Matches in SemOpenAlex for { <https://semopenalex.org/work/W2898850489> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2898850489 abstract "ABSTRACT Tumours are composed of an array of unique cancer cell clones along with many non-tumour cells such as immune cells, fibroblasts and endothelial cells, which make up the complex tumour microenvironment. To better understand the co-evolution of tumour clones and cells of the tumour microenvironment, we require tools to spatially resolve heterotypic cellular interactions at the single cell level. We present a novel protein-based barcoding technology termed nuclear tandem epitope protein (nTEP) barcoding, which can be designed to combinatorially encode and track dozens to hundreds of tumour clones in their spatial context within complex cellular mixtures using multiplexed antibody-based imaging. Here we provide proof-of-principle of nTEP barcoding and develop the technology, which relies on lentiviral - based stable expression of a nuclear-localised fluorophore that contains unique combinations of protein epitope tags that can be decoded by a limited set of antibodies. By generating a series of cell lines expressing unique nTEP barcodes, we were able to robustly identify and spatially deconvolve specific clones present within highly complex cell mixtures at the single cell level using state-of-the-art iterative indirect immunofluorescence imaging (4i). We define the utility of nTEP-barcoding as a powerful tool for visualising and resolving tumour heterogeneity at the cellular level, and envision its usage in mouse tumour models for understanding how tumour clones modulate and interact with stromal- and immune cells in cancer." @default.
- W2898850489 created "2018-11-09" @default.
- W2898850489 creator A5016730636 @default.
- W2898850489 creator A5041877345 @default.
- W2898850489 creator A5065325263 @default.
- W2898850489 creator A5071250969 @default.
- W2898850489 date "2018-10-30" @default.
- W2898850489 modified "2023-09-24" @default.
- W2898850489 title "Resolving Tumour Clonal Heterogeneity and Spatial Complexity using Nuclear Tandem Epitope Protein (nTEP) Barcoding" @default.
- W2898850489 cites W1979486580 @default.
- W2898850489 cites W1982729887 @default.
- W2898850489 cites W2033278321 @default.
- W2898850489 cites W2042789810 @default.
- W2898850489 cites W2053129129 @default.
- W2898850489 cites W2060469376 @default.
- W2898850489 cites W2107554012 @default.
- W2898850489 cites W2131372145 @default.
- W2898850489 cites W2520858613 @default.
- W2898850489 cites W2550247089 @default.
- W2898850489 cites W2586629973 @default.
- W2898850489 cites W2610509384 @default.
- W2898850489 cites W2793258150 @default.
- W2898850489 cites W2887091285 @default.
- W2898850489 cites W2898075889 @default.
- W2898850489 doi "https://doi.org/10.1101/456855" @default.
- W2898850489 hasPublicationYear "2018" @default.
- W2898850489 type Work @default.
- W2898850489 sameAs 2898850489 @default.
- W2898850489 citedByCount "0" @default.
- W2898850489 crossrefType "posted-content" @default.
- W2898850489 hasAuthorship W2898850489A5016730636 @default.
- W2898850489 hasAuthorship W2898850489A5041877345 @default.
- W2898850489 hasAuthorship W2898850489A5065325263 @default.
- W2898850489 hasAuthorship W2898850489A5071250969 @default.
- W2898850489 hasBestOaLocation W28988504891 @default.
- W2898850489 hasConcept C121608353 @default.
- W2898850489 hasConcept C151730666 @default.
- W2898850489 hasConcept C159654299 @default.
- W2898850489 hasConcept C16930146 @default.
- W2898850489 hasConcept C195616568 @default.
- W2898850489 hasConcept C2779343474 @default.
- W2898850489 hasConcept C502942594 @default.
- W2898850489 hasConcept C54355233 @default.
- W2898850489 hasConcept C70721500 @default.
- W2898850489 hasConcept C86803240 @default.
- W2898850489 hasConcept C95444343 @default.
- W2898850489 hasConcept C96232424 @default.
- W2898850489 hasConceptScore W2898850489C121608353 @default.
- W2898850489 hasConceptScore W2898850489C151730666 @default.
- W2898850489 hasConceptScore W2898850489C159654299 @default.
- W2898850489 hasConceptScore W2898850489C16930146 @default.
- W2898850489 hasConceptScore W2898850489C195616568 @default.
- W2898850489 hasConceptScore W2898850489C2779343474 @default.
- W2898850489 hasConceptScore W2898850489C502942594 @default.
- W2898850489 hasConceptScore W2898850489C54355233 @default.
- W2898850489 hasConceptScore W2898850489C70721500 @default.
- W2898850489 hasConceptScore W2898850489C86803240 @default.
- W2898850489 hasConceptScore W2898850489C95444343 @default.
- W2898850489 hasConceptScore W2898850489C96232424 @default.
- W2898850489 hasLocation W28988504891 @default.
- W2898850489 hasOpenAccess W2898850489 @default.
- W2898850489 hasPrimaryLocation W28988504891 @default.
- W2898850489 hasRelatedWork W1823961035 @default.
- W2898850489 hasRelatedWork W2066059272 @default.
- W2898850489 hasRelatedWork W2123776778 @default.
- W2898850489 hasRelatedWork W2183072594 @default.
- W2898850489 hasRelatedWork W2357525786 @default.
- W2898850489 hasRelatedWork W2399636123 @default.
- W2898850489 hasRelatedWork W2444319756 @default.
- W2898850489 hasRelatedWork W2928062689 @default.
- W2898850489 hasRelatedWork W3196212526 @default.
- W2898850489 hasRelatedWork W4246009453 @default.
- W2898850489 isParatext "false" @default.
- W2898850489 isRetracted "false" @default.
- W2898850489 magId "2898850489" @default.
- W2898850489 workType "article" @default.