Matches in SemOpenAlex for { <https://semopenalex.org/work/W2899255061> ?p ?o ?g. }
- W2899255061 endingPage "e0206221" @default.
- W2899255061 startingPage "e0206221" @default.
- W2899255061 abstract "Ascophyllum nodosum extract (ANE) contains bioactive compounds that improve the growth of Arabidopsis in experimentally-induced saline conditions; however, the molecular mechanisms through which ANE elicits tolerance to salinity remain largely unexplored. Micro RNAs (miRNAs) are key regulators of gene expression, playing crucial roles in plant growth, development, and stress tolerance. Next generation sequencing of miRNAs from leaves of control Arabidopsis and from plants subjected to three treatments (ANE, NaCl and ANE+NaCl) was used to identify ANE-responsive miRNA in the absence and presence of saline conditions. Differential gene expression analysis revealed that ANE had a strong effect on miRNAs expression in both conditions. In the presence of salinity, ANE tended to reduce the up-regulation or the down-regulation trend induced caused by NaCl in miRNAs such as ath-miR396a-5p, ath-miR399, ath-miR2111b and ath-miR827. To further uncover the effects of ANE, the expression of several target genes of a number of ANE-responsive miRNAs was analyzed by qPCR. NaCl, but not ANE, down-regulated miR396a-5p, which negatively regulated the expression of AtGRF7 leading to a higher expression of AtDREB2a and AtRD29 in the presence of ANE+NaCl, as compared to ANE alone. ANE+NaCl initially reduced and then enhanced the expression of ath-miR169g-5p, while the expression of the target genes AtNFYA1 and ATNFYA2, known to be involved in the salinity tolerance mechanism, was increased as compared to ANE or to NaCl treatments. ANE and ANE+NaCl modified the expression of ath-miR399, ath-miR827, ath-miR2111b, and their target genes AtUBC24, AtWAK2, AtSYG1 and At3g27150, suggesting a role of ANE in phosphate homeostasis. In vivo and in vitro experiments confirmed the improved growth of Arabidopsis in presence of ANE, in saline conditions and in phosphate-deprived medium, further substantiating the influence of ANE on a variety of essential physiological processes in Arabidopsis including salinity tolerance and phosphate uptake." @default.
- W2899255061 created "2018-11-09" @default.
- W2899255061 creator A5005360269 @default.
- W2899255061 creator A5007576994 @default.
- W2899255061 creator A5041452448 @default.
- W2899255061 creator A5042968935 @default.
- W2899255061 creator A5074511457 @default.
- W2899255061 creator A5090109461 @default.
- W2899255061 date "2018-10-29" @default.
- W2899255061 modified "2023-10-12" @default.
- W2899255061 title "Ascophyllum nodosum extract mitigates salinity stress in Arabidopsis thaliana by modulating the expression of miRNA involved in stress tolerance and nutrient acquisition" @default.
- W2899255061 cites W1521706556 @default.
- W2899255061 cites W1589457932 @default.
- W2899255061 cites W1837691921 @default.
- W2899255061 cites W1932970392 @default.
- W2899255061 cites W1968351298 @default.
- W2899255061 cites W1970213292 @default.
- W2899255061 cites W1974798506 @default.
- W2899255061 cites W1976665941 @default.
- W2899255061 cites W1980085027 @default.
- W2899255061 cites W1983729367 @default.
- W2899255061 cites W1987438700 @default.
- W2899255061 cites W1991191712 @default.
- W2899255061 cites W1993747291 @default.
- W2899255061 cites W1994470158 @default.
- W2899255061 cites W1998758865 @default.
- W2899255061 cites W2000995197 @default.
- W2899255061 cites W2003112252 @default.
- W2899255061 cites W2005330436 @default.
- W2899255061 cites W2016127075 @default.
- W2899255061 cites W2018756589 @default.
- W2899255061 cites W2021142610 @default.
- W2899255061 cites W2023621850 @default.
- W2899255061 cites W2024556021 @default.
- W2899255061 cites W2027503834 @default.
- W2899255061 cites W2030489111 @default.
- W2899255061 cites W2032663499 @default.
- W2899255061 cites W2035579523 @default.
- W2899255061 cites W2040812694 @default.
- W2899255061 cites W2045381070 @default.
- W2899255061 cites W2046537265 @default.
- W2899255061 cites W2049096796 @default.
- W2899255061 cites W2049098933 @default.
- W2899255061 cites W2056035594 @default.
- W2899255061 cites W2056432773 @default.
- W2899255061 cites W2058788204 @default.
- W2899255061 cites W2060729816 @default.
- W2899255061 cites W2061210125 @default.
- W2899255061 cites W2062203098 @default.
- W2899255061 cites W2065001822 @default.
- W2899255061 cites W2065644053 @default.
- W2899255061 cites W2068685448 @default.
- W2899255061 cites W2073555865 @default.
- W2899255061 cites W2075479129 @default.
- W2899255061 cites W2078862918 @default.
- W2899255061 cites W2078951595 @default.
- W2899255061 cites W2081747395 @default.
- W2899255061 cites W2083945762 @default.
- W2899255061 cites W2085159764 @default.
- W2899255061 cites W2091919915 @default.
- W2899255061 cites W2096703917 @default.
- W2899255061 cites W2101052843 @default.
- W2899255061 cites W2102633756 @default.
- W2899255061 cites W2107277218 @default.
- W2899255061 cites W2116353197 @default.
- W2899255061 cites W2116731237 @default.
- W2899255061 cites W2117021101 @default.
- W2899255061 cites W2121089588 @default.
- W2899255061 cites W2127253105 @default.
- W2899255061 cites W2127446611 @default.
- W2899255061 cites W2127632327 @default.
- W2899255061 cites W2131513403 @default.
- W2899255061 cites W2134132908 @default.
- W2899255061 cites W2135015580 @default.
- W2899255061 cites W2139328007 @default.
- W2899255061 cites W2140537064 @default.
- W2899255061 cites W2143735293 @default.
- W2899255061 cites W2144177541 @default.
- W2899255061 cites W2144934177 @default.
- W2899255061 cites W2145445055 @default.
- W2899255061 cites W2147300417 @default.
- W2899255061 cites W2147804965 @default.
- W2899255061 cites W2149260652 @default.
- W2899255061 cites W2152239989 @default.
- W2899255061 cites W2152871523 @default.
- W2899255061 cites W2156257668 @default.
- W2899255061 cites W2157039533 @default.
- W2899255061 cites W2158838293 @default.
- W2899255061 cites W2160069833 @default.
- W2899255061 cites W2160479334 @default.
- W2899255061 cites W2168925984 @default.
- W2899255061 cites W2188804197 @default.
- W2899255061 cites W2397301551 @default.
- W2899255061 cites W2402309439 @default.
- W2899255061 cites W2511053189 @default.
- W2899255061 cites W2584856753 @default.
- W2899255061 cites W2761663801 @default.
- W2899255061 cites W1964184380 @default.