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• W2900174013 abstract "As the interest in DNA nanotechnology increases, so does the need for larger and more complex DNA structures. In this work, we describe two methods of using large, double-stranded (ds) DNA to self-assemble sequence-specific, nonrepetitive microscale structures. A model system restructures T7 DNA (40 kb) through sequence-specific biotinylation followed by intramolecular binding to a 40 nm diameter neutravidin bead to create T7 rosettes. This model system informed the creation of nodal DNA where nodes with single-stranded DNA flaps are attached to a large dsDNA insert so that a complementary oligonucleotide strap bridges the two nodes for restructuring to form a DNA bolo. To do this in high yield, several methodologies were developed, including a protection/deprotection scheme using RNA/RNase H and dialysis chambers, which remove excess straps while retaining large DNA molecules. To assess these restructuring processes, the DNA was adsorbed onto supported lipid bilayers, allowing for a visual assay of their structure using single-molecule fluorescence microscopy. Good agreement between the expected and observed fluorescence intensity measurements of the individual features of restructured DNA for both the DNA rosettes and bolos gives us a high degree of confidence that both processes give sequence-specific restructuring of large, dsDNA molecules to create microscale objects." @default.
• W2900174013 created "2018-11-16" @default.
• W2900174013 creator A5030154209 @default.
• W2900174013 creator A5041081509 @default.
• W2900174013 creator A5089411486 @default.
• W2900174013 date "2018-11-07" @default.
• W2900174013 modified "2023-10-11" @default.
• W2900174013 title "Microscale Objects via Restructuring of Large, Double-Stranded DNA Molecules" @default.
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• W2900174013 doi "https://doi.org/10.1021/acsami.8b18157" @default.
• W2900174013 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6453721" @default.
• W2900174013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30403478" @default.
• W2900174013 hasPublicationYear "2018" @default.
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