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- W2899096728 abstract "Significance An integrative approach is presented for studying cell biology in vivo, assessing protein dynamics and cell behavior, and offering in situ analyses of cytokinesis, daughter-cell polarity, and stereotyped tissue morphogenesis. Tagging endogenous Discs Large 1 (Dlg1) in cartilage using intrabody technology permits in situ 3D time-lapse imaging and reveals Dlg1 enrichment at the midbody during cytokinesis. Functional significance is tested by disrupting Dlg1 multimerization and its midbody localization by using an ablating intrabody, DLGE3. Building on prior work on Dlg1 in epithelia, our work reveals that Dlg1 propagates cell polarity in proliferative mesenchymal tissues and suggests that multiple mechanisms act in concert at distinct phases of the cell cycle to transmit and maintain cell polarity." @default.
- W2899096728 created "2018-11-09" @default.
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- W2899096728 date "2018-10-30" @default.
- W2899096728 modified "2023-09-23" @default.
- W2899096728 title "Discs large 1 controls daughter-cell polarity after cytokinesis in vertebrate morphogenesis" @default.
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- W2899096728 doi "https://doi.org/10.1073/pnas.1713959115" @default.
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