Matches in SemOpenAlex for { <https://semopenalex.org/work/W2899193750> ?p ?o ?g. }
- W2899193750 abstract "Abstract We have employed two complementary high-resolution electron paramagnetic resonance (EPR) techniques with a bifunctional spin label (BSL) to test and refine protein structural models based on crystal structures and cryo-EM. We demonstrate this approach by investigating the effects of nucleotide binding on the structure of myosin’s catalytic domain (CD), while myosin is in complex with actin. Unlike conventional spin labels attached to single Cys, BSL reacts with a pair of Cys; in this study, we thoroughly characterize BSL’s rigid, highly stereoselective attachment to protein α-helices, which permits accurate measurements of orientation and distance. Distance constraints were obtained from double electron-electron resonance (DEER) on myosin constructs labeled with BSL specifically at two sites. Constraints for orientation of individual helices were obtained previously from continuous-wave EPR (CW-EPR) of myosin labeled at specific sites with BSL in oriented muscle fibers. We have shown previously that CW-EPR of BSL quantifies helix orientation within actin-bound myosin; here we show that the addition of high-resolution distance constraints by DEER alleviates remaining spatial ambiguity, allowing for direct testing and refinement of atomic structural models. This approach is applicable to any orientable complex (e.g., membranes or filaments) in which site-specific di- Cys mutation is feasible." @default.
- W2899193750 created "2018-11-09" @default.
- W2899193750 creator A5019616625 @default.
- W2899193750 creator A5022975491 @default.
- W2899193750 creator A5063616345 @default.
- W2899193750 date "2018-10-31" @default.
- W2899193750 modified "2023-09-26" @default.
- W2899193750 title "High-resolution models of actin-bound myosin from EPR of a bifunctional spin label" @default.
- W2899193750 cites W1499343594 @default.
- W2899193750 cites W1533349405 @default.
- W2899193750 cites W1598053850 @default.
- W2899193750 cites W1821679912 @default.
- W2899193750 cites W1862864832 @default.
- W2899193750 cites W1968266458 @default.
- W2899193750 cites W1977609596 @default.
- W2899193750 cites W1981954173 @default.
- W2899193750 cites W1983889563 @default.
- W2899193750 cites W1988093831 @default.
- W2899193750 cites W1990561548 @default.
- W2899193750 cites W1997910573 @default.
- W2899193750 cites W2005949513 @default.
- W2899193750 cites W2010695307 @default.
- W2899193750 cites W2015223366 @default.
- W2899193750 cites W2022378428 @default.
- W2899193750 cites W2025346903 @default.
- W2899193750 cites W2026145805 @default.
- W2899193750 cites W2029667189 @default.
- W2899193750 cites W2038862514 @default.
- W2899193750 cites W2039241916 @default.
- W2899193750 cites W2040899301 @default.
- W2899193750 cites W2041625063 @default.
- W2899193750 cites W2042651221 @default.
- W2899193750 cites W2043664506 @default.
- W2899193750 cites W2044629144 @default.
- W2899193750 cites W2046167970 @default.
- W2899193750 cites W2056126751 @default.
- W2899193750 cites W2056148801 @default.
- W2899193750 cites W2058371000 @default.
- W2899193750 cites W2059820101 @default.
- W2899193750 cites W2069953906 @default.
- W2899193750 cites W2072529314 @default.
- W2899193750 cites W2083369708 @default.
- W2899193750 cites W2085239860 @default.
- W2899193750 cites W2087837838 @default.
- W2899193750 cites W2098513990 @default.
- W2899193750 cites W2099127313 @default.
- W2899193750 cites W2100954617 @default.
- W2899193750 cites W2101463683 @default.
- W2899193750 cites W2102997141 @default.
- W2899193750 cites W2116675254 @default.
- W2899193750 cites W2118200955 @default.
- W2899193750 cites W2120259237 @default.
- W2899193750 cites W2124026197 @default.
- W2899193750 cites W2129434797 @default.
- W2899193750 cites W2131830655 @default.
- W2899193750 cites W2143205915 @default.
- W2899193750 cites W2144321597 @default.
- W2899193750 cites W2149298576 @default.
- W2899193750 cites W2149525061 @default.
- W2899193750 cites W2152301430 @default.
- W2899193750 cites W2152307221 @default.
- W2899193750 cites W2154663686 @default.
- W2899193750 cites W2194535754 @default.
- W2899193750 cites W2243195272 @default.
- W2899193750 cites W2273975555 @default.
- W2899193750 cites W2315787811 @default.
- W2899193750 cites W2416915225 @default.
- W2899193750 cites W2438805704 @default.
- W2899193750 cites W2474486403 @default.
- W2899193750 cites W2949351159 @default.
- W2899193750 cites W336760737 @default.
- W2899193750 doi "https://doi.org/10.1101/458257" @default.
- W2899193750 hasPublicationYear "2018" @default.
- W2899193750 type Work @default.
- W2899193750 sameAs 2899193750 @default.
- W2899193750 citedByCount "2" @default.
- W2899193750 countsByYear W28991937502019 @default.
- W2899193750 crossrefType "posted-content" @default.
- W2899193750 hasAuthorship W2899193750A5019616625 @default.
- W2899193750 hasAuthorship W2899193750A5022975491 @default.
- W2899193750 hasAuthorship W2899193750A5063616345 @default.
- W2899193750 hasBestOaLocation W28991937501 @default.
- W2899193750 hasConcept C121332964 @default.
- W2899193750 hasConcept C12554922 @default.
- W2899193750 hasConcept C125705527 @default.
- W2899193750 hasConcept C126838900 @default.
- W2899193750 hasConcept C143409427 @default.
- W2899193750 hasConcept C161790260 @default.
- W2899193750 hasConcept C16345878 @default.
- W2899193750 hasConcept C177462420 @default.
- W2899193750 hasConcept C185592680 @default.
- W2899193750 hasConcept C187961010 @default.
- W2899193750 hasConcept C18903297 @default.
- W2899193750 hasConcept C193812863 @default.
- W2899193750 hasConcept C2524010 @default.
- W2899193750 hasConcept C2778530040 @default.
- W2899193750 hasConcept C2779965526 @default.
- W2899193750 hasConcept C2780443040 @default.
- W2899193750 hasConcept C33923547 @default.
- W2899193750 hasConcept C46141821 @default.