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- W2899691217 abstract "This study investigated the effects and related mechanisms of ultrasound- and alkali-assisted enzymolysis on production of water-soluble yeast β-glucan (WSYG). Results indicated that ultrasound and alkali pretreatments reduced the particle size of yeast β-glucan (YG) from 8.80 μm to 1.77 and 7.19 μm, respectively. Ultrasound-induced cavitation disrupted YG aggregates to a coarse appearance and exposed internal structure. Alkali penetrated into YG particles and broke the YG aggregates into a flake-like morphology by cleaving the linkages within YG chains. Both pretreatments facilitated enzymolysis by enlarging the YG surface area and increased the WSYG yield to 32.3% and 36.2%, respectively. Meanwhile, the purity of WSYG reached 98.8% after zymoprotein removal by DEAE-Sepharose fast flow column. This work not only provides a green method for producing high-purity and high-yield WSYG, but also reveals the mechanisms of ultrasound and alkali pretreatments for improving enzymolysis efficiency by loosening the YG structure and increasing the surface area." @default.
- W2899691217 created "2018-11-16" @default.
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- W2899691217 date "2019-02-01" @default.
- W2899691217 modified "2023-10-17" @default.
- W2899691217 title "Effects and mechanisms of ultrasound- and alkali-assisted enzymolysis on production of water-soluble yeast β-glucan" @default.
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- W2899691217 doi "https://doi.org/10.1016/j.biortech.2018.11.035" @default.
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