Matches in SemOpenAlex for { <https://semopenalex.org/work/W2898844999> ?p ?o ?g. }
- W2898844999 abstract "Abstract Spermatogenesis is the process by which male gametes are formed from a self-renewing population of spermatogonial stem cells (SSCs) residing in the testis. SSCs represent less than 1% of the total testicular cell population, but must achieve a stable balance between self-renewal and differentiation. Once differentiation has occurred, the newly formed and highly proliferative spermatogonia must then enter the meiotic program in which DNA content is doubled, then halved twice to create haploid gametes. While much is known about the critical cellular processes that take place during the specialized cell division that is meiosis, much less is known about how the spermatocytes in the “first-wave” compare to those that contribute to long-term, “steady-state” spermatogenesis. Given the strictly-defined developmental process of spermatogenesis, this study was aimed at exploring the transcriptional profiles of developmental cell stages over the age of the animal. Using a combination of comprehensive germ cell sampling with high-resolution, single-cell-mRNA-sequencing, we have generated a reference dataset of germ cell gene expression. We show that discrete developmental stages possess significant differences in the transcriptional profiles from neonates compared to juveniles and adults. Importantly, these gene expression dynamics are also reflected at the protein level in their respective cell types. We also show differential utilization of many biological pathways with age in both spermatogonia and spermatocytes, demonstrating significantly different underlying gene regulatory programs in these cell types over the course of testis development and spermatogenic waves. This dataset represents the first unbiased sampling of spermatogonia and spermatocytes in the developing testis over developmental age, at high-resolution, single-cell depth. Not only does this analysis reveal previously unknown transcriptional dynamics of a highly transitional cell population, it has also begun to reveal critical differences in biological pathway utilization in developing spermatogonia and spermatocytes, including response to DNA damage and double-strand breaks. Author Summary Spermatogenesis is the process by which male gametes – mature spermatozoa – are produced in the testis. This process requires exquisite control over many developmental transitions, including the self-renewal of the germline stem cell population, commitment to meiosis, and ultimately, spermiogenesis. While much is known about molecular mechanisms regulating single transitions at single time points in the mouse, much less is understood about how the spermatogenic progenitor cells, spermatogonia, or the meiotic cells, spermatocytes, of the testis change over developmental age. Our single-cell-mRNA-sequencing analysis is the first to profile both spermatogonia and spermatocytes from neonatal mice through adulthood, revealing novel gene expression dynamics and differential utilization of biological pathways. These discoveries help us to understand how the spermatogenic progenitors of this population modulate their activity to adapt to a changing testicular environment. Furthermore, they also begin to explain previously-observed differences - and deficiencies - in spermatocytes that are derived from the first “wave” of spermatogenesis. Overall, this dataset is the first of its kind to comprehensively profile gene expression dynamics in male germ cell populations over time, enriching our understanding of the complex and highly-orchestrated process of spermatogenesis." @default.
- W2898844999 created "2018-11-09" @default.
- W2898844999 creator A5027601058 @default.
- W2898844999 creator A5030472889 @default.
- W2898844999 creator A5044853072 @default.
- W2898844999 creator A5048065812 @default.
- W2898844999 creator A5061702711 @default.
- W2898844999 creator A5064675052 @default.
- W2898844999 creator A5067839541 @default.
- W2898844999 date "2018-11-06" @default.
- W2898844999 modified "2023-09-26" @default.
- W2898844999 title "Dynamic transcriptome profiles within spermatogonial and spermatocyte populations during postnatal testis maturation revealed by single-cell sequencing" @default.
- W2898844999 cites W1497633776 @default.
- W2898844999 cites W1516316082 @default.
- W2898844999 cites W1786442921 @default.
- W2898844999 cites W1787536492 @default.
- W2898844999 cites W1831021874 @default.
- W2898844999 cites W1966037550 @default.
- W2898844999 cites W1973276840 @default.
- W2898844999 cites W1975334494 @default.
- W2898844999 cites W1977825177 @default.
- W2898844999 cites W1979291624 @default.
