Matches in SemOpenAlex for { <https://semopenalex.org/work/W2899685399> ?p ?o ?g. }
- W2899685399 endingPage "49" @default.
- W2899685399 startingPage "40" @default.
- W2899685399 abstract "The present study is pursuing our previous research, focused on some aspects of Nostoc entophytum ISC32 cell response to the stress caused by exposure to cadmium at the cellular and molecular levels. Variations in the antioxidant system (catalase and ascorbate peroxidase activity) of N. entophytum ISC32 exposed to varying concentrations of Cd (2, and 5 mg/L) resulted in a significant increase in the activity of both catalase and peroxidase. Activity of these enzymes was, however, not significantly changed in the presence of Cd concentrations of 10 and 20 mg/L. Levels of lipid peroxidation, as measured by malondialdehyde (MDA) assay, were observed in response to exposure to Cd (20 mg/L). There was, however, a sharp drop in both antioxidant and lipid peroxidation activities of Cd treated cells after 5 days exposure, likely in consequence of cellular damage. The content of chlorophyll a and phycobiliproteins of living cells were altered under Cd-induced conditions. TEM images of cyanobacterial cells treated with Cd showed cell surface alteration and modification along with altered cellular microcompartments. Cyanobacterial cells treated with Cd at concentrations below the minimum inhibitory concentration (MIC) remained with no apparent structural changes. However, at a higher concentration of Cd (30 mg/L), a clear detachment effect was observed between the mucilage external layer and cell membrane which may be attributed to cell plasmolysis due to toxic effects of Cd. Subsequently, the thickness of the ring-shaped mucilage external layer increased likely as a result of the cell defense mechanisms against toxic concentrations of Cd. Characterization of cells treated with Cd (30 and 150 mg/L) by scanning electron microscopy (SEM) indicated cell shrinkage with varying degrees of distortion and surface wrinkling. Energy-dispersive X-ray spectrometry (EDS) analysis suggested that Cd was not present as nanoparticles within the cell, but in the form of salt or other molecular structures. The up-regulation of chaperons was confirmed for GroEL and HtpG using real-time PCR and northern blot analyses. Interestingly, the expression of GroEL was markedly increased at lower Cd concentration (5 mg/L). However, the ISC32 strain accrued higher levels of HtpG transcript in response to an elevated concentration of Cd (15 mg/L). This pattern seems to be related to the fast and early induction of GroEL, which may be necessary for induction of other factors and heat shock proteins such as HtpG in Cd-treated Nostoc cells. The result of this study paves the way for a more detailed exploration of Cd effects on the defense mechanisms of cyanobacteria. Our research also shed some light on how cyanobacterial cells have evolved to respond to the heavy metal toxicity at the cellular, molecular and ultrastructural levels." @default.
- W2899685399 created "2018-11-16" @default.
- W2899685399 creator A5001823395 @default.
- W2899685399 creator A5007285706 @default.
- W2899685399 creator A5019709779 @default.
- W2899685399 creator A5032780379 @default.
- W2899685399 creator A5060539480 @default.
- W2899685399 creator A5078713078 @default.
- W2899685399 date "2019-03-01" @default.
- W2899685399 modified "2023-10-02" @default.
- W2899685399 title "Nostoc entophytum cell response to cadmium exposure: A possible role of chaperon proteins GroEl and HtpG in cadmium-induced stress" @default.
- W2899685399 cites W1520222297 @default.
- W2899685399 cites W1573518848 @default.
- W2899685399 cites W1845916887 @default.
- W2899685399 cites W1948614239 @default.
- W2899685399 cites W1977125264 @default.
- W2899685399 cites W1983518952 @default.
- W2899685399 cites W1987002374 @default.
- W2899685399 cites W1994004395 @default.
- W2899685399 cites W2019067052 @default.
- W2899685399 cites W2027118002 @default.
- W2899685399 cites W2033700476 @default.
- W2899685399 cites W2044612737 @default.
- W2899685399 cites W2048463548 @default.
- W2899685399 cites W2055083523 @default.
- W2899685399 cites W2059944807 @default.
- W2899685399 cites W2071793550 @default.
