Matches in SemOpenAlex for { <https://semopenalex.org/work/W2899151145> ?p ?o ?g. }
- W2899151145 endingPage "752.e9" @default.
- W2899151145 startingPage "739" @default.
- W2899151145 abstract "The RING E3 ubiquitin ligase UHRF1 controls DNA methylation through its ability to target the maintenance DNA methyltransferase DNMT1 to newly replicated chromatin. DNMT1 recruitment relies on ubiquitylation of histone H3 by UHRF1; however, how UHRF1 deposits ubiquitin onto the histone is unknown. Here, we demonstrate that the ubiquitin-like domain (UBL) of UHRF1 is essential for RING-mediated H3 ubiquitylation. Using chemical crosslinking and mass spectrometry, biochemical assays, and recombinant chromatin substrates, we show that the UBL participates in structural rearrangements of UHRF1 upon binding to chromatin and the E2 ubiquitin conjugating enzyme UbcH5a/UBE2D1. Similar to ubiquitin, the UBL exerts its effects through a hydrophobic patch that contacts a regulatory surface on the “backside” of the E2 to stabilize the E2-E3-chromatin complex. Our analysis of the enzymatic mechanism of UHRF1 uncovers an unexpected function of the UBL domain and defines a new role for this domain in DNMT1-dependent inheritance of DNA methylation." @default.
- W2899151145 created "2018-11-09" @default.
- W2899151145 creator A5005478591 @default.
- W2899151145 creator A5021135041 @default.
- W2899151145 creator A5026411335 @default.
- W2899151145 creator A5026468776 @default.
- W2899151145 creator A5033138055 @default.
- W2899151145 creator A5035658278 @default.
- W2899151145 creator A5064607453 @default.
- W2899151145 date "2018-11-01" @default.
- W2899151145 modified "2023-10-16" @default.
- W2899151145 title "Critical Role of the UBL Domain in Stimulating the E3 Ubiquitin Ligase Activity of UHRF1 toward Chromatin" @default.
- W2899151145 cites W1644001471 @default.
- W2899151145 cites W1751741427 @default.
- W2899151145 cites W1805095076 @default.
- W2899151145 cites W1977063334 @default.
- W2899151145 cites W1977709885 @default.
- W2899151145 cites W2003314433 @default.
- W2899151145 cites W2006410633 @default.
- W2899151145 cites W2021803200 @default.
- W2899151145 cites W2027583609 @default.
- W2899151145 cites W2028111548 @default.
- W2899151145 cites W2029794768 @default.
- W2899151145 cites W2030775866 @default.
- W2899151145 cites W2037586946 @default.
- W2899151145 cites W2045174330 @default.
- W2899151145 cites W2059890127 @default.
- W2899151145 cites W2075459950 @default.
- W2899151145 cites W2077561581 @default.
- W2899151145 cites W2087087669 @default.
- W2899151145 cites W2095348121 @default.
- W2899151145 cites W2106889224 @default.
- W2899151145 cites W2112013199 @default.
- W2899151145 cites W2121264387 @default.
- W2899151145 cites W2131250305 @default.
- W2899151145 cites W2131612302 @default.
- W2899151145 cites W2142745453 @default.
- W2899151145 cites W2150194502 @default.
- W2899151145 cites W2163078458 @default.
- W2899151145 cites W2209705351 @default.
- W2899151145 cites W2273372803 @default.
- W2899151145 cites W2313088239 @default.
- W2899151145 cites W2508535406 @default.
- W2899151145 cites W2517540767 @default.
- W2899151145 cites W2554647387 @default.
- W2899151145 cites W2594502891 @default.
- W2899151145 cites W2743528552 @default.
- W2899151145 cites W2766763178 @default.
- W2899151145 cites W2784474489 @default.
- W2899151145 cites W2790058421 @default.
- W2899151145 cites W2899101716 @default.
- W2899151145 cites W4246176234 @default.
- W2899151145 doi "https://doi.org/10.1016/j.molcel.2018.09.028" @default.
- W2899151145 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6242706" @default.
- W2899151145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30392929" @default.
- W2899151145 hasPublicationYear "2018" @default.
- W2899151145 type Work @default.
- W2899151145 sameAs 2899151145 @default.
- W2899151145 citedByCount "57" @default.
- W2899151145 countsByYear W28991511452018 @default.
- W2899151145 countsByYear W28991511452019 @default.
- W2899151145 countsByYear W28991511452020 @default.
- W2899151145 countsByYear W28991511452021 @default.
- W2899151145 countsByYear W28991511452022 @default.
- W2899151145 countsByYear W28991511452023 @default.
- W2899151145 crossrefType "journal-article" @default.
- W2899151145 hasAuthorship W2899151145A5005478591 @default.
- W2899151145 hasAuthorship W2899151145A5021135041 @default.
- W2899151145 hasAuthorship W2899151145A5026411335 @default.
- W2899151145 hasAuthorship W2899151145A5026468776 @default.
- W2899151145 hasAuthorship W2899151145A5033138055 @default.
- W2899151145 hasAuthorship W2899151145A5035658278 @default.
- W2899151145 hasAuthorship W2899151145A5064607453 @default.
- W2899151145 hasBestOaLocation W28991511451 @default.
- W2899151145 hasConcept C104317684 @default.
- W2899151145 hasConcept C134459356 @default.
- W2899151145 hasConcept C150194340 @default.
- W2899151145 hasConcept C153911025 @default.
- W2899151145 hasConcept C175211621 @default.
- W2899151145 hasConcept C190727270 @default.
- W2899151145 hasConcept C25602115 @default.
- W2899151145 hasConcept C2779979206 @default.
- W2899151145 hasConcept C33288867 @default.
- W2899151145 hasConcept C552990157 @default.
- W2899151145 hasConcept C55493867 @default.
- W2899151145 hasConcept C64927066 @default.
- W2899151145 hasConcept C83640560 @default.
- W2899151145 hasConcept C86803240 @default.
- W2899151145 hasConcept C91965660 @default.
- W2899151145 hasConcept C95444343 @default.
- W2899151145 hasConceptScore W2899151145C104317684 @default.
- W2899151145 hasConceptScore W2899151145C134459356 @default.
- W2899151145 hasConceptScore W2899151145C150194340 @default.
- W2899151145 hasConceptScore W2899151145C153911025 @default.
- W2899151145 hasConceptScore W2899151145C175211621 @default.
- W2899151145 hasConceptScore W2899151145C190727270 @default.
- W2899151145 hasConceptScore W2899151145C25602115 @default.
- W2899151145 hasConceptScore W2899151145C2779979206 @default.