FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2898565265> ?p ?o ?g. }

• W2898565265 endingPage "96" @default.
• W2898565265 startingPage "82" @default.
• W2898565265 abstract "A domain of protein RS1 (<i>RSC1A1</i>) called RS1-Reg down-regulates the plasma membrane abundance of Na⁺-d-glucose cotransporter SGLT1 by blocking the exocytotic pathway at the <i>trans</i>-Golgi. This effect is blunted by intracellular glucose but prevails when serine in a QSP (Gln-Ser-Pro) motif is replaced by glutamate [RS1-Reg(S20E)]. RS1-Reg binds to ornithine decarboxylase (ODC) and inhibits ODC in a glucose-dependent manner. Because the ODC inhibitor difluoromethylornithine (DFMO) acts like RS1-Reg(S20E), and DFMO and RS1-Reg(S20E) are not cumulative, we raised the hypothesis that RS1-Reg(S20E) down-regulates the exocytotic pathway of SGLT1 at the <i>trans</i>-Golgi by inhibiting ODC. We investigated whether QEP down-regulates human SGLT1 (hSGLT1) like hRS1-Reg(S20E) and whether human Na⁺-d-glucose cotransporter hSGLT2 and the human glucose sensor hSGLT3 are also addressed. We expressed hSGLT1, hSGLT1 linked to yellow fluorescent protein (hSGLT1-YFP), hSGLT2-YFP and hSGLT3-YFP in oocytes of <i>Xenopus laevis</i>, injected hRS1-Reg(S20E), QEP, DFMO, and/or <i>α</i>-methyl-d-glucopyranoside (AMG), and measured AMG uptake, glucose-induced currents, and plasma membrane-associated fluorescence after 1 hour. We also performed in vitro AMG uptake measurements into small intestinal mucosa of mice and human. The data indicate that QEP down-regulates the exocytotic pathway of SGLT1 similar to hRS1-Reg(S20E). Our results suggests that both peptides also down-regulate hSGLT2 and hSGLT3 via the same pathway. Thirty minutes after application of 5 mM QEP in the presence of 5 mM d-glucose, hSGLT1-mediated AMG uptake into small intestinal mucosa was decreased by 40% to 50%. Thus oral application of QEP in a formulation that optimizes uptake into enterocytes but prevents entry into the blood is proposed as novel antidiabetic therapy." @default.
• W2898565265 created "2018-11-02" @default.
• W2898565265 creator A5007766006 @default.
• W2898565265 creator A5011521949 @default.
• W2898565265 creator A5011893460 @default.
• W2898565265 creator A5018462164 @default.
• W2898565265 creator A5021026258 @default.
• W2898565265 creator A5064438527 @default.
• W2898565265 creator A5083880434 @default.
• W2898565265 date "2018-10-24" @default.
• W2898565265 modified "2023-10-14" @default.
• W2898565265 title "A Modified Tripeptide Motif of RS1 (<i>RSC1A1</i>) Down-Regulates Exocytotic Pathways of Human Na⁺-d-glucose Cotransporters SGLT1, SGLT2, and Glucose Sensor SGLT3 in the Presence of Glucose" @default.
• W2898565265 cites W1504507024 @default.
• W2898565265 cites W1973975252 @default.
• W2898565265 cites W1973997215 @default.
• W2898565265 cites W1979283687 @default.
• W2898565265 cites W1982531470 @default.
• W2898565265 cites W1986656184 @default.
• W2898565265 cites W1991407392 @default.
• W2898565265 cites W1993025869 @default.
• W2898565265 cites W2006053576 @default.
• W2898565265 cites W2013448178 @default.
• W2898565265 cites W2021126739 @default.
• W2898565265 cites W2037198044 @default.
• W2898565265 cites W2046967221 @default.
• W2898565265 cites W2049081706 @default.
• W2898565265 cites W2049127609 @default.
• W2898565265 cites W2058969403 @default.
• W2898565265 cites W2067898969 @default.
• W2898565265 cites W2086812198 @default.
