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- W2899985168 abstract "Abstract Single molecule RNA fluorescence in situ hybridization (smFISH) has become the standard tool for high spatial resolution analysis of gene expression in the context of tissue organization. This article describes protocols to perform smFISH on whole‐mount mouse embryonic organs, where tissue organization can be compared to RNA expression by co‐immunostaining of known protein markers. An enzymatic labeling strategy is also introduced to produce low‐cost smFISH probes. Important considerations and practical guidelines for imaging smFISH samples using fluorescence confocal microscopy are described. Finally, a suite of custom‐written ImageJ macros is included with detailed instructions to enable semi‐automated smFISH image analysis of both 2D and 3D images. © 2018 by John Wiley & Sons, Inc." @default.
- W2899985168 created "2018-11-16" @default.
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- W2899985168 date "2018-11-05" @default.
- W2899985168 modified "2023-09-27" @default.
- W2899985168 title "Single Molecule RNA FISH (smFISH) in Whole‐Mount Mouse Embryonic Organs" @default.
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- W2899985168 doi "https://doi.org/10.1002/cpcb.79" @default.
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