Matches in SemOpenAlex for { <https://semopenalex.org/work/W2899183229> ?p ?o ?g. }
- W2899183229 endingPage "135" @default.
- W2899183229 startingPage "128" @default.
- W2899183229 abstract "The properties of two-domain laccase of Streptomyces anulatus (SaSL) and its role in transformation of humic acids (HA) from chernozem, sod-podzolic soil and peat at alkaline pH values were studied. The SaSL was cloned, expressed in E. coli and obtained in an electrophoretically homogeneous state. SaSL is an oligomeric protein with a molecular weight of 235-300 kDa and six or eight subunits in the molecule. The molecular weight of the subunit is 37.7 kDa. Its catalytic properties are similar to those of the previously described two-domain laccase. The enzyme catalyzed oxidation of electron donors (K4[Fe(CN)6], ABTS) at acidic pH and phenolic substrates (2-methoxyphenol, 2,6-dimethixyphenol) at alkaline pH values. The efficiency of catalysis was higher in the case of electron donors than phenolic substrates. The enzyme showed a high thermal stability and was more stable at neutral and alkaline pH values. The enzyme effectively transformed humic acids at alkaline pH values. It was found that polymerization reactions took place during interaction of SaSL with HA, as well as with their high-molecular weight (>80 kDa) and low-molecular weight (<5 kDa) fractions. Our results suggest a possible involvement of the two-domaim laccases in humification processes in alkaline soils." @default.
- W2899183229 created "2018-11-09" @default.
- W2899183229 creator A5008746568 @default.
- W2899183229 creator A5049387724 @default.
- W2899183229 creator A5075405213 @default.
- W2899183229 creator A5085438372 @default.
- W2899183229 creator A5091102342 @default.
- W2899183229 creator A5067624595 @default.
- W2899183229 date "2019-01-01" @default.
- W2899183229 modified "2023-10-01" @default.
- W2899183229 title "Transformation of humic acids by two-domain laccase from Streptomyces anulatus" @default.
- W2899183229 cites W1556306584 @default.
- W2899183229 cites W1880901298 @default.
- W2899183229 cites W1964902763 @default.
- W2899183229 cites W1965034600 @default.
- W2899183229 cites W1973798934 @default.
- W2899183229 cites W1978760477 @default.
- W2899183229 cites W1980504998 @default.
- W2899183229 cites W1995313039 @default.
- W2899183229 cites W1997685169 @default.
- W2899183229 cites W1998854121 @default.
- W2899183229 cites W2005695832 @default.
- W2899183229 cites W2006117571 @default.
- W2899183229 cites W2008532143 @default.
- W2899183229 cites W2008727578 @default.
- W2899183229 cites W2010929717 @default.
- W2899183229 cites W2014614948 @default.
- W2899183229 cites W2015119856 @default.
- W2899183229 cites W2015883187 @default.
- W2899183229 cites W2017886962 @default.
- W2899183229 cites W2019125563 @default.
- W2899183229 cites W2027933379 @default.
- W2899183229 cites W2029077706 @default.
- W2899183229 cites W2032505553 @default.
- W2899183229 cites W2033763049 @default.
- W2899183229 cites W2039121788 @default.
- W2899183229 cites W2041018709 @default.
- W2899183229 cites W2042478355 @default.
- W2899183229 cites W2044172124 @default.
- W2899183229 cites W2046447431 @default.
- W2899183229 cites W2054443612 @default.
- W2899183229 cites W2063426803 @default.
- W2899183229 cites W2066948618 @default.
- W2899183229 cites W2072783679 @default.
- W2899183229 cites W2076896030 @default.
- W2899183229 cites W2078544899 @default.
- W2899183229 cites W2086827209 @default.
- W2899183229 cites W2096401225 @default.
- W2899183229 cites W2098703306 @default.
- W2899183229 cites W2101125241 @default.
- W2899183229 cites W2101606746 @default.
- W2899183229 cites W2118192179 @default.
- W2899183229 cites W2146764279 @default.
- W2899183229 cites W2152427257 @default.
- W2899183229 cites W2157129622 @default.
- W2899183229 cites W2162686899 @default.
- W2899183229 cites W2323241756 @default.
- W2899183229 cites W2569804848 @default.
- W2899183229 cites W2586415152 @default.
- W2899183229 cites W2606286532 @default.
- W2899183229 cites W2767800488 @default.
- W2899183229 cites W2925964618 @default.
- W2899183229 cites W40294199 @default.
- W2899183229 doi "https://doi.org/10.1016/j.procbio.2018.11.001" @default.
- W2899183229 hasPublicationYear "2019" @default.
- W2899183229 type Work @default.
- W2899183229 sameAs 2899183229 @default.
- W2899183229 citedByCount "15" @default.
- W2899183229 countsByYear W28991832292019 @default.
- W2899183229 countsByYear W28991832292020 @default.
- W2899183229 countsByYear W28991832292021 @default.
- W2899183229 countsByYear W28991832292022 @default.
- W2899183229 countsByYear W28991832292023 @default.
- W2899183229 crossrefType "journal-article" @default.
- W2899183229 hasAuthorship W2899183229A5008746568 @default.
- W2899183229 hasAuthorship W2899183229A5049387724 @default.
- W2899183229 hasAuthorship W2899183229A5067624595 @default.
- W2899183229 hasAuthorship W2899183229A5075405213 @default.
- W2899183229 hasAuthorship W2899183229A5085438372 @default.
- W2899183229 hasAuthorship W2899183229A5091102342 @default.
- W2899183229 hasConcept C159750122 @default.
- W2899183229 hasConcept C161790260 @default.
- W2899183229 hasConcept C178790620 @default.
- W2899183229 hasConcept C181199279 @default.
- W2899183229 hasConcept C185592680 @default.
- W2899183229 hasConcept C18903297 @default.
- W2899183229 hasConcept C2778004101 @default.
- W2899183229 hasConcept C2778589381 @default.
- W2899183229 hasConcept C2781031786 @default.
- W2899183229 hasConcept C2781401929 @default.
- W2899183229 hasConcept C52201283 @default.
- W2899183229 hasConcept C76935637 @default.
- W2899183229 hasConcept C86803240 @default.
- W2899183229 hasConceptScore W2899183229C159750122 @default.
- W2899183229 hasConceptScore W2899183229C161790260 @default.
- W2899183229 hasConceptScore W2899183229C178790620 @default.
- W2899183229 hasConceptScore W2899183229C181199279 @default.
- W2899183229 hasConceptScore W2899183229C185592680 @default.