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• W2899202407 abstract "Abstract Experimental evolution of Escherichia coli in one month long stationary-phase in lysogeny broth batch cultures repeatedly selected mutations in the genes for the stationary-phase sigma factor RpoS and the cAMP phosphodiesterase CpdA. The founder strain carried a previously identified allele of rpoS , referred to as rpoS819, a partially functional variant that confers growth advantage in stationary-phase (GASP). The 46 base duplication at the 3’ end of rpoS819 produces a longer protein present at very low levels compared to wild type RpoS. A new rpoS variant rpoS92 , carrying a re-duplication of the original duplication in rpoS819, arose during the first week of our evolution experiment. In rpoS92 , an in-frame stop codon truncated RpoS819 creating a shorter RpoS92 whose levels are restored to that of wild type RpoS. Transcription profiling of rpoS92 indicated a shift in gene-expression to that of wild-type rpoS , reversing some of the expression trends of rpoS819 . Δ3 cpdA , carrying an in-frame three base deletion, had arisen late in our evolution experiment. It is a loss of function mutation, which elevates cAMP levels. Using mixed culture competition experiments, we demonstrate that rpoS92 confers GASP, whereas Δ3 cpdA confers relatively modest GASP in comparison to the ancestral rpoS819 . Δ3 cpdA mediates epistatic repression of rpoS92 GASP. The original survivor carrying both rpoS92 and Δ3 cpdA besides other mutations displays robust GASP, highlighting the role of these additional mutations in reversing the epistatic interaction between Δ3 cpdA and rpoS92. In 10- and 20-day old spent media, there is a reduction in the competitive fitness of rpoS92, which is arrested by Δ3 cpdA. Thus the activity of RpoS fluctuates via genetic mutations in deep stationary phase, and additional mutations in CpdA helps modulate the competitive fitness of RpoS variants." @default.
• W2899202407 created "2018-11-09" @default.
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• W2899202407 date "2018-11-04" @default.
• W2899202407 modified "2023-09-25" @default.
• W2899202407 title "Modulation ofrpoSfitness by loss ofcpdAactivity during stationary-phase inEscherichia coli" @default.
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• W2899202407 doi "https://doi.org/10.1101/460451" @default.
• W2899202407 hasPublicationYear "2018" @default.
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