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- W2899395510 abstract "Aim: To assess whether DNA methylation of monocytes play a role in the development of acute diabetic Charcot foot (CF). Patients & methods: We studied the whole methylome (WM) of circulating monocytes in 18 patients with Type 2 diabetes (T2D) and acute CF, 18 T2D patients with equivalent neuropathy and 18 T2D patients without neuropathy, using the enhanced reduced representation bisulfite sequencing technique. Results & conclusion: WM analysis demonstrated that CF monocytes are differentially methylated compared with non-CF monocytes, in both CpG-site and gene-mapped analysis approaches. Among the methylated genes, several are involved in the migration process during monocyte differentiation into osteoclasts or are indirectly involved through the regulation of inflammatory pathways. Finally, we demonstrated an association between methylation and gene expression in cis- and trans-association." @default.
- W2899395510 created "2018-11-09" @default.
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- W2899395510 date "2019-02-01" @default.
- W2899395510 modified "2023-09-26" @default.
- W2899395510 title "Whole-methylome analysis of circulating monocytes in acute diabetic Charcot foot reveals differentially methylated genes involved in the formation of osteoclasts" @default.
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- W2899395510 doi "https://doi.org/10.2217/epi-2018-0144" @default.
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