- W2899685399 cites W2076801381 @default.
- W2899685399 cites W2089900406 @default.
- W2899685399 cites W2091849413 @default.
- W2899685399 cites W2097890530 @default.
- W2899685399 cites W2101879696 @default.
- W2899685399 cites W2139770049 @default.
- W2899685399 cites W2143444184 @default.
- W2899685399 cites W2157026144 @default.
- W2899685399 cites W2227741506 @default.
- W2899685399 cites W2330633921 @default.
- W2899685399 cites W2595600310 @default.
- W2899685399 cites W2606397059 @default.
- W2899685399 cites W2616600021 @default.
- W2899685399 cites W2617839927 @default.
- W2899685399 cites W2788332779 @default.
- W2899685399 cites W2791149811 @default.
- W2899685399 cites W2793634237 @default.
- W2899685399 cites W2794893825 @default.
- W2899685399 cites W2808818874 @default.
- W2899685399 doi "https://doi.org/10.1016/j.ecoenv.2018.10.104" @default.
- W2899685399 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30419505" @default.
- W2899685399 hasPublicationYear "2019" @default.
- W2899685399 type Work @default.
- W2899685399 sameAs 2899685399 @default.
- W2899685399 citedByCount "12" @default.
- W2899685399 countsByYear W28996853992019 @default.
- W2899685399 countsByYear W28996853992020 @default.
- W2899685399 countsByYear W28996853992021 @default.
- W2899685399 countsByYear W28996853992022 @default.
- W2899685399 countsByYear W28996853992023 @default.
- W2899685399 crossrefType "journal-article" @default.
- W2899685399 hasAuthorship W2899685399A5001823395 @default.
- W2899685399 hasAuthorship W2899685399A5007285706 @default.
- W2899685399 hasAuthorship W2899685399A5019709779 @default.
- W2899685399 hasAuthorship W2899685399A5032780379 @default.
- W2899685399 hasAuthorship W2899685399A5060539480 @default.
- W2899685399 hasAuthorship W2899685399A5078713078 @default.
- W2899685399 hasConcept C178790620 @default.
- W2899685399 hasConcept C185592680 @default.
- W2899685399 hasConcept C2775838275 @default.
- W2899685399 hasConcept C2778004101 @default.
- W2899685399 hasConcept C2778401633 @default.
- W2899685399 hasConcept C2778979269 @default.
- W2899685399 hasConcept C2780369685 @default.
- W2899685399 hasConcept C2780829032 @default.
- W2899685399 hasConcept C544657597 @default.
- W2899685399 hasConcept C55493867 @default.
- W2899685399 hasConcept C86803240 @default.
- W2899685399 hasConceptScore W2899685399C178790620 @default.
- W2899685399 hasConceptScore W2899685399C185592680 @default.
- W2899685399 hasConceptScore W2899685399C2775838275 @default.
- W2899685399 hasConceptScore W2899685399C2778004101 @default.
- W2899685399 hasConceptScore W2899685399C2778401633 @default.
- W2899685399 hasConceptScore W2899685399C2778979269 @default.
- W2899685399 hasConceptScore W2899685399C2780369685 @default.
- W2899685399 hasConceptScore W2899685399C2780829032 @default.
- W2899685399 hasConceptScore W2899685399C544657597 @default.
- W2899685399 hasConceptScore W2899685399C55493867 @default.
- W2899685399 hasConceptScore W2899685399C86803240 @default.
- W2899685399 hasFunder F4320323907 @default.
- W2899685399 hasLocation W28996853991 @default.
- W2899685399 hasLocation W28996853992 @default.
- W2899685399 hasOpenAccess W2899685399 @default.
- W2899685399 hasPrimaryLocation W28996853991 @default.
- W2899685399 hasRelatedWork W1533244249 @default.
- W2899685399 hasRelatedWork W155744969 @default.
- W2899685399 hasRelatedWork W2167392679 @default.
- W2899685399 hasRelatedWork W2183540883 @default.
- W2899685399 hasRelatedWork W2371688276 @default.
- W2899685399 hasRelatedWork W2380016105 @default.
- W2899685399 hasRelatedWork W2383461222 @default.