FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2899662244> ?p ?o ?g. }

• W2899662244 endingPage "30" @default.
• W2899662244 startingPage "30" @default.
• W2899662244 abstract "Background and Objective: Alterations in gene expressions are often due to epigenetic modifications that can have a significant influence on cancer development, growth, and progression. Lately, histone deacetylase inhibitors (HDACi) such as suberoylanilide hydroxamic acid (SAHA, or vorinostat, MK0683) have been emerging as a new class of drugs with promising therapeutic benefits in controlling cancer growth and metastasis. The small molecule RG7388 (idasanutlin, R05503781) is a newly developed inhibitor that is specific for an oncogene-derived protein called MDM2, which is also in clinical trials for the treatment of various types of cancers. These two drugs have shown the ability to induce p21 expression through distinct mechanisms in MCF-7 and LNCaP cells, which are reported to have wild-type TP53. Our understanding of the molecular mechanism whereby SAHA and RG7388 can induce cell cycle arrest and trigger cell death is still evolving. In this study, we performed experiments to measure the cell cycle arrest effects of SAHA and RG7388 using MCF-7 and LNCaP cells. Materials and Methods: The cytotoxicity, cell cycle arrest, and apoptosis/necroptosis effects of the SAHA and RG7388 treatments were assessed using the Trypan Blue dye exclusion (TBDE) method, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, fluorescence assay with DEVD-amc substrate, and immunoblotting methods. Results: The RG7388 treatment was able to induce cell death by elevating p21WAF1/CIP1 through inhibition of MDM2 in LNCaP, but not in MCF-7 cells, even though there was evidence of p53 elevation. Hence, we suspect that there is some level of uncoupling of p53-mediated transcriptional induction of p21WAF1/CIP1 in MCF-7 cells. Conclusion: Our results from MCF-7 and LNCaP cells confirmed that SAHA and RG7388 treatments were able to induce cell death via a combination of cell cycle arrest and cytotoxic mechanisms. We speculate that our findings could lead to the development of newer treatments for breast and prostate cancers with drug combinations including HDACi." @default.
• W2899662244 created "2018-11-16" @default.
• W2899662244 creator A5022331911 @default.
• W2899662244 creator A5032833963 @default.
• W2899662244 creator A5066406221 @default.
• W2899662244 creator A5066562872 @default.
• W2899662244 creator A5080347343 @default.
• W2899662244 creator A5090480110 @default.
• W2899662244 date "2019-01-29" @default.
• W2899662244 modified "2023-10-16" @default.
• W2899662244 title "Cell Cycle Arrest and Cytotoxic Effects of SAHA and RG7388 Mediated through p21WAF1/CIP1 and p27KIP1 in Cancer Cells" @default.
• W2899662244 cites W1576330810 @default.
• W2899662244 cites W1596376363 @default.
• W2899662244 cites W1974167857 @default.
• W2899662244 cites W1976261939 @default.
• W2899662244 cites W1981524094 @default.
• W2899662244 cites W1983094204 @default.
• W2899662244 cites W1991167758 @default.
• W2899662244 cites W1992762478 @default.
• W2899662244 cites W1996413533 @default.
• W2899662244 cites W1999330214 @default.
• W2899662244 cites W2000913934 @default.
• W2899662244 cites W2003925068 @default.
• W2899662244 cites W2006978594 @default.
• W2899662244 cites W2008836678 @default.
• W2899662244 cites W2029080338 @default.
• W2899662244 cites W2033417821 @default.
• W2899662244 cites W2041713526 @default.
• W2899662244 cites W2041714098 @default.
• W2899662244 cites W2041763256 @default.
• W2899662244 cites W2043960252 @default.
• W2899662244 cites W2046342316 @default.
• W2899662244 cites W2050006906 @default.
• W2899662244 cites W2052516738 @default.
• W2899662244 cites W2052715855 @default.
• W2899662244 cites W2052930096 @default.
• W2899662244 cites W2074336595 @default.
• W2899662244 cites W2082297472 @default.
• W2899662244 cites W2083561654 @default.
• W2899662244 cites W2091151265 @default.
• W2899662244 cites W2094859123 @default.
• W2899662244 cites W2097933346 @default.
• W2899662244 cites W2102458112 @default.
• W2899662244 cites W2106048601 @default.
• W2899662244 cites W2107733280 @default.
• W2899662244 cites W2112351720 @default.
• W2899662244 cites W2116711648 @default.
• W2899662244 cites W2131677957 @default.
• W2899662244 cites W2147215689 @default.
• W2899662244 cites W2151016906 @default.
• W2899662244 cites W2160173183 @default.
• W2899662244 cites W2167543957 @default.
• W2899662244 cites W2327477759 @default.
• W2899662244 cites W2341768654 @default.
• W2899662244 cites W2402709566 @default.
• W2899662244 cites W2418257630 @default.
• W2899662244 cites W2426034980 @default.
• W2899662244 cites W2460318004 @default.
• W2899662244 cites W2604800509 @default.
• W2899662244 cites W2604875090 @default.
• W2899662244 cites W2724336500 @default.
• W2899662244 cites W2774494619 @default.
• W2899662244 cites W2795208162 @default.
• W2899662244 cites W2903655016 @default.
• W2899662244 cites W4211075966 @default.
• W2899662244 cites W4251229106 @default.
• W2899662244 cites W81266651 @default.
• W2899662244 cites W92697522 @default.
• W2899662244 doi "https://doi.org/10.3390/medicina55020030" @default.
• W2899662244 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6409969" @default.
• W2899662244 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30700046" @default.
• W2899662244 hasPublicationYear "2019" @default.
• W2899662244 type Work @default.
• W2899662244 sameAs 2899662244 @default.
• W2899662244 citedByCount "17" @default.
• W2899662244 countsByYear W28996622442020 @default.
• W2899662244 countsByYear W28996622442021 @default.
• W2899662244 countsByYear W28996622442022 @default.
• W2899662244 countsByYear W28996622442023 @default.
• W2899662244 crossrefType "journal-article" @default.
• W2899662244 hasAuthorship W2899662244A5022331911 @default.
• W2899662244 hasAuthorship W2899662244A5032833963 @default.
• W2899662244 hasAuthorship W2899662244A5066406221 @default.
• W2899662244 hasAuthorship W2899662244A5066562872 @default.
• W2899662244 hasAuthorship W2899662244A5080347343 @default.
• W2899662244 hasAuthorship W2899662244A5090480110 @default.
• W2899662244 hasBestOaLocation W28996622441 @default.
• W2899662244 hasConcept C105696609 @default.
• W2899662244 hasConcept C121608353 @default.
• W2899662244 hasConcept C126322002 @default.
• W2899662244 hasConcept C185592680 @default.
• W2899662244 hasConcept C190283241 @default.
• W2899662244 hasConcept C2779723316 @default.
• W2899662244 hasConcept C29537977 @default.
• W2899662244 hasConcept C502942594 @default.
• W2899662244 hasConcept C55493867 @default.
• W2899662244 hasConcept C62112901 @default.
• W2899662244 hasConcept C71924100 @default.

• next