methylation of certain CpG dinucleotides. This method will be simple (can be used by any technician familiar with PCR), low cost (no need to use an expensive reagents), and fast (only brief DNA sonication and conventional qPCR are carried out).Communicated by Ramaswamy H. Sarma" @default.
• W2898536632 created "2018-11-02" @default.
• W2898536632 creator A5017673895 @default.
• W2898536632 creator A5040489829 @default.
• W2898536632 creator A5055300222 @default.
• W2898536632 date "2018-11-25" @default.
• W2898536632 modified "2023-10-14" @default.
• W2898536632 title "The influence of CpG (5′-d(CpG)-3′ dinucleotides) methylation on ultrasonic DNA fragmentation" @default.
• W2898536632 cites W1508357304 @default.
• W2898536632 cites W1899099918 @default.
• W2898536632 cites W1964797108 @default.
• W2898536632 cites W1967413924 @default.
• W2898536632 cites W1967824604 @default.
• W2898536632 cites W1981528345 @default.
• W2898536632 cites W1987557775 @default.
• W2898536632 cites W1998330439 @default.
• W2898536632 cites W2006334069 @default.
• W2898536632 cites W2010192873 @default.
• W2898536632 cites W2017668896 @default.
• W2898536632 cites W2018604728 @default.
• W2898536632 cites W2026674035 @default.
• W2898536632 cites W2033072260 @default.
• W2898536632 cites W2033916729 @default.
• W2898536632 cites W2043235260 @default.
• W2898536632 cites W2053218599 @default.
• W2898536632 cites W2056360984 @default.
• W2898536632 cites W2059248731 @default.
• W2898536632 cites W2069361073 @default.
• W2898536632 cites W2085338556 @default.
• W2898536632 cites W2093836293 @default.
• W2898536632 cites W2107776519 @default.
• W2898536632 cites W2111584908 @default.
• W2898536632 cites W2113821020 @default.
• W2898536632 cites W2114970231 @default.
• W2898536632 cites W2117125696 @default.
• W2898536632 cites W2141119617 @default.
• W2898536632 cites W2147049379 @default.
• W2898536632 cites W2149562978 @default.
• W2898536632 cites W2171476571 @default.
• W2898536632 cites W2195676082 @default.
• W2898536632 cites W2231664225 @default.
• W2898536632 cites W2414449891 @default.
• W2898536632 cites W2512392141 @default.
• W2898536632 cites W2592303453 @default.
• W2898536632 cites W2623524681 @default.
• W2898536632 cites W2782561013 @default.
• W2898536632 cites W2886223143 @default.
• W2898536632 cites W2887483280 @default.
• W2898536632 cites W4239093244 @default.
• W2898536632 doi "https://doi.org/10.1080/07391102.2018.1533888" @default.
• W2898536632 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30351231" @default.
• W2898536632 hasPublicationYear "2018" @default.
• W2898536632 type Work @default.
• W2898536632 sameAs 2898536632 @default.
• W2898536632 citedByCount "2" @default.
• W2898536632 countsByYear W28985366322020 @default.
• W2898536632 crossrefType "journal-article" @default.
• W2898536632 hasAuthorship W2898536632A5017673895 @default.
• W2898536632 hasAuthorship W2898536632A5040489829 @default.
• W2898536632 hasAuthorship W2898536632A5055300222 @default.
• W2898536632 hasConcept C104317684 @default.
• W2898536632 hasConcept C104628117 @default.
• W2898536632 hasConcept C140173407 @default.
• W2898536632 hasConcept C150194340 @default.
• W2898536632 hasConcept C153911025 @default.
• W2898536632 hasConcept C185592680 @default.
• W2898536632 hasConcept C18903297 @default.
• W2898536632 hasConcept C190283241 @default.
• W2898536632 hasConcept C190727270 @default.
• W2898536632 hasConcept C191015642 @default.
• W2898536632 hasConcept C31573885 @default.
• W2898536632 hasConcept C33288867 @default.
• W2898536632 hasConcept C43617362 @default.
• W2898536632 hasConcept C43759708 @default.
• W2898536632 hasConcept C49105822 @default.
• W2898536632 hasConcept C552990157 @default.
• W2898536632 hasConcept C55493867 @default.
• W2898536632 hasConcept C70721500 @default.
• W2898536632 hasConcept C8185291 @default.
• W2898536632 hasConcept C86803240 @default.
• W2898536632 hasConceptScore W2898536632C104317684 @default.
• W2898536632 hasConceptScore W2898536632C104628117 @default.
• W2898536632 hasConceptScore W2898536632C140173407 @default.
• W2898536632 hasConceptScore W2898536632C150194340 @default.
• W2898536632 hasConceptScore W2898536632C153911025 @default.
• W2898536632 hasConceptScore W2898536632C185592680 @default.
• W2898536632 hasConceptScore W2898536632C18903297 @default.
• W2898536632 hasConceptScore W2898536632C190283241 @default.
• W2898536632 hasConceptScore W2898536632C190727270 @default.
• W2898536632 hasConceptScore W2898536632C191015642 @default.
• W2898536632 hasConceptScore W2898536632C31573885 @default.
• W2898536632 hasConceptScore W2898536632C33288867 @default.
• W2898536632 hasConceptScore W2898536632C43617362 @default.
• W2898536632 hasConceptScore W2898536632C43759708 @default.
• W2898536632 hasConceptScore W2898536632C49105822 @default.
• W2898536632 hasConceptScore W2898536632C552990157 @default.
• W2898536632 hasConceptScore W2898536632C55493867 @default.
• W2898536632 hasConceptScore W2898536632C70721500 @default.

• next