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- W2899837439 abstract "Enzymatic production of chemicals typically includes fermentation of engineered bacteria, preparation of enzymes, and bioconversion processes. Here coupled fermentation-bioconversion process for production of chiral atazanavir intermediate α-chlorohydrin was established. In the fermentation step, the ketoreductase was released from recombinant E. coli cells into medium after high-temperature induction, and the enzyme activity reached 5960 U/mL in the fermentation supernatant. In the bioconversion step, the fermentation supernatant was used directly for conversion of 200 g/L α-chloroketone in heterogeneous reaction mixture, and the residual amount of chloroketone reached less than 0.1% after 24 h of reaction. After filtration and drying, powder α-chlorohydrin was obtained with total yield of 95.7%. Finally, the filtrate of the reaction mixture was used for 2nd batch conversion, and powder α-chlorohydrin was obtained with yield of 95.6%. The above coupled process simplified enzyme preparation procedure, allowed the bioproduction of α-chlorohydrin to be carried out smoothly with reduced waste water discharge, and was advantageous for industrial scale-up." @default.
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- W2899837439 date "2019-01-01" @default.
- W2899837439 modified "2023-10-11" @default.
- W2899837439 title "Coupled fermentation-bioconversion process for production of chiral α-chlorohydrin with recombinant ketoreductase" @default.
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- W2899837439 doi "https://doi.org/10.1016/j.procbio.2018.11.003" @default.
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