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• W2899172407 abstract "Abstract On‐chip generation of pressure gradients via electrokinetic means can offer several advantages to microfluidic assay design and operation in a variety of applications. In this article, we describe a simple approach to realizing this capability by employing a polyacrylamide‐based gel structure fabricated within a fluid reservoir located at the terminating end of a microchannel. Application of an electric field across this membrane has been shown to block a majority of the electroosmotic flow generated within the open duct yielding a high pressure at the channel–membrane junction. Experiments show the realization of higher pressure‐driven velocities in an electric field‐free separation channel integrated to the micropump with this design compared to other similar micropumps described in the literature. In addition, the noted velocity was found to be less sensitive to the extent of Debye layer overlap in the channel network, and therefore more impressive when working with background electrolytes having higher ionic strengths. With the current system, pressure‐driven velocities up to 3.6 mm/s were realized in a 300‐nm‐deep separation channel applying a maximum voltage of 3 kV at a channel terminal. To demonstrate the separative performance of our device, a nanofluidic pressure‐driven ion‐chromatographic analysis was subsequently implemented that relied on the slower migration of cationic analytes relative to the neutral and anionic ones in the separation channel likely due to their strong electrostatic interaction with the channel surface charges. A mixture of amino acids was thus separated with resolutions greater than those reported by our group for a similar analysis previously." @default.
• W2899172407 created "2018-11-09" @default.
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• W2899172407 date "2018-11-06" @default.
• W2899172407 modified "2023-10-17" @default.
• W2899172407 title "On‐chip pressure generation using a gel membrane fabricated outside of the microfluidic network" @default.
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• W2899172407 doi "https://doi.org/10.1002/elps.201800306" @default.
• W2899172407 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6775627" @default.
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• W2899172407 hasPublicationYear "2018" @default.
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