- W2898844999 cites W1981823925 @default.
- W2898844999 cites W1984669813 @default.
- W2898844999 cites W1985751159 @default.
- W2898844999 cites W1986178455 @default.
- W2898844999 cites W1987345634 @default.
- W2898844999 cites W1993491850 @default.
- W2898844999 cites W1997096536 @default.
- W2898844999 cites W1998362960 @default.
- W2898844999 cites W2023070341 @default.
- W2898844999 cites W2036654302 @default.
- W2898844999 cites W2037055772 @default.
- W2898844999 cites W2040970294 @default.
- W2898844999 cites W2044994347 @default.
- W2898844999 cites W2051268087 @default.
- W2898844999 cites W2057308778 @default.
- W2898844999 cites W2060153293 @default.
- W2898844999 cites W2064446297 @default.
- W2898844999 cites W2068497129 @default.
- W2898844999 cites W2080554669 @default.
- W2898844999 cites W2085287554 @default.
- W2898844999 cites W2089108779 @default.
- W2898844999 cites W2091028885 @default.
- W2898844999 cites W2096530329 @default.
- W2898844999 cites W2097948046 @default.
- W2898844999 cites W2102540304 @default.
- W2898844999 cites W2106381356 @default.
- W2898844999 cites W2107139873 @default.
- W2898844999 cites W2107665951 @default.
- W2898844999 cites W2109665403 @default.
- W2898844999 cites W2110804359 @default.
- W2898844999 cites W2111705682 @default.
- W2898844999 cites W2126844356 @default.
- W2898844999 cites W2127911142 @default.
- W2898844999 cites W2130410032 @default.
- W2898844999 cites W2134710117 @default.
- W2898844999 cites W2143301018 @default.
- W2898844999 cites W2143319314 @default.
- W2898844999 cites W2150823208 @default.
- W2898844999 cites W2152461652 @default.
- W2898844999 cites W2159675211 @default.
- W2898844999 cites W2163846802 @default.
- W2898844999 cites W2190545194 @default.
- W2898844999 cites W2312488853 @default.
- W2898844999 cites W2343201819 @default.
- W2898844999 cites W2402275391 @default.
- W2898844999 cites W2603130142 @default.
- W2898844999 cites W2604752301 @default.
- W2898844999 cites W2757457042 @default.
- W2898844999 cites W2766494767 @default.
- W2898844999 cites W2767205153 @default.
- W2898844999 cites W2769348083 @default.
- W2898844999 cites W2785428782 @default.
- W2898844999 cites W2788838846 @default.
- W2898844999 cites W2794480084 @default.
- W2898844999 cites W2849954513 @default.
- W2898844999 cites W2886745040 @default.
- W2898844999 cites W2951506174 @default.
- W2898844999 cites W4234934254 @default.
- W2898844999 doi "https://doi.org/10.1101/464149" @default.
- W2898844999 hasPublicationYear "2018" @default.
- W2898844999 type Work @default.
- W2898844999 sameAs 2898844999 @default.
- W2898844999 citedByCount "1" @default.
- W2898844999 countsByYear W28988449992021 @default.
- W2898844999 crossrefType "posted-content" @default.
- W2898844999 hasAuthorship W2898844999A5027601058 @default.
- W2898844999 hasAuthorship W2898844999A5030472889 @default.
- W2898844999 hasAuthorship W2898844999A5044853072 @default.
- W2898844999 hasAuthorship W2898844999A5048065812 @default.
- W2898844999 hasAuthorship W2898844999A5061702711 @default.
- W2898844999 hasAuthorship W2898844999A5064675052 @default.
- W2898844999 hasAuthorship W2898844999A5067839541 @default.
- W2898844999 hasBestOaLocation W28988449991 @default.
- W2898844999 hasConcept C104317684 @default.
- W2898844999 hasConcept C123765429 @default.
- W2898844999 hasConcept C134018914 @default.
- W2898844999 hasConcept C144024400 @default.
- W2898844999 hasConcept C148196450 @default.