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• W2898894059 abstract "Chlorpyrifos (CPF) is one of the most widely used organophosphate insecticides worldwide. In mammals, CPF exposure can affect the peripheral and central nervous systems, which can eventually even lead to death. In the present study, crude enzyme extracts obtained from Alcanivorax borkumensis were used for the degradation of CPF. Various experimental parameters that may affect the enzyme catalysis, such as pH, temperature, substrate (CPF), and enzyme concentrations, were studied along with the kinetics of enzymatic degradation. The major contributor toward the degradation of CPF could possibly be alkane hydroxylases and lipases, which might have contributed to the simultaneous degradation of alkane hydroxylase transformation products. A maximum of 95% degradation in 6 h was observed at 50 ppm of CPF. Michaelis–Menten kinetics was applied to study the enzymatic degradation. From the Lineweaver-Burk plot, the V max was calculated to be 1.06 mg L-1 h-1 and the K m was calculated to be 20.52 mg. A pH of 2 and temperature of 40 °C were found to be optimal for the effective CPF degradation. Moreover, increased enzyme activities (concentrations) had minimal effect on CPF degradation. An ~800% increase in enzyme activity resulted in only an ~20% increase in degradation of CPF. The current study provides insight into organophosphate pesticides biodegradation; further studies are required for the environmental applications of these enzymes in CPF-contaminated soils and water resources." @default.
• W2898894059 created "2018-11-09" @default.
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• W2898894059 date "2018-01-01" @default.
• W2898894059 modified "2023-10-16" @default.
• W2898894059 title "Chlorpyrifos Degradation by Crude Enzyme Extracts Obtained from<i>Alcanivorax borkumensis</i>" @default.
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• W2898894059 doi "https://doi.org/10.1021/bk-2018-1302.ch004" @default.
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