FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2898895968> ?p ?o ?g. }

• W2898895968 endingPage "110" @default.
• W2898895968 startingPage "98" @default.
• W2898895968 abstract "Assessment of viral contamination is essential for monitoring the microbial quality of water and protection of public health, as human virus presence is not accurately determined using bacterial indicators. Currently, the time required for conventional viral testing means that water contaminated with human pathogens may be used (e.g. for drinking, recreation or irrigation) days before results are available. Here we report a new rapid method for coliphage enumeration, the QuantiPhage (QP) assay. The novelty of the assay is the use of cellulose absorbent pad materials to support coliphage growth and colorimetric detection, in place of agar that is used in the plaque assay. In addition to saving time associated with agar preparation and tempering, the QP assay enabled enumeration of somatic coliphages in 1.5–2 h and F+ coliphages in 2.5–3 h. The assays were highly sensitive, with a lower detection limit of 1 plaque forming unit (PFU) per mL where 1 mL sample volumes were analysed, and 1 PFU per 10 mL where 10 mL sample volumes were analysed. This is the first rapid culture assay to enable low numbers of coliphages to be reliably detected and to produce directly equivalent results to agar-based plaque assays. A novel gelatin-immobilisation method is also reported, that reduces time to prepare bacterial cells from ∼20 h to 40–60 min (depending on the assay format), and provides a ready to use form of cells, that is compatible with rapid detection and kit formats. When applied to analysis of somatic coliphages in wastewater samples and surface water samples, mean differences in results of the QP assay and the conventional plaque assay were not statistically significant (mean difference ≤ 0.15 log10 PFU/L and 0.5 PFU/10 mL respectively, P > 0.05). The QP is a valuable tool for assessing microbial water quality, which may assist in improving the management of water resources." @default.
• W2898895968 created "2018-11-09" @default.
• W2898895968 creator A5073193965 @default.
• W2898895968 creator A5082622245 @default.
• W2898895968 date "2019-02-01" @default.
• W2898895968 modified "2023-09-24" @default.
• W2898895968 title "The QuantiPhage assay: A novel method for the rapid colorimetric detection of coliphages using cellulose pad materials" @default.
• W2898895968 cites W1500289635 @default.
• W2898895968 cites W1549281306 @default.
• W2898895968 cites W1571835453 @default.
• W2898895968 cites W1597096457 @default.
• W2898895968 cites W1966217406 @default.
• W2898895968 cites W1973346556 @default.
• W2898895968 cites W1974703021 @default.
• W2898895968 cites W1978719141 @default.
• W2898895968 cites W1987369499 @default.
• W2898895968 cites W1993726271 @default.
• W2898895968 cites W1997934090 @default.
• W2898895968 cites W2007555612 @default.
• W2898895968 cites W2015795623 @default.
• W2898895968 cites W2016606634 @default.
• W2898895968 cites W2024930580 @default.
• W2898895968 cites W2035286761 @default.
• W2898895968 cites W2044667041 @default.
• W2898895968 cites W2045485425 @default.
• W2898895968 cites W2054482591 @default.
• W2898895968 cites W2070951530 @default.
• W2898895968 cites W2075255587 @default.
• W2898895968 cites W2083364167 @default.
• W2898895968 cites W2091007971 @default.
• W2898895968 cites W2093711701 @default.
• W2898895968 cites W2101944667 @default.
• W2898895968 cites W2127221398 @default.
• W2898895968 cites W2142047112 @default.
• W2898895968 cites W2143862901 @default.
• W2898895968 cites W2156824969 @default.
• W2898895968 cites W2163519608 @default.
• W2898895968 cites W2172028517 @default.
• W2898895968 cites W2208118611 @default.
• W2898895968 cites W2316763109 @default.
• W2898895968 cites W2392912007 @default.
• W2898895968 cites W2525817998 @default.
• W2898895968 cites W2613243903 @default.
• W2898895968 cites W2749933703 @default.
• W2898895968 cites W2759193908 @default.
• W2898895968 cites W2764048730 @default.
• W2898895968 cites W767157238 @default.
• W2898895968 doi "https://doi.org/10.1016/j.watres.2018.10.089" @default.
• W2898895968 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30423504" @default.
• W2898895968 hasPublicationYear "2019" @default.
• W2898895968 type Work @default.
• W2898895968 sameAs 2898895968 @default.
• W2898895968 citedByCount "10" @default.
• W2898895968 countsByYear W28988959682020 @default.
• W2898895968 countsByYear W28988959682021 @default.
• W2898895968 countsByYear W28988959682022 @default.
• W2898895968 crossrefType "journal-article" @default.
• W2898895968 hasAuthorship W2898895968A5073193965 @default.
• W2898895968 hasAuthorship W2898895968A5082622245 @default.
• W2898895968 hasConcept C104317684 @default.
• W2898895968 hasConcept C112570922 @default.
• W2898895968 hasConcept C114614502 @default.
• W2898895968 hasConcept C119128265 @default.
• W2898895968 hasConcept C156340839 @default.
• W2898895968 hasConcept C159047783 @default.
• W2898895968 hasConcept C185592680 @default.
• W2898895968 hasConcept C18903297 @default.
• W2898895968 hasConcept C192285135 @default.
• W2898895968 hasConcept C2522874641 @default.
• W2898895968 hasConcept C2776336130 @default.
• W2898895968 hasConcept C2776441376 @default.
• W2898895968 hasConcept C2778660310 @default.
• W2898895968 hasConcept C31903555 @default.
• W2898895968 hasConcept C33923547 @default.
• W2898895968 hasConcept C43617362 @default.
• W2898895968 hasConcept C523546767 @default.
• W2898895968 hasConcept C54355233 @default.
• W2898895968 hasConcept C547475151 @default.
• W2898895968 hasConcept C55493867 @default.
• W2898895968 hasConcept C56684591 @default.
• W2898895968 hasConcept C62643968 @default.
• W2898895968 hasConcept C70913881 @default.
• W2898895968 hasConcept C86803240 @default.
• W2898895968 hasConcept C89423630 @default.
• W2898895968 hasConceptScore W2898895968C104317684 @default.
• W2898895968 hasConceptScore W2898895968C112570922 @default.
• W2898895968 hasConceptScore W2898895968C114614502 @default.
• W2898895968 hasConceptScore W2898895968C119128265 @default.
• W2898895968 hasConceptScore W2898895968C156340839 @default.
• W2898895968 hasConceptScore W2898895968C159047783 @default.
• W2898895968 hasConceptScore W2898895968C185592680 @default.
• W2898895968 hasConceptScore W2898895968C18903297 @default.
• W2898895968 hasConceptScore W2898895968C192285135 @default.
• W2898895968 hasConceptScore W2898895968C2522874641 @default.
• W2898895968 hasConceptScore W2898895968C2776336130 @default.
• W2898895968 hasConceptScore W2898895968C2776441376 @default.
• W2898895968 hasConceptScore W2898895968C2778660310 @default.
• W2898895968 hasConceptScore W2898895968C31903555 @default.

• next