• W2898565265 cites W2093069407 @default.
• W2898565265 cites W2112043952 @default.
• W2898565265 cites W2115764086 @default.
• W2898565265 cites W2133469996 @default.
• W2898565265 cites W2139636230 @default.
• W2898565265 cites W2141228342 @default.
• W2898565265 cites W2142396748 @default.
• W2898565265 cites W2146343227 @default.
• W2898565265 cites W2147987170 @default.
• W2898565265 cites W2157538794 @default.
• W2898565265 cites W2161645265 @default.
• W2898565265 cites W2197196773 @default.
• W2898565265 cites W2409027410 @default.
• W2898565265 cites W2509363289 @default.
• W2898565265 cites W2535160676 @default.
• W2898565265 cites W2876707141 @default.
• W2898565265 doi "https://doi.org/10.1124/mol.118.113514" @default.
• W2898565265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30355744" @default.
• W2898565265 hasPublicationYear "2018" @default.
• W2898565265 type Work @default.
• W2898565265 sameAs 2898565265 @default.
• W2898565265 citedByCount "4" @default.
• W2898565265 countsByYear W28985652652020 @default.
• W2898565265 countsByYear W28985652652023 @default.
• W2898565265 crossrefType "journal-article" @default.
• W2898565265 hasAuthorship W2898565265A5007766006 @default.
• W2898565265 hasAuthorship W2898565265A5011521949 @default.
• W2898565265 hasAuthorship W2898565265A5011893460 @default.
• W2898565265 hasAuthorship W2898565265A5018462164 @default.
• W2898565265 hasAuthorship W2898565265A5021026258 @default.
• W2898565265 hasAuthorship W2898565265A5064438527 @default.
• W2898565265 hasAuthorship W2898565265A5083880434 @default.
• W2898565265 hasBestOaLocation W28985652651 @default.
• W2898565265 hasConcept C104317684 @default.
• W2898565265 hasConcept C119062480 @default.
• W2898565265 hasConcept C134018914 @default.
• W2898565265 hasConcept C158617107 @default.
• W2898565265 hasConcept C161573976 @default.
• W2898565265 hasConcept C178790620 @default.
• W2898565265 hasConcept C181199279 @default.
• W2898565265 hasConcept C185592680 @default.
• W2898565265 hasConcept C188053792 @default.
• W2898565265 hasConcept C2776109719 @default.
• W2898565265 hasConcept C2778117581 @default.
• W2898565265 hasConcept C2779306644 @default.
• W2898565265 hasConcept C2779535977 @default.
• W2898565265 hasConcept C537181965 @default.
• W2898565265 hasConcept C55493867 @default.
• W2898565265 hasConcept C86803240 @default.
• W2898565265 hasConcept C95444343 @default.
• W2898565265 hasConceptScore W2898565265C104317684 @default.
• W2898565265 hasConceptScore W2898565265C119062480 @default.
• W2898565265 hasConceptScore W2898565265C134018914 @default.
• W2898565265 hasConceptScore W2898565265C158617107 @default.
• W2898565265 hasConceptScore W2898565265C161573976 @default.
• W2898565265 hasConceptScore W2898565265C178790620 @default.
• W2898565265 hasConceptScore W2898565265C181199279 @default.
• W2898565265 hasConceptScore W2898565265C185592680 @default.
• W2898565265 hasConceptScore W2898565265C188053792 @default.
• W2898565265 hasConceptScore W2898565265C2776109719 @default.
• W2898565265 hasConceptScore W2898565265C2778117581 @default.
• W2898565265 hasConceptScore W2898565265C2779306644 @default.
• W2898565265 hasConceptScore W2898565265C2779535977 @default.
• W2898565265 hasConceptScore W2898565265C537181965 @default.
• W2898565265 hasConceptScore W2898565265C55493867 @default.
• W2898565265 hasConceptScore W2898565265C86803240 @default.
• W2898565265 hasConceptScore W2898565265C95444343 @default.
• W2898565265 hasIssue "1" @default.